非病毒载体

细胞特异性的非病毒载体增强BMP-2在成纤维细胞中表达

Cell Specific Non-Viral Vector Enhances Expression of BMP-2 Gene in Human Dermal Fibroblasts

非病毒载体介导Mn-SOD基因在小鼠肝脏的高表达

Non-viral vector-mediated high efficiency expression of manganese superoxide dismutase gene in mouse liver

目前常用的基因治疗载体系统有逆转录病毒、腺病毒、腺相关病毒等病毒载体，以及脂质体、裸DNA、各种微粒或聚合物包裹等非病毒载体系统。

The staple carrier system includes viral carriers such as retrovirus , adenovirus , gland-related virus and non-viral-carriers such as lipidosome , bare DNA or diversified particulates . The early gene therapy studies used to make retrovirus as a carrier .

结论LPD是一种制备工艺简单、体外稳定性好、转染率高，具有应用潜力的非病毒载体系统。

Conclusion LPD could be prepared easily with small particle sizes and high transfection activities . LPD may be good non-viral vectors for applications in gene delivery .

非病毒载体介导的人凝血因子Ⅷ基因在小鼠32D细胞系中的表达

Non Viral Vector Mediating Human Coagulation Factor ⅷ Gene Expression in Mouse 32D Cell Line

目的：寻找能高效转染永生化神经前体细胞（IN-PC）的非病毒载体，观察转染后增强型绿色荧光蛋白（EGFP）的表达。

AIM : To investigate an effective transfection method of non-viral vectors and the expression of enhanced green fluorescent protein ( EGFP ) in the transfected immortalized neural progenitor cells ( INPC ) .

结论：PEI是一种高效、有用的非病毒载体，能够在培养的哺乳动物细胞中进行基因转移，这对疾病的基因治疗具有潜在的应用价值。

Conclusion : The LMW-PEI described here was a new , highly efficient , and non-cytotoxic vector with a favorable efficiency for gene therapeutic applications . Primary culture studies have shown that exogenous NGF induces differentiation of NB cells depending their survival on NGF .

目的：为提高非病毒载体转染细胞后的基因表达水平并延长表达时间，并为骨髓间充质干细胞（MSC）生长创造更好的环境，以期用于软骨损伤的体内再生治疗。

Objective : To enhance the level and prolong the duration of gene expression for gene-engineered rat mesenchymal stem cells ( MSCs ) using non-viral vector , and to provide a better environment for the proliferation of MSCs for the clinical therapy of cartilage defect . Methods : 1 .

目前的基因传递系统主要包括病毒载体和非病毒载体两大类。

Current gene delivery systems include viral vectors and non-viral delivery systems .

非病毒载体与病毒载体相比具有无免疫原性，无遗传毒性等优势。

Compared with viral vector , non-viral vector was non-immunogenicity and non-genetoxic .

非病毒载体介导神经干细胞基因转染的效果观察

Transfection efficiency of neural stem cells mediated by non-virus vector

结论：非病毒载体纳米微囊-VEGF复合体能明显促进慢性创面愈合。

Conclusion Non-virus nanosphere-mediated VEGF could obviously promote the healing of chronic wound .

目前广泛应用的病毒及非病毒载体不能很好地满足临床要求，是基因治疗亟需解决的关键技术。

The widely used gene therapy vectors nowadays can not meet the demands .

基因治疗中关于非病毒载体的最新设计策略

The Advances in Non viral Techniques for Gene Transfer

非病毒载体负载增强型绿色荧光蛋白转染永生化神经前体细胞

Transfection of enhanced green fluorescent protein into immortalized neural progenitor cells by non-viral vectors

两者均为基因治疗提供具有潜力的新型非病毒载体。

Both types of non-viral gene vector had potential ability used for gene therapy .

因此寻找新的非病毒载体成为目前的研究热点。

The recent topic has been focused on the new non - viral vectors .

目的探讨和构建一种用于靶向性基因治疗的非病毒载体。

Objective To construct a non - viral vector for targeting cancer gene therapy .

三种非病毒载体转染方法的比较

Comparison of three Non-viral vector for gene transfection

基因传递常用的载体一般分为病毒载体和非病毒载体。

The vectors of gene transfection can be divided into viral vectors and non-viral vectors .

非病毒载体融合蛋白基因的克隆、表达及表达产物的纯化和功能鉴定

Cloning , Expression and Purification of Fusion Protein Genes as Well as Their Function Research

目前非病毒载体的研究已不局限于阳离子聚合物材料，其他的多种材料，如：无机化合物都成为了研究对象。

The study of non-viral gene vectors has not been limited in the polymeric cations .

然而，目前应用的非病毒载体，如多聚阳离子聚合物和脂质体，存在着转染效率低，尤其在体内的转染效率低等缺点。

But most non-viral vectors are limited in low efficiency of transfection , especially in vivo .

非病毒载体尽管安全性较高，但其效率较低，离实际应用的要求相差甚远。

Non-viral vectors are generally safer than viral approaches , but their efficiency is much lower .

一种非病毒载体的组成与体外转基因能力的研究

Study on the Composition of Copolymer and Its Gene Delivery as a Non-viral Vector in vitro

非病毒载体介导的基因转染效率及其对人舌鳞癌细胞生长增殖的影响

Efficiencies of Several Non-viral Vectors-mediated Gene Transfection and Their Influence on Tongue Squamous Carcinoma Cells In Vitro

由于病毒载体安全性的局限，现阶段的基因治疗主要研究非病毒载体。

With the limit of viral vector 's insecurity , present gene therapy are mainly about non-viral vector .

基因载体包括病毒载体和非病毒载体，目前基因治疗中使用的主要是病毒载体。

Gene vectors include viral vectors and non-viral vectors , while viral vectors are mostly applied in clinic .

壳聚糖作为一种天然生物多糖物质，已被证明是具有潜在前景的非病毒载体材料。

Chitosan is the natural biological polysaccharide which has been proven to be the potentially promising non-viral vector materials .

基因转移载体是基因治疗的重要组成部分，包括病毒载体和非病毒载体。

Gene transfer vector is the important ingredient in gene therapy , which includes virus vector and non-virus vector .