暗视野显微镜

〔结果〕在排除了实验室技术因素后，可采用暗视野显微镜检查、梅毒螺旋体IgM抗体检测、荧光梅毒螺旋体抗体吸收试验，对不确定结果进行确认。

Result After eliminating the laboratory technology factor , the methods ( dark-field microscope , the syphilitic spirochaeta ( IgM ) examination , FTA-ABS ) were conducted in order to confirm indeterminate results .

在暗视野显微镜下观察到的血淋巴中的病毒粒子大小约为0.5μm；

Under a dark-field microscope virus particles were seen as numerous fine particles ( about 0 . 5 / μ m ) in the hemolymph .

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

51 clinical samples were detected by dark-field microscopy and a double-primer PCR , the positive rates were 15.7 % and 19.6 % , respectively .

PCR检测结果，与暗视野显微镜检查结果比较，差异有显著性（P<0.01）；

The result of PCR was compared with the results of dark - field microscopy and there was a statistically significant difference ( P < 0.01 ) .

结果复合PCR检测梅毒与暗视野显微镜检查和梅毒血清学检查有较好的一致性。在总计165例患者中，梅毒51例，生殖器疱疹52例，分别占30.9%和31.5%，未发现软下疳病例。

Results There were 51 ( 30.9 % ) cases of syphilis and 52 ( 31.5 % ) cases of genital herpes among 165 patients , respectively . No chancroid case was found .

方法根据病史、临床表现，结合暗视野显微镜检查、梅毒血清学试验，PCR检测HSV-DNA等进行诊断。结果96例女性生殖器溃疡性疾病患者中，梅毒22例，占22.9%；

Methods 96 female patients were examined for their disease history , clinical features , syphilitic serology and HSV-PCR .

用暗视野显微镜对龈下菌斑的初步观察

Preliminary survey on subgingival plaque by darkfield microscopy

暗视野显微镜在眼科实验研究中的应用

Application of dark field microscope in ophthalmological researches

螺旋体不易观察除非在荧光显微镜或暗视野显微镜观察。

The spirals are not readily seen unless immunofluorescent stain or dark field illumination is employed .

正确的诊断有赖于对可疑患儿的皮疹、胎盘或脐带的渗出物等进行涂片，在暗视野显微镜下用美蓝、嗜银等染色后可检查到梅毒螺旋体。

Pathological diagnosis bases mainly on the finding of spirochete in exudates of exanthema , placenta , or umbilical cord .

3诊断新生儿先天梅毒缺乏金标准，应大力开展19S-IgM-FTA-ABS及暗视野显微镜检查；

There is no golden standard in the diagnosis of congenital syphilis . 19S IgM FTA ABS and dark field microscopy test should be taken ;

最近，通过羊膜穿刺术获得孕妇的羊水，以其作暗视野显微镜观察，对先天性梅毒有诊断价值。

Recently , obtain the amniotic fluid of pregnant woman through amnion puncture art , make observation of dim view microscope with its , have diagnostic value to congenital syphilis .