刺参

目的观察鱼精蛋白对刺参酸性黏多糖（SJAMP）抗凝血活性的对抗作用及其对SJAMP致死作用、血小板聚集、脾脏增重、抗癌活性的影响。

Objective To observe the effect of protamine ( P ) on biological activities of stichopus japonicus acid mucopolysaccharide ( SJAMP ) .

目的观察大鼠及小鼠肌肉注射刺参酸性粘多糖（SJAMP）的急性毒性反应。

Objective To determine the acute toxicity ( LD 50 ) of SJAMP ( stichopus japonicus acid mucopolysaccharide ) via intramuscular injection on rats and mice .

HPLC指纹图谱技术用于市售刺参质量评价初探

Primary Exploration on Quality Evaluation of Holothurian Sold on the Market by HPLC Fingerprint

结果显示刺参所测组织内无LDH同工酶的表达。

Results showed that there was no expression of LDH isozyme .

E组刺参的吸收效率出现负值，但刺参生长效果明显。

The absorption efficiency of the sea cucumber in group E was minus , but the growth of it was remarkable relatively .

刺参粘多糖及考的松对小鼠移植瘤S180的作用

Effect of SJAMP and Cortisone on Transplanted Mouse S180 Tumor

虽然国内外对海参纲的遗传多样性有些研究，但仍未见有利用DNA分子标记研究仿刺参的遗传多样性相关方面的报道。

Some reports about the genetic diversity of some kinds of Holothuroidea have been seen in some both here and abroad articles but have none about Apostichopus japonicasby by using DNA molecular marks .

刺参初始体重与其后继生长、摄食率和食物转化效率具有密切的正相关性（P0.05）。

The final body weight , food intake and food conversion efficiency for each sea cucumber were generally positively correlated with their initial body weight ( P0.05 ) .

以Wistar大鼠及昆明种小鼠为实验动物，考察了刺参多糖急性毒理作用及安全性。

In this paper , Wistar mice and Kunming mice were used to examine the acute toxicity experiment of Polysaccharide from Stichopus japonicus .

刺参体腔液的渗透压等同于其生活的海水，然而其PH值略低于海水。发现刺参体腔液中存在着细菌。

The osmotic pressure of coelomic fluid of sea cucumber is equal to the sea water they lived ; however , the PH of coelomic fluid is slightly lower than the sea water .

仿刺参体壁、肠和呼吸树全长cDNA文库的构建及部分ESTs初步分析

Construction of cDNA Libraries from Body Wall , Intestine and Respiratory Tree of Sea Cucumber ( Apostichopus japonicus ) and ESTs Analysis

抗病刺参和易感刺参总细胞浓度和透明细胞-淋巴样细胞浓度在第5天差异显著（P0.05）。

In 5th day , the concentration of total cells and the transparent-lymphoid cells of coelomic fluid differed significantly between disease-resistant sea cucumber and disease-susceptible sea cucumber ( P0.05 ) .

目的探讨复方花刺参黏多糖对家兔髂动脉腔内成形术（TA）后血浆内皮素及血清一氧化氮的影响。

Objective To investigate the effects of Stichopus variegatus mucopolysaccharide on plasma nitric oxide ( NO ) and serum endothelin ( ET ) after angioplasty in rabbits .

目的：研究刺参酸性黏多糖（SJAMP）对正常人血小板三磷酸腺苷（ATP）释放的影响，以进一步了解其释放机制。

Objective : To investigate the effect of SJAMP on ATP release of normal platelets and further understand its release mechanism .

结果显示：①抗病刺参和易感刺参体腔液吸光值和颗粒细胞浓度在第10天差异显著（P0.05）。

The results were followed respectively : ① I n 10th day , the absorbency and the granule cells of coelomic fluid differed significantly between disease-resistant sea cucumber and disease-susceptible sea cucumber ( P0.05 ) .

因此，以PVA、SA作为载体添加沸石粉和碳源对菌体进行包埋的技术在刺参池塘养殖的水质和底质环境修复实践中可能具有较大的应用价值。

Therefore , Using the PVA and SA as the carrier and adding zeolite and carbon source for immobilizing bacteria may have greater value in sea cucumber culture pond water quality and sediment environmental restoration practice .

选择不同剂量的刺参粘多糖作用于病毒增殖周期的多个环节，通过观察细胞病变（CPE）、MTT法检测病毒感染后活细胞存量、实时定量PCR检测HSV-1DNA作为评价指标；

Then various concentrations of polysaccharides were applied to different steps of virus replication cycle . Cytopathic effect ( CPE ), methyl thiazolyl tetrazolium ( MTT ) method and real time quantitative PCR were used as evaluation criterion .

采用磁珠富集法分离刺参Apostichopusjaponicus的微卫星分子标记，共获得阳性克隆123个，其中106个含微卫星DNA序列。

In the present study , microsatellites DNA was isolated and enriched from genome of sea cucumber ( Apostichopus japonicus ) by magnetic beads 123 positive clones and 106 microsatellite sequence were obtained .

养殖刺参（Apostichopusjaponicus）溃疡病病原菌致病性研究副猪嗜血杆菌培养特性及致病性研究

Pathogenicity Research of the Pathogen Associated with Ulceration in Cultured Sea Cucumber ( Apostichopus Japonicus ); A Study on Cultural Characteristics and Pathogenicity of Haemophilus parasuis

刺参（Apostichopusjaponicus）是我国传统的名贵海产品，是海参种类中食用和药用价值最高的品种。

Sea cucumber ( Apostichopus japonicus ) is one of Chinese traditional precious seafood species , which has the highest value due to its nutritional and supported medicinal properties among Holothuroidea .

利用AFLP技术，应用96对AFLP引物组合，对刺参雌雄基因组DNA进行检测，筛选与刺参性别相关的分子标记。

AFLP technique was used to screen the sex-related DNA markers through analyzing DNA differences between male and female of sea cucumber ( Apostichopus japonicus ) . 96 pairs of AFLP primer were selected to detect the genome DNA of the male and female individuals by AFLP analysis .

结论家兔髂动脉TA后ET增多，NO减少，存在血管内皮功能失调，而复方花刺参黏多糖通过调整ET和NO的平衡改善血管内皮功能。

Conclusion After iliac arterial TA the concentrations of plasm ET were increased and the serum NO were decreased , the endothelial function fell into disorder . The Stichopus variegatus mucopolysaccharide had the effects of improving the endothelial function after angioplasty in rabbits by adjustment of plasm ET and serum NO.

使用CILAS940L型激光粒度仪对刺参肠道内含物及自然沉积物的粒度进行分析。

Particle sizes of the gut contents and the natural sediments were analyzed and compared by using CILAS940L Laser Particle Size Analyzer .

水质分析结果表明，刺参密度为15ind·m-2时底泥总氮、总磷含量降幅最大。

The analysis of water quality showed that the content of total nitrogen and total phosphorus in mud had the largest decline when the density of A. Japonicus was 15ind · m-2 .

仿刺参幼体烂胃病及其致病原鉴定从患烂胃病的耳状幼体中分离到1株细菌LW-1，人工回接感染实验证实其具有较强的致病性，并与自然发病症状相同。

One dominant bacterium LW-1 was often isolated from the affected auricularia , and it was verified to be the pathogen causing the stomach ulcer disease through an artificial infection test .

Vangieson染色法观察体壁组织结构，发现胶原纤维和肌原纤维分布在仿刺参体壁的不同位置，其中大部分为胶原纤维，仅含少量肌原纤维。

Using Van Gieson staining technology to observe the structure of the different part of raw sea cucumber . The results showed that collagen fibrils and myofibrils were distributed in different part of S. japonicus body wall among which the most parts were collagen fibrils . 2 .

分析了不同产地的10批仿刺参样品，采用国家药典委员会推荐的中药色谱指纹图谱相似度评价系统（2004A版）处理谱图，确定了6个共有峰。

Ten different original samples are analyzed , and 6 peaks are identified as common fingerprint peaks using the similarity evaluation system for chromatographic fingerprint of TCM ( Version 2004 A ) recommended by State Pharmacopoeia Committee of China .

刺参控温促熟及人工育苗试验铝合金熔化炉炉膛控温试验及应用

Test and Application of Chamber TemperatureControl of Aluminum Alloy Melting Furnace

刺参酸性粘多糖对人血小板超微结构的影响

The Effects of Stichopus Japonicus Acidic Mucopolysaccharide on Human Platelet Ultrastructure

在中国刺参养殖已经成为一个快速发展的水产业。

Its farming becomes a fast growing aquaculture industry in China .