半滑舌鳎

采用生物学信息技术从半滑舌鳎GenBank数据库中下载获得150条DNA序列，利用软件成功设计出106对微卫星引物，经过多态筛选获得65个多态的微卫星位点。

By searching GenBank database of half-smooth tongue sole , 150 sequences were obtained from . PCR primers were designed for 106 loci successfully , of which 65 loci showed polymorphic .

遗传连锁图谱构建：（1）首次利用AFLP标记构建了半滑舌鳎遗传连锁图谱。

The construction of genetic linkage maps ( 1 ) A first consensus AFLP-based genetic linkage map of half-smooth tongue sole ( Cynoglossus semilaevis ) was constructed .

但在半滑舌鳎中，SNP应用研究起步较晚，缺乏足够的SNP位点来构建遗传图谱或进行关联分析。本研究以半滑舌鳎为研究材料，探讨了半滑舌鳎SNP标记的开发及检测。

But there are not sufficient SNP sites for the construction of genetic mapping or associate study owing to the lack of researches on SNPs of half-smooth tongue sole .

研究还发现，在雌性半滑舌鳎繁殖期中，AMH可能具有抑制LHR表达的功能。

Besides , AMH may play a role in suppresses the expression of LHR in Female ovary during reproductive cycle .

在本研究中通过外源激素甲基睾酮（MT）和温度处理半滑舌鳎获得性逆转个体，分析其中性别相关基因的表达变化情况有助于了解性别分化的作用机理。

With the sex reversal obtained by MT immersion and temperature treatment during sex differentiation , it will be helpful to understand the mechanism of sex determination to analyze the sex-related gene expression in sex reversal .

半滑舌鳎（Cynoglossussemilaevis）是一个优良的高效海水养殖品种。

Half-smooth tongue sole ( Cynoglossus semilaevis ) is a rare marine flatfish and one of the most valuable fishes in Chinese coastal .

RT-PCR分析表明该基因主要在卵巢中表达，随着胚胎发育表达逐渐减少，这可能表明该基因在半滑舌鳎卵子形成和早期胚胎发育中起到重要作用。

RT-PCR analysis showed that the gene was highly expressed in ovary and slightly in kidney of female . In different embryo developmental stages , its expression was decline gradually . This result indicated that the survivin may play important role in oogenesis and early embryogenesis .

半滑舌鳎（Cynoglossussemilaevis）是一种重要的经济鱼类，具有生长快等优点。

Half-smooth tongue sole ( Cynoglossus semilaevis ) is an important commercial fish , and has many strong points such as fast-growing in aquaculture .

但养殖半滑舌鳎C19∶1含量为20.41%，明显高于野生半滑舌鳎的含量（11.68%）。

But , the C19 ∶ 1 content in the cultured fish is 20.41 % , obviously higher than 11.68 % in the wild fish .

半滑舌鳎（Cynoglossussemilaevis）是我国一种重要的经济鱼类，它具有肉味鲜美、富含营养、生长迅速等优点。

Half-smooth tongue sole ( Cynoglossus semilaevis ) is an important economic fish in china , which has delicious taste with high nutritional value and grow fast in aquaculture .

半滑舌鳎的孵化腺为单层细胞腺体，起源于外胚层。

The hatching gland with monolayered cells originates from the ectoderm .

石斑鱼和半滑舌鳎封闭循环水养殖系统的构建与运用

Setup and operation of recirculation aquaculture system for Malabar grouper and half-smooth tongue-sole

在所采集的半滑舌鳎疾病样品中，没有发现寄生虫性疾病和病毒性疾病。

There are no viral and parasitic disease fish in our collection samples . 2 .

半滑舌鳎卵子形成中有两种不同的卵黄球生成。

During the development of the oocytes , two kinds of yolk spheres were formed .

雄性半滑舌鳎雄激素受体基因的克隆与表达分析

Cloning and Expression Analysis of Male Half-Smooth Tongue Sole ( Cynoglossus semilaevis ) 's Androgen Receptor Gene

结果表明，半滑舌鳎初孵仔鱼在23℃水温条件下，2&3日龄开口摄食，开口后投喂轮虫。

Results show that larva begin to eat rotifer about 2 & 3 days after hatching at water temperature 23 ℃ .

结果表明，野生与养殖半滑舌鳎的蛋白质含量差异较小，分别为17.20%和17.17%；

As analyzed , the protein contents in the muscles of the wild and cultivated fish were 17.17 % and 17.20 % , respectively .

分析了野生及人工养殖条件下半滑舌鳎背部肌肉的一般营养成分、氨基酸、脂肪酸及常量和微量元素的组成。

Analysis of biochemical composition in the muscles of the wild and cultivated tongue sole ( . Cynoglossus semilaevis . G ü nther ) were conducted .

结果表明，卵黄的吸收是依照先油球、卵黄，后脂肪的顺序被吸收的，半滑舌鳎采用由外向内的吸收方式。

The results showed : The yolk ball absorbs firstly , and then fat ballet , the absorbed way is from outside to inside . 4 .

根据结果表明，在上述孵化条件下，半滑舌鳎的卵子受精后，原生质向动物极集中，油球多分布于植物极。

According to the experiment , the protoplasm centralized to the creatural pole , and the oil globules distributed to the plant pole after the fertilization .

野生与养殖半滑舌鳎的脂肪含量相对较低，分别为0.15%和2.41%。

The lipid contents in the wild and cultivated fish muscles were 0.15 % and 2.41 % , respectively , indicating the fish contains relatively few lipids .

半滑舌鳎是近年来新开发的一种理想的增养殖对象，雌性个体生长速度比雄性快2-3倍。

Half-smooth tongue-sole ( Cynoglossus semilaevis ) is a newly exploited and commercially important cultured marine flatfish , in which females grow 2-3 times faster than males .

脂肪酶的活性在半滑舌鳎早期仔鱼体内一直较低，进入稚鱼期后开始缓慢上升。

The lipase activity kept at a low level in the early larval stage and then increased slowly after entering the juve - ( nile ) stage .

从分类、分布、生态、生理及形态等方面叙述了半滑舌鳎生物学特性，并从生物学的角度介绍了目前研究的现状、存在的问题、研究的方向、发展潜力和发展前景。

The biological characteristics such as classification , distribution , zoology , physiology and morphology were introduced and the actuality of research , problems , developmental potential and prospect was also discussed .

在氨基酸含量方面，养殖半滑舌鳎呈味氨基酸天冬氨酸、谷氨酸、甘氨酸和丙氨酸略低于野生半滑舌鳎。

The contents of taste amino acid , including aspartic acid ( ASP ), glutamic acid ( Glu ), glycine ( Gly ), alanine ( Ala ), in the cultured tongue sole is lower than that in the wild fish .

两者均富含常量和微量元素，其中野生半滑舌鳎的硒和锌含量分别为7.92%和6.29%，较养殖半滑舌鳎高。

Both the wild and cultivated tongue sole are rich in some minerals including rule and trace elements . The muscle of wild tongue sole has higher contents of Se and Zn than the cultured tongue sole , with a ratio of 7.92 % and 6.29 % , respectively .