细胞培养基

动物细胞培养基的发展及应用

Development and application of animal cell culture medium

目的：动物细胞培养基是体外细胞培养的重要因素，能够影响细胞生长。

OBJECTIVE : Animal cell culture medium is an important factor of cell culture in vitro , which can influence the cell growth .

无牛血清IgG细胞培养基（-GFCS培养基）的制备及其在杂交瘤细胞体外培养中的应用

Preparation and application of-GFCS medium to culturing hybridoma cells in vitro

应用ELISA检测间质细胞培养基上清液中VEGF浓度？

The concentration of VEGF in the supernatant of the media was assayed by ELISA .

SDS-PAGE显示纳米颗粒分散在含血清的细胞培养基中，其表面将吸附蛋白质。

In cell culture medium with serum , proteins bound to the surface of targeting nanoparticles analyzed by SDS-PAGE .

第4组：10ng/mlbFGF预处理一天，再加不含血清的改良的神经细胞培养基（Modifiedneuronalmedium，MNM）处理；

Group 4 : 10ng / ml bFGF pretreated MSCs for a day , followed with modified neuronal medium and serum free ;

结论兔及SD大鼠的胰腺基质细胞培养基均可将兔MSCs诱导分化为IPCs。

Conclusions Rabbit MSCs can be induced into IPCs by post-culture medium of rabbit and SD rat PSCs .

通过测定释放至细胞培养基中的乳酸盐脱氢酶（LDH）活性。

Effect of allopregnanolone or GABA on the excitotoxicity was examined by the measurement of lactate dehydrogenase ( LDH ) activity released in the culture medium .

方法：制备4种牙体修复材料的细胞培养基（DMEM）浸出液，采用四唑盐MTT比色分析法测定材料对人牙髓细胞生长的影响；

METHODS : 4 dental restorative materials were soaked in culture medium ( DMEM ) for leachate solution . The effect of each material on viability of pulp cell was measured by the MTT assay .

收集瞬时转染及筛选后的细胞培养基，用酶连结免疫吸附分析（ELISA）检测试剂盒检测人B型利钠肽（hBNP）的表达水平。

After transfected and during selected , the concentration of human B-type natriuretic peptide ( hBNP ) in cell culture medium was detected by enzyme-link immunosorbent assay ( ELISA ) .

RH-CM也可作为小鼠ES细胞培养基的添加物，用含70%RH-CM的ES细胞培养基和小鼠胚胎原代成纤维细胞饲养层（PMEF）培养ES细胞，可长期有效地维持其未分化状态和二倍体核型。

RH-CM can be used as supplement of ES cell media . ES cells which are cultured in media containing 70 % RH-CM and on PMEF feeder can maintain their undifferentiation state and diploid karyotype .

分别在细胞培养基中添加5%、10%、15%、20%及25%新生牛血清（newbovineserum，NBS）培养ECs，比较不同浓度NBS对ECs体外增殖的影响。

The ECs were cultured in the medium supplemented with 5 % , 10 % , 15 % , 20 % or 25 % new bovine serum ( NBS ) . The effects of the different concentrate NBS on the in vitro proliferation of the ECs were compared .

结果高效液相检测神经干细胞培养基未测到单胺类生物活性物质，而经体外培养增殖10d、14d、30d的细胞培养基可检测到去甲肾上腺素（NE）和多巴胺（DA）。

Results No monoamine biological activity materials in nerve stem cells culture medium were detected . However , the contents of Noradrenaline ( NE ) and Dopamine ( DA ) were detected in the culture mediums which were cultured for 10 , 14 and 30 days by HPLC .

Gonda教授说：“时间会告诉我们的。但是，我们必须扩大研究，看看在实验室乳腺癌细胞培养基中的结果是否会在动物上重现”。

" Time will tell * but we have to expand these studies to show that what we 've seen in laboratory cultures of breast cancer cells is also true in experiments on animals ," Prof Gonda said .

不同细胞培养基细胞培养动力学比较试验

Comparative Trails of Dynamics of Cell Culture in Different Cell Media

无血清细胞培养基添加蛋白&白蛋白和转铁蛋白的制备和纯化

Preparation and Purification of BSA and Transferrin Used for Serum-free Cell Culture Media

细胞培养基的吸收光谱研究

The Study of Absorption Spectrum for Cell Substrate

可是，生物化学家已完成了在无细胞培养基上增殖过程的某些阶段。

However biochemists have achieved certain steps of the multiplication process in cell-free media .

动物细胞培养基的开发与优化是动物细胞培养中的重要环节之一。

For animal cell culture , developing culture medium represents one of the most important steps .

骨髓基质细胞培养基对体外脊髓运动神经元的影响

The influence of culture fluid of bone marrow stromal cells on spinal cord motor neuron in vitro

还可检测出对病毒的抗体，在细胞培养基上可以分离出病毒。

Antibodies to the virus can be detected , and the virus can be isolated in cell culture .

通过改变细胞培养基的组成和培养条件，可以有效提高齐墩果酸的产出率。

The extracting amount of the compound was improved by changing the composition of the culture medium and culturing conditions .

胰酶分次消化及无血清角化细胞培养基培养甲床上皮细胞的体外实验

Nail bed epithelia in vitro cultured with the method of enzyme digestion by different times in serum-free medium containing keratinocytes

第一种采用给家兔灌服血府逐瘀浓缩丸后取其血清加至细胞培养基中进行实验即血清药理学方法；第二种直接加血府逐瘀煎剂浓缩液至培养基中。

The first experiment approach adopted the addition of the rabbit 's serum to the culture medium while in the second experiment the CXZP was added to the medium directly .

多数是在鸡胚中生产的灭活病毒疫苗，一些是在细胞培养基中生产的灭活疫苗，还有几个减活病毒疫苗。

Most will be inactivated virus vaccines made in eggs , some will be killed virus vaccines made in cell cultures and a few will be live attenuated virus vaccines .

通过调节培养的鼠表皮角质形成细胞培养基中钙离子浓度，在不同时间段应用免疫细胞化学检测C/EBPα和C/EBPβ的表达情况。

The expressions of C / EBP α and C / EBP β were tested with immunocytochemical method at different periods by adjusting the concentration of calcium ions in the culture medium of epidermal keratinocyte in mice .

结论利用5%胎牛血清的人内皮细胞培养基，添加生长因子和胰岛素-转铁蛋白-硒添加物，并用纤维连接蛋白包被培养瓶，能简单有效地培养人视网膜血管内皮细胞。

Conclusion Media of HE SFM BGM contained 5 % FBS and ITS ,β ECGF can culture purified human retinal capillary endothelial cell . Fibronectin coated dishes and growth factors addition are essential for the culture of endothelial cell .

摘要综述了昆虫细胞培养基的现状和发展、无血清培养基的开发、昆虫细胞系的建立以及昆虫细胞在生物农药和重组蛋白中的应用。

Development of insect cell culture media and serum-free media , establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper .

大鼠心脏细胞条件培养基对小鼠ES细胞特性的维持

The Medium Conditioned Incubation with Rat Heart Cells Maintains the Properties of ES Cells

~（60）Coγ射线照射K562细胞后培养基转移介导的旁效应研究

Bystander effect of K562 cells by medium transfer experiment after ~ ( 60 ) co γ - rays irradiation