蛋白质沉淀

样品制备在HPLC的生物样品分析中是关键性的一步，经典的样品预处理包括蛋白质沉淀和药物萃取。

( 7 ) The sample pretreatment is the key step in the biological sample analysis by HPLC . The sample pretreatment contains the protein precipitation and the analyte extraction , thus the operation is relatively laborious and time-consuming .

结合近年发表的文献具体阐述了固相萃取法、蛋白质沉淀法、超滤离心法、亲和层析法和部分其他方法的原理及其应用实例。

It also introduces the principles and applications of variety of sample preparation methods including solid phase extraction , protein precipitation , ultra-filtered centrifugation and affinity chromatography .

这一结果也表明，我们实验装置的可操作性，以及SAS过程用于水溶液为主体混合溶剂中蛋白质沉淀的可行性。

The results also demonstrated that our experimental apparatus are operable and SAS process is feasibility to precipitate proteins in aqueous mixed solvent .

作者用硫代巴比妥酸（TBA）呈色，经对试剂进行调整，不使蛋白质沉淀直接测定血清（浆）过氧化脂质（LPO）。

A method for determination of lipid peroxides ( LPO ) was established with thiobarbituric acid reaction without precipitating protein .

目的：分析比较生理盐水（NS）与护理液对软性隐形眼镜蛋白质沉淀静态洗脱效应。

Objective : To evaluate the cleaning effectiveness of normal saline and multi purpose care solutions on hydrophilic contact lenses when protein deposits are extracted without rubbing .

由于该饮料不稳定而引起脂肪上浮和蛋白质沉淀，使产品外观和口感欠佳，针对此问题，对不同HLB值的混合乳化剂和稳定剂的效果进行实验。

The method claims to counteract the problems caused by unstable acidic media in this type drink , such as fat separation and sediment protein , and consequent poor appearance and mouthfeel .

方法应用层析介质及蛋白质沉淀技术从螨代谢分泌物中分离、纯化粉尘螨变应原DffF3及国际公认的变应原DerfⅢ，经临床检测和放射性变应原吸附实验（RAST）鉴定其变应原性。

Methods The allergenic proteins , Dff F3 and Der f ⅲ ( well known allergen ) from the whole culture extract of Dermatophagoides farinae were purified by chromatography and sedimentation techniques . Allergic activities of both proteins were identified by skin prick test and RAST .

利用蛋白质沉淀光度法测定黄芩和茶多酚对于主要致臭底物蛋白质的沉淀能力；

Protein precipitation-spectrophotometry was used to evaluate the ability of depositing protein ;

样品采用柠檬酸为蛋白质沉淀剂，高效阴离子交换色谱法分析测定。

Citric acid was used for the precipitator of samples .

蛋白质沉淀结晶研究进展

Research Progress in the Precipitation and Crystallization of Proteins

软性角膜接触镜片上蛋白质沉淀洗脱的动态观察

Dynamics of protein deposits on hydrophilic contact lenses

摘要目的：研究市售的软镜多功能护理系统对镜片上蛋白质沉淀的清洗效果与动力学。

Objective : To investigate the cleaning efficacy and dynamics of multi-solution on proteins deposited on hydrophilic contact lenses .

目的：评价共焦点显微镜对亲水性软性角膜接触镜片上蛋白质沉淀情况的观察作用。

Objective : To evaluate the value of the confocal microscope in observing protein deposits on the hydrophilic contact lens .

伴随错误折叠的蛋白质沉淀，一些机制和下游事件有牵连，包括细胞保护作用的破坏。

Several mechanisms have been implicated in the down-stream events following deposition of misfolded proteins including failure of cellular defenses among many others .

例如，严重脱水会造成晶状蛋白质沉淀，促使细胞碎成一堆，这便是白内障。

For example , severe dehydration can cause crystallin proteins to precipitate , prompting their cells to crumble into a clump - a cataract .

酒体中的多酚（单宁）、金属离子（如铁）、乙醇也会引起蛋白质沉淀。

Besides , the polyphenol such as tannin , metal ions such as Fe , and ethanol would also induce protein precipitate in yellow rice wine .

用途：在食品工业中作水产调味的组织改进剂、金属螯合剂、脂肪乳化剂、保湿剂、水的软化剂、蛋白质沉淀剂。

Uses : Used as a fatter emulsifier , enhance water holding capacity , water soften agent , chelating agent and texture modifier mainly used in seafood flavors .

黄酒产品在货架期容易发生非生物浑浊和沉淀，浑浊沉淀形成的机理主要有蛋白质沉淀、铁沉淀和氧化沉淀。

Non-biological turbidity and precipitate easily occurred in yellow rice wine on shelf . The mechanism of precipitate formation mainly covered protein precipitate , iron precipitate and oxidation precipitate .

可采取在麦汁煮沸阶段加入胶体物质和在贮酒过程中加入硅胶，使啤酒中的高分子蛋白质沉淀而过滤除去。

Addition of colloid substances during wort boiling process and addition of silica gel during beer storage could make high molecular proteins in beer form precipitate and the precipitate could be removed by filtration .

在经过蛋白质沉淀、加热、离心、脱色、过滤和水解等物理-化学法处理之后利用葡萄糖氧化酶将马铃薯淀粉废水进行生物酶降解反应。

After a series of physical-chemical treatment which included protein precipitation , heating , centrifugation , decolorization , filtration and hydrolysis , the waste water was further treated by using biological enzyme Glucose Oxidase .

蛋白质沉淀和二氧化硫漂白分析研究得出，葡萄汁和葡萄酒中的单宁酸和聚合色素有相同的结论。

Tannic acid in grape juice and in grape wine ( also analyzed by protein precipitate analysis method and traditional sulfur dioxide bleaching analysis method ) had almost the same rules as polymeric pigments did .

介绍了啤酒中蛋白质沉淀形成的机理，蛋白质稳定技巧，硅胶的种类、特性及其在啤酒稳定方面的应用。

The machanism of the formation of the protein precipitation in beer , the stability of the protein , the type and the property of the silicagel and its use in the stability of beer were stated .

脱蛋白过程中使蛋白质沉淀的机理可能是造成多糖损失的主要原因，蛋白酶解法脱蛋白效果最好且多糖得率最高。

The mechanism making the protein precipitate might be the main reason that caused polysaccharide losses while removing protein , with the protease is the best in taking off the protein and with the highest rate of Laminaria japonica Aresch polysaccharide .

麦汁澄清剂&卡拉胶能够加快麦汁中蛋白质沉淀，特别是十分有效地除去冷浊蛋白质，从而大大改善麦汁的浊度，提高啤酒的非生物稳定性。

Carrageenan , a kind of wheat juice clarifying agent , can fasten the precipitation of the protein especially cold-turbid protein in wheat juice . Therefore , it improves the turbidity of wheat juice greatly , and increases the non-biological stability of beer .

葡萄和葡萄酒中两种聚合色素：小分子色素（SPP）与蛋白质不产生沉淀，而大分子色素（LPP）则产生沉淀。

There are two kinds of polymeric pigments in grapes and in grape wines : small molecular polymeric pigments ( SPP ) ( not produce precipitate with protein ) and large molecular polymeric pigments ( LPP ) ( produce precipitate ) .

小麦蛋白质含量和沉淀值的遗传研究

Genetic Analysis of Protein Content and Sedimentation Value in Winter Wheat Grains

原核表达的蛋白质无论在沉淀中还是在上清中均有表达。

The protein of procaryotic expression exists both in the supernatant fluid and the sediment .

随着灌浆期日均温的升高以及日较差的增大，蛋白质含量与沉淀值的相关系数减小；

The correlation index between protein content and sedimentation volume decreased with mean temperature and daily temperature difference increasing during grain filling .

对黄酒酒液及沉淀物进行分析，确定高分子蛋白质在蛋白质沉淀中所起的作用。

High molecular weight ( HMW ) protein was a main factor in protein haze formation after analyzing rice wine and its sediment .

蛋白质含量、沉淀值以及面团形成时间与面包体积、面包总分有显著的正相关。

There are significant positive correlations between protein content and loaf volume and breed score , same for the sedimentation , dough development time .