传代培养

结果从SD大鼠胚胎前脑皮质分离的组织经原代和传代培养均可形成细胞克隆，并具有增殖的能力。

Results : Cell clones could from the forebrain cortices of embryonic rat after primary and passage cultivation .

采用RTPCR法从牛海马组织中扩增牛m1和m5受体内环3区，并通过半定量RTPCR法检测两种受体mRNA在传代培养前后的牛主动脉内皮细胞中的表达。

These two receptors mRNA expressed in the bovine aortic endothelium were assayed by semi_quantitative RT_PCR .

结果显示：Ca2＋浓度以及成骨细胞传代培养的代数是影响酶活性的重要因素。

Ca 2 + concentration and osteoblast culture generation were important factors affecting ATPase activities .

从原始生殖细胞分离昆明小鼠EG细胞及其传代培养

Isolation of Kunming Mice Embryonic Germ Cells Derived From Primordial Germ Cells and Their Passage Culture

结果HTC在传代培养中显示稳定的增殖活性并分泌II型胶原。

Results The HTC proliferated stably in monolayer culture with positive expression of collagen type II .

用胶原酶灌注消化，收集小牛主动脉内皮细胞（EC）进行传代培养。

Arterial endothelial cells were obtained from bovine aorta by mild treatment with collagenase and medium perfusion .

密度梯度离心法获得大鼠MSCs，传代培养并扩增。

MSCs were obtained from SD rats using the method of density gradient centrifugation culture .

传代培养前后内皮细胞差异表达基因消减cDNA文库的构建

Construction of a subtracted cDNA library of differentially expressed genes in freshly isolated adult and cultured new-born bovine aortic endothelial cells

结论用抑制消减杂交法构建的传代培养前后主动脉内皮细胞差异表达基因消减cDNA文库富含动脉粥样硬化相关基因。

Conclusion The subtracted cDNA library constructed by SSH technique contains the atherosclerosis related genes when the aortic endothelium were cultured .

经原代培养及传代培养后获得nestin阳性细胞。

The nestin positive cells were harvested through primary culture and subculture .

采用酶消化法从初生2天SD大鼠头盖骨中分离出成骨细胞，用含药血清及生理盐水进行成骨细胞传代培养；

Then the TCD-containing serums were extracted ; osteoblasts were separated from the skull of two-day-old SD rat by enzyme digestion ;

本文以传代培养的人胚肺成纤维细胞为研究对象，以Fe~（2+）/维生素C为诱发脂质过氧化的体系，研究了脂质过氧化对细胞衰老的影响。

Fe2 + / vitamin C system was used to stimulate lipid peroxidation in human di-ploid fibroblasts derived from embryonic lung tissue to study the relationship between lipid peroxidation and cell senescence .

结果：原代及传代培养显示，兔BMSCs具有活跃的增殖能力。

Results : The BMSCs of rabbit showed active proliferative capability in primary and passage cultures .

与HIV-1共感染的HHV-6毒株的分离、传代培养与鉴定

Isolation , passage and identification of HHV-6 co-infected with HIV-1

实验选取清洁级SD新生鼠10只，无菌条件下分离脑组织，克隆传代培养脑源性神经干细胞。

Ten newly born SD rats of clean grade were selected to isolate the brain tissue under sterile condition and clone brain-derived NSCs .

传代培养的NSC性能稳定；

The property of NSC was stable after long culture and preservation .

方法：采用酶消化法从新生SD大鼠头盖骨分离成骨细胞，传代培养。

METHODS : Using the method of enzyme digested , Osteoblasts ( OB ) obtained from newborn rat calvaria were cultured for serial passage .

另外研究血管内皮生长因子（VEGF）和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。

In addition , we tested the effect of vessel endothelial growth factor ( VEGF ) and passage on cell differentiation , expansion kinetics and apoptosis .

方法：1.新生Wistar大鼠肺成纤维细胞原代及传代培养，第四代细胞用于实验研究。

Pulmonary fibroblasts from newborn Wistar rat were isolated in primary generation and fourth generation of cells was used for experiments . 2 .

说明其潜伏期<24h，传代培养对数增殖期为2～5d，对数增殖期结束后，第6天细胞生长缓慢，进入平台期。

After log proliferation period , the growth of cells entered the platform period .

在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。

The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture .

传代培养PC12细胞的早期细胞分裂过程观察

Cell fragmentation in subculture of PC 12 cell line

方法：将抽取的兔子骨髓用密度梯度离心和贴壁培养法分离bMSCs，在体外进行细胞的原代和传代培养，观察细胞的生长情况。

Methods : bMSCs were extracted from the bone marrow in rabbits by density gradient centrifugation method and cultured in vitro regularly .

方法：胃癌BGC-823细胞传代培养。

Methods : Gastric carcinoma BGC-823 cells were cultured for serial passage and then treated with bFGF .

目的建立一种无血清传代培养黑素细胞的方法，并观察抗角蛋白自身抗体（AKautoAb）对无血清培养黑素细胞及角质形成细胞增殖的影响。

AIM To observe the effect of anti keratin autoantibody ( AK auto Ab ) on proliferation of melanocytes and keratinocytes cultured with serum free medium .

结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。

Results The area positive for ALP staining is no less than 90 % in the primary culture and almost 100 % in the subculture .

方法对着色性干皮病患者成纤维细胞进行传代培养、建株，采用放射自显影和细胞融合技术分析XP细胞互补组。

Methods From four XP patients , skin fibroblast cell strains were derived and used for the complementation group analysis by autoradiography and cell fusion technique .

Wistar鼠主动脉内皮细胞传代培养中6-酮-PGF（1α）和TXB2水平改变的年龄关系

Aging Relation to the Changes of 6-keto - PGF_1a and TXB_2 Levels in Cultivated Fluids of Endothelial Cells of Thoracic Aortae in Wistar Rats

贴壁24h后去悬浮细胞、低接种密度传代培养可获增殖能力强、功能活跃的MSCs。

Discarding the suspending cells after 24h incubation and subculturing the cells with low seeding density were helpful for acquiring MSCs with improved reproductive capability and active function .

ADSCs体外扩增和自我更新能力很强，传代培养易于获得大量有分化能力的细胞。

ADSCs have strong ability to self-renewal and mass culture accessible to a large number of cell differentiation in vitro .