细胞破碎

采用亲和层析的方法对细胞破碎液进行纯化，通过SDS-PAGE电泳分析，在电泳图上获得一条纯化后的单一条带。

Affinity chromatography using the method of cell disruption liquid was purified by SDS-PAGE electrophoresis analysis , gel electrophoresis obtained after purification of a single band .

发状念珠藻不同细胞破碎方法的研究

Comparison of Different Cell Disruption Methods for Nostoc Flagelliforme

然后通过超声波细胞破碎获得胞内可溶性蛋白，利用镍柱亲和层析法纯化得到电泳纯的重组NTD。

The recombinant NTD was purified from intracellular proteins through sonication and Ni-affinity chromatography .

其主要问题是总酶活相对较低，且胞外CAT产量不高，经细胞破碎获取胞内酶成本较高，不适合工业化生产的应用。

However , the major problem was that the total enzyme activity was not very high and the extracellular CAT production was low , requiring great cost for cell rupture in order to obtain the intracellular enzyme .

结果如下：（1）在OA注射中心区神经元早期出现胞体固缩、肿胀、核移位，在注射3d时细胞破碎，发生坏死，并有大量炎性细胞浸润等病理现象；

The results showed that ( 1 ) the neurons in the center of the injection region displayed cytoplasmic shrinkage , swelling , nuclear pyknosis , and dislocation at the early stage , and necrosis appeared 3 d after the injection .

在IPTG诱导下，两个表达菌株的无细胞破碎液均具有激发烟草叶片过敏反应（HR）的活性。

After induced by IPTG , the Cell Free Crude Solution (( CFCS )) of ( hrf 1 ) and ( hrf 2 ) can elicit hypersensitive reaction ( HR ) on tobacco leaves .

试验发现Hib菌体细胞破碎溶解产物对小鼠的非特异性免疫功能无明显影响，而对小鼠的特异性免疫功能有较为显著的影响。

The result indicated that Hib bacterial extract had no significant effect on the non-specific immune but had more significant effect on specific immune function in mice .

对含藻水及藻细胞破碎条件下水样中有机物的分子质量分布、吸光度（UV）、总有机碳（TOC）进行了研究。

Under the conditions of algae contained water and algae cell crush , a study was carried on the molecular weight ( MW ) distribution of organic matter , absorbance ( UV ) and total organic carbon ( TOC ) of the water sample .

在设定超声波的占空比为50%、输出功率为150W、破碎时间为20min的条件下，紫球藻细胞破碎率可达87.4%，高于反复冻融法最高可达到的72.2%。

When duty cycle , output power and crushing time respectively are 50 % , 150W and 20 minutes , the cell fragmentation efficiency arrives to 87.4 % .

以金黄色葡萄球菌（S.aureus）为指示菌，采用琼脂扩散法检测细胞破碎率对抑菌活性的影响，抗菌活性表明：细胞充分破碎，有利于活性物质的释放和提取。

And the results of antimicrobial activity , which determined against S.aureus using agar diffusion method , indicated that the sufficient crushing efficiency of alga cells was propitious to releasing and distilling of active substances .

离心收集处于生长平衡后期的螺旋藻（Spirulinamaxima），通过渗透冲击协同冻融细胞破碎、冷水提取，TCA除蛋白、乙醇沉淀得到粗多糖。

Centrifuging and harvesting Spirulina maxima at the stationary phase , and fragmentizing cells by penetrated and frozen-melt , then extracted by cold water , removing proteins by TCA , at last , the raw polysaccharide was deposited by ethanol .

本文着重考察了搅拌粗提、珠磨破碎、高压细胞破碎仪和高压破碎仪的细胞破碎方法对释放ALDH的影响。

The stirring , the glass bead breaker , the high pressure cell cracker and the high pressure cracker to break cell for releasing ALDH were investigated and compared .

结果表明，用高压破碎仪破碎，酵母细胞破碎率可达到95%以上，而且有效地防止了ALDH的分解及降解，是一种较为理想的破碎方法。

The results showed that cell-breaking by high press cracker was the best methord with its breaking rate above 95 % and it could effectively prevent ALDH decomposition and degradation .

本文着重考察了自溶、反复冻融、超声波和高压破碎仪的细胞破碎方法对释放ALDH的影响。

The effects of different cell-breaking methods including self-dissolving , repetitive freezing and thawing and ultrasonication and high pressure cracker on ALDH release were studied and compared in this paper .

为研究从螺旋藻中分离纯化SOD的最优化方法，采用细胞破碎方法、最佳丙酮用量、最佳硫酸铵饱和度和DEAE-52离子交换层析技术分离纯化螺旋藻中的SOD并作比较分析。

S In order to study the optimal methods for isolate and purify SOD from Spirulina platensis , cell-broken methods , the optimal amount of acetone , the optimal saturation of ammonium sulfate and the conditions of DEAE-52 iron exchange chromatography were adopted and compared .

实验得出，红细胞破碎的最佳条件为：超声波破碎功率为200W，时间为10min，红细胞与加入水的比例为5∶1。

Experiments indicated the optimum conditions of disruption of the red cell : the power is 200W , time is 10 min , V_ ( red cell ) ∶ V_ ( water ) is 5 ∶ 1 .

经过细胞破碎仪处理两次（80MPa）后，样品（20mg干重/mL）中的细胞可被毛地黄皂甙（3mg/mL）有效破碎，细胞质膜即可通过蔗糖密度梯度离心得以分离。

After passed twice through French pressure cell ( at 80 MPa ), cells in sample ( 20 mg of dry weight / mL ) were disrupted effectively by digitonin ( 3 mg / mL ), and then the cytoplasmic membrane was isolated by density gradient centrifugation .

用细胞破碎与两水相萃取相结合的新工艺从废弃的啤酒酵母细胞中提取乙醇脱氢酶，探讨了影响破碎萃取效率的因素，确定了破碎萃取的最佳工艺条件

The new technology that aqueous two-phase extraction and cell disruption

纳米级微生物细胞破碎机研制及应用

Manufacture and application of nanometer grade microorganism cell crushing machine

紫球藻细胞破碎方法研究

Research on the Methods of Porphyridium cruentum Cells Fragmentation

氯化血红素提取中超声波细胞破碎条件的研究

Study on using supersonic wave to disrupt red cell in extraction of hemin

珠磨匀浆细胞破碎技术

Technology of Refining Cell Disruption with Bead Grinding

抗氧化剂对根瘤土壤杆菌细胞破碎的研究

Study on optimum conditions of cell disruption of the Agrobacterium tumefaciens strain with antioxidant

细胞破碎对离子交换扩张床吸附中生物质与吸附剂间相互作用的影响

Influence of cell homogenization on interactions between biomass and adsorbent in expanded bed ion-exchange adsorption

与顺铂阳性对照组相比，细胞破碎现象不明显。

Compared with positive control group of Cisplatin injection , cell fragmentation was not obviously .

将修饰菌用于牛血红细胞破碎液中牛血红蛋白的分离纯化，富集倍数为10.0。

Modified biomass for the purification of bovine blood samples , enrichment fold were 10.0 .

运动训练引起自由基代谢的紊乱是导致红细胞破碎增加的主要原因，也是导致运动性低血色素和运动性贫血的重要因素。

Exercise-induced disturbance of free radicals metabolism is the main reason of the increase of erythrorrhexis .

铸造用砂模成形机（不包括喷砂装置）冲击式细胞破碎装置

Forming machine for making foundry moulds of sand ( excl. sand-jet sprayers ) impact cell mill

通过对反复冻融法和超声波细胞破碎方法进行比较得知，后者对紫球藻细胞的破碎效率要高于前者。

Compared with the method of freezing and thawing , the ultrasonic method has higher crushing efficiency .

探索了将膨胀床吸附层析应用于动物血液蛋白质的提取，尝试了直接从牛血红细胞破碎液中纯化血红蛋白。

Direct separation and purification of hemoglobin from fresh bovine hemolysate were studied with expanded bed adsorption .