放射免疫技术
- 网络ria;radio immunoassay ,RIA;radioimmunoassay
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方法:应用酶免疫检测法(ELISA)、聚合酶链反应(PCR)及放射免疫技术分别测定100例原发性肝癌患者血清乙型肝炎病毒指标(HBV-M)及AFP。
Methods : Both HBV-M and AFP in the serum were determinated from 100 cases of patients with PHC by ELISA , PCR and radioimmunoassay .
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方法应用放射免疫技术测定21例胃癌患者胃切除标本组织和血清中IVC的含量以及20例健康者(对照组)血清IVC含量。
Methods Gastric sample tissue and serum content of IV-C in 21 patients with gastric cancer were measured by radioimmunoassay .
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方法用放射免疫技术测定78例老年急性脑梗死患者(脑梗死组)和60名健康老人(对照组)的血浆ET、CGRP和ANP水平,并观察其动态变化。
Methods The concentrations of ET , CGRP and ANP in plasma were determined with radioimmunoassay in 78 elderly patients with acute cerebral infarction and 60 normal controls .
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上述各组均于激发24小时后将豚鼠处死,取其肺组织匀浆,应用放射免疫技术检测其IL-2、IL-6的含量。
The animals of all three groups were killed and the lung tissues were taken out and made into 10 % homogenates respectively . The concentrations of IL-2 and IL-6 in the homogenates were measured by radioimmunity technique .
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采用高效液相色谱电化学检测法(HPLC-ECD)和放射免疫技术测定她们的血清皮质醇、血浆儿茶酚胺CA(NE、E、DA)和5-羟色胺(5-HT)及其代谢产物,和血浆神经肽-Y(NPY)水平。
The plasma catecholamine ( DA , NE , E ), 5-HT , neuropeptide-Y ( NPY ) levels and the serum cortisol level were detected by immunoradiometric assay or high performance liquid chromatography ( HPLC-ED ) .
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进一步,通过Northern杂交和放射免疫技术研究Ad对胶原代谢相关可疑靶基因在mRNA水平和蛋白水平的影响,以探讨Ad在皮肤损伤修复中的作用机制。
Further , the impact of Ad with respect to collagen metabolism interrelated dubious target gene at protein level as well as mRNA level were studied using Northern hybridization and radioimmunoassay technique to investigate the action mechanism of Ad in the restoring of skin injure .
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方法采用免疫印转和放射免疫技术检测了30例VMC、14例DCM患儿血浆中抗心肌线粒体ADP/ATP运载蛋白(ANT)抗体和粒细胞-巨噬细胞集落刺激因子(GM-CSF)。
Methods The plasma autoantibody against myocardiac adenine nucleotide translocator ( ANT ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in children ( 30 with VMC , 14 with DCM ) were studied with western blot and radioimmunoassay .
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再灌注60min后取血,利用放射免疫技术检测大鼠血清细胞因子肿瘤坏死因子α、白细胞介素6和内皮素水平。
After 60-minute reperfusion , blood was gained , and the levels of serum cytokine , tumor necrosis factor alpha , interleukin 6 and endothelin of rats were detected with radioimmunity technology .
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采用放射免疫技术(RIA)测定小白鼠血清胰岛素浓度,照射后16小时降低22.5%.血糖浓度照射后18小时下降9%。
The serum concentration of insulin in mice decreased by 22.5 % after radia - tion for 16 hours , the concentration of blood sugar decreased by 9 % after radiating for 18 hours and the cAMP concentration decreased by 31 % after radiation for 16 hours .
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应用放射免疫技术测定血浆睾酮含量;
Plasma testosterone content ( T ) was detected by RIA ;
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利用放射免疫技术快速检测转基因植物
Rapid Detection of Genetically Modified Plants by Radio-immunity
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应用放射免疫技术对培养液上清液中的胰岛素进行检测和分析。
Insulin secretion was detected by radioimmunoassay technique .
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目的用放射免疫技术测定血药浓度的方法研究猕猴皮下注射重组人集成干扰素α注射液后的药动学。
OBJECTIVE To study pharmacokinetics of recombinant human consensus interferon - α in rhesus monkey by means of radioimmunoassay .
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本实验应用光镜、扫描电镜、透射电镜和放射免疫技术观察了体外培养6天甲状腺细胞形态与功能变化过程。
Morphology and Functions of cultured porcine thyroid cells were studied using light microscopy , electron microscopy and radioimmunoassay .
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利用离体组织培养和放射免疫技术,观察不同浓度的VitB6(0,0.1mg/ml,1mg/ml,10mg/ml和20mg/ml)是否对体外培养大鼠睾丸碎块睾酮的分泌有直接作用。
Observing the effect on T produced by miced-testis of VitB_6 at different concentrations ( 0,0.1 mg / ml 、 1 mg / ml 、 10 mg / ml and 20 mg / ml ) in vitro , by the approachs of tissue culture and radioactivity .
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人血清、尿W物质放射免疫分析技术的建立
Establishment of Radioimmunoassay for Compound W in Human Serum and Urine
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双峰驼排卵机制的研究Ⅰ.促黄体素(LH)的放射免疫测定技术
Studies on the mechanism of ovulation in Bactrian Camel
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同时采用放射免疫分析技术检测健康对照组(15例)的血浆ANP水平。
At the same time , the plasma ANP concentrations were measured by radioimmunoassay in 15 health people as control .
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Bt晶体蛋白Cry1Ac放射免疫检测技术研究
Study on radioimmunoassay of BT cry 1 AC protein
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~(125)I-A蛋白固相放射免疫分析技术及其应用
A solid phase RIA with ~ ( 125 ) i-spa and its application
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方法对70例有不同程度肾小管、肾间质损伤的IgAN患者采用放射免疫分析技术(RIA)测定尿表皮生长因子浓度;
Methods Seventy cases of IgAN with different degree damage of tubules and interstitium were detected for their hEGF urinary concentration by using radioimmunoassay ( RIA ) .
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采用放射免疫分析技术测定患者麻醉前、麻醉后60min、术后5min、术后24h和术后48h中心静脉血皮质醇含量。
The serum cortisol was measured at 5 min before anesthesia , 60 min after anesthesia , 5 min , 24 h and 48 h postoperation by radioimmunoassay .
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方法病因模拟法制作幼龄厌食大鼠模型,用宝宝乐治疗,放射免疫检测技术测定下丘脑、胃窦部及外周血胃泌素、-βEP和CCK的含量,与单纯运脾复方儿宝颗粒对照。
Methods Juvenile rat model of anorexia was established by imitating the major cause of infantile anorexia and treated with Bao Bao Le Liquid . The gastrin β - EP and CCK-8 concentration in hypothalamus , antrum pyloricum and peripheral blood were examined by radioimmunoassay .
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方法采用放射免疫分析技术对健康对照组30例、已确诊的恶性胸水组15例、良性胸水组33例血清及胸水进行CEA、CA50平行对照检测。
Methods The radioimmunoassay technique was used . The CA 50 and CEA level of serum and hydrothorax of 15 patients with malignant hydrothorax , 33 patients with benign hydrothorax , and 30 healthy persons as the control were determined by RIA .
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应用放射免疫分析技术测定人血浆缓激肽(BK),正常值范围:基础1.2~8.3ng/ml,激发0.8~10.3ng/ml,对影响BK因素作了初步分析和讨论。
Plasma bradykinin ( BK ) level in 100 normal subjects is determined using radioimmunoassay . The baseline value is 1.2 ~ 8.3ng/ml and excited value 0.8 ~ 10.3ng/ml respectively . The influential factors of BK are analysed and briefly discussed .
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125-碘标记、双抗体分离的环磷酸鸟苷放射免疫分析技术
125 - iodine-labelled second antibody separation radioimmunoassay for the cyclic GMP
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方法在河南省汉族人群选择180例糖尿病肾病患者;76例健康献血者,用放射免疫分析技术测定了血清瘦素水平。
Methods 180 patients with diabetes mellitus nephron and 76 healthy were measured by radioimmunoassay .
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20世纪80年代出现的时间分辨荧光免疫分析技术,以其独特的优势成为最有发展前途的非放射免疫标记技术。
Time-resolved fluoroimmunoassay technology is a non-radioimmunity labeling technique having most perspective future because of its unique advantage since 1980s .
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采用静态孵育和放射免疫测定技术,研究了生长抑素抑制剂半胱胺盐酸盐对草鱼脑垂体组织单独孵育或下丘脑-脑垂体组织共孵育中生长激素分泌的影响。
Effects of cysteamine hydrochloride ( CSH ), a somatostatin inhibiting agent , on growth hormone ( GH ) secretion from pituitary fragments ( PF ) or hypothalamus pituitary fragments ( HPF ) of juvenile grass carp ( Ctenopharyngodon idellus ) under static incubation conditions were investigated .
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随着放射免疫显像应用技术的进展,放射免疫显像除用于肿瘤的早期诊断外,在其他疾病中的应用也日趋增多。
With its recent development , radioimmunoimaging ( RII ) technique has been used not only for early diagnosing tumor but also for other diseases .