半抗原

通过ELISA试验、半抗原抑制试验和PCA试验，对头孢菌素抗原决定簇中抗体结合位点进行分析。

Antibody combining sites in an antigenic determinant of cephalosporins were detected by enzyme linked immunosorbent assay ( ELISA ), hapten inhibition and passive cutaneous anaphylaxis ( PCA ) .

通过蛋白质连接技术（戊二醛法）将此半抗原偶联到牛血清白蛋白（BSA）上，制备出全抗原。

The hapten was cross-linked to amino groups on the surface of bovine serum albumin ( BSA ) by glutaraldehyde to obtain whole antigen .

双黄连注射剂中半抗原物质ELISA检测方法的研究

The Study on ELISA Detection Method of the Semi Antigenic Substance of Shuanghuanglian Injection

半抗原Digoxigenin标记HBVdna探针的制备及临床应用

Preparation of HBV DNA Probe Labelled with the Hapten Digoxigenin and its Clinical Application

本实验以克伦特罗（CL）为研究对象，合成了半抗原R&羧甲基醚（CL1）。

R - carboxymethyl ether ( CL_1 ) , a derivative of clenbuterol ( CL ), was synthesized .

用戊二醛将半抗原偶联到牛血清白蛋白（BSA）上，制成全抗原。

The hapten was cross-linked to bovine serum albumin ( BSA ) by glutaraldehyde obtaining antigen .

合成多肽为半抗原制备抗SCF受体单克隆抗体

Generation of mAbs against synthetic SCF receptor peptides

采用戊二醛二步法使辣根过氧化物酶（HRP）与半抗原T4相偶联。

Thyroxine ( T_4 ) was coupled with horseradish Peroxidase ( HRP ) by a two-step linking method of glutaraldehyde .

通过其紫外扫描光谱、SDS-PAGE凝胶电泳试验，表明半抗原和载体成功偶联，免疫动物以后可以产生满意效价。

UV scanning spectrum , SDS-PAGE and animal immunity all indicated that the complete antigens both SMM-BSA and SMM-OVA were successfully produced .

描述了机体对TTX半抗原的抗体反应规律。

The antibody reactive rule against hapten TTX in animal economy was concluded .

用吸收光谱测得每个BSA分子上平均连接33个半抗原。

By using absorption spectrum method , it was detected that about 33 of the GSH-S-DNP haptens were linked to every BSA molecule .

MALDI-TOF-MS法测定芍药苷-人血清白蛋白复合物的半抗原数

Determination of Hapten Number for Paeoniflorin-HSA Conjugates with MALDI-TOF-MS

milbemycinoxime半抗原设计及其抗体制备

Hapten Design and Antibodies Development of Milbemycin Oxime

然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原，采用紫外扫描及SDS-PAGE鉴定。

Then , antigen was prepared by conjugation of hapten with carrier protein by carbodiimide ( EDPC ) method and was identified by UV spectroscopy and SDS-PAGE .

方法将甲霜灵转化成甲霜灵酸，利用适当的方法将其与辣根过氧化物酶（HRP）进行偶联，制备酶标半抗原。

Methods Metalaxyl was acidified to its acid , and then the HRP-Metalaxyl conjugate was prepared by coupling the acid to Horseradish Peroxidase using a water-soluble carbodiimide procedure .

结论：采用人工合成多肽作为半抗原成功地制备出抗β-Netrin的pAb和mAb。

CONCLUSION : Using the synthesized peptides as hapten , we have prepared epitope-specific mAbs and pAb against β - Netrin successfully .

疏水腔修饰法及高活力GPX抗体酶的研究&Ⅱ.半抗原及全抗原的制备和表征

Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity - ⅱ . Synthesis and Characterization of Haptens and Antigens

心钠素Ⅲ（CNⅢ）是一种小分子多肽半抗原。作者以牛血清白蛋白（BSA）为载体，用戊二醛二步法将CNⅢ与BSA进行交联。

Cardionatrin ⅲ ( CN ⅲ ; 24AA ), a small molecular peptide hapten , was cross-linked to bovine serum albumin ( BSA ) by glutaraldehyde two-step conjugation procedure .

本研究分别以牛血清白蛋白（BSA）、卵清蛋白（OVA）为蛋白质载体，采用碳二亚胺法（CDI）将半抗原青霉素钠（PEN）分别与BSA、OVA偶联，合成了完全抗原PEN-EDC-BSA和PEN-EDC-OVA。

In this study penicillin hapten was respectively coupled with BSA and OVA to synthesize PEN-EDC-BSA and PEN-EDC-OVA complete antigen applying CDI .

也表现在B细胞对胸腺依赖抗原SRBC的应答上，以及在T-B细胞协作产生抗半抗原抗体时，T细胞辅助应答也受到抑制的影响。

Antigen SRBC . It also appeared that T-cell supplementary response could become suppressed when B-T cells worked in coordination to produce the antibody against haptens .

结果用碳化二亚胺法成功地将微量半抗原OA与BSA和卵清白蛋白（OVA）制成了完全抗原OA-BSA和OA-OVA。

Results According to carbodiimide method , antigens of OA-BSA and OA-OVA are successfully prepared from minute amount hapten of OA .

用活性酯和混合酸酐法将半抗原2,4-D分别与牛血清白蛋白（BSA）和卵清蛋白（OVA）共价偶联；

Hapten 2,4-D was covalently attached to bovine serum albumin ( BSA ) and ovalbumin ( OVA ) using the modified active ester method and the mixed-anhydride method , respectively .

利用基因重组技术大量生产的任何一种相应半抗原和抗原的抗体片段，与RII技术联合，已应用于临床免疫诊断和科学研究。

The antibody antihalf-antigen and antigenic can be mass-produced by gene recombination and used in RII for clinical immunologic diagnosis and scientific researches .

将氯霉素（CAP）进行化学修饰引入羧基活性基团，合成具有半抗原结构特征的氯霉素半琥珀酸酯（CAP-HS）；

The active group carboxyl was introduced to Chloramphenicol ( CAP ) and formed CAP half succinaate ( CAP-HS ) which has hapten structure by chemical modification .

为建立一种快速检测氯菊酯的酶免疫学方法，以自制的氯菊酯半抗原（Py）与牛血清白蛋白（BSA）偶联得到免疫原。

To establish a kind of competitive ELISA technique for rapid detecting of permethrin , residue Bovine serum albumin ( BSA ) was used to couple with self-made semi-antigen permethrin ( Py ) to obtain immunity source .

以己烯雌酚（DES）、琥珀酸酐和牛血清白蛋白（BSA）等为主要原料，采用混合酸酐反应（氯甲酸异丁酯法）将半抗原己烯雌酚与载体蛋白BSA联接制备成人工免疫原。

Using diethylstilbestrol ( DES ), succinic anhydride and bovine serum albumin ( BSA ) as main stuff , the artificial-immunogen has been obtained by the mixed acid anhydride reaction ( isobutyl chloroformate method ) to connect hapten DES with carrier-protein BSA .

在蒸气浴条件下，T-2毒素与琥珀酸酐（HS）反应合成了T-2HS，薄层层析显示，目标半抗原合成成功；

First , T-2 toxin reacted with succinic anhydride in steam-bath to synthesize T-2 hemisuccinate ( T-2HS ) . Thin layer chromatography analysis showed that the objective hapten was obtained successfully .

本实验以氰戊菊酯为研究对象，从不同的化学途径，合成了两种结构不同的氰戊菊酯半抗原Fen-Hp1、Fen-Hp2。

Two haptens Fen-Hp_1 and Fen-Hp2 with different structure for fenvalerate were synthesized by different chemical pathes .

方法：以P4为半抗原，与血蓝蛋白（LPH）化学偶联制备人工抗原（P4LPH），并以其免疫BALB/c小鼠，制备抗P4的mAb。

METHODS : P4 was coupled chemically with Limulus polyphemus hemocyanin ( LPH ) to form a coupling agent P4 LPH , and then BALB / c mice were immunized with the P4 LPH .

结论TCE经代谢活化后对正常淋巴细胞的毒性增强，促进致敏淋巴细胞的增殖活性，TCE的代谢产物可能作为半抗原诱导了抗原特异性淋巴细胞的增殖转化。

Conclusion Cytotoxicity of TCE to normal lymphocytes and proliferation of sensitized lymphocytes were enhanced by metabolic activation . The metabolites of TCE may act as effective immune hapten to stimulate the proliferation of hapten-specific lymphocytes in TCE-sensitized animals .