油红

通过形态学观察、MTT比色、油红O染色提取法，比较各组细胞形态及增殖与分化的效果。

The proliferation and differentiation of preadipocyte were determined by MTT spectrophotometry and Oil Red O staining respectively .

CLA组大鼠肝脏切片油红O染色后肝细胞内的红染脂滴随CLA剂量的增加逐渐减少。

Fat droplets in liver tissue stained with Oil Red O were decreased as the doses of CLA increased .

当油酸作用肝细胞24h、48h、72h后采用油红O染色观察细胞内的脂滴的积聚情况。

The accumulation of lipid droplets in hepatocytes were observed by light microscopy after oil red staining .

利用细胞计数，细胞形态观察和油红O染色OD值测定等方法研究了CLA对大鼠前体脂肪细胞增殖与分化的影响。CLA可减少脂肪细胞数目。

By counting cell , determining OD after oil red 0 staining to investigate the effect of CLA on proliferation and differentiation of rat preadipocyte .

加入成脂诱导剂，油红O染色观察MSCs内脂滴形成；RT-PCR方法检测MSCs脂蛋白脂酶（lipoproteinlipase，LPL）mRNA的表达。

After adipogenic induction ( ADI ), lipid droplet in MSCs were detected by oil red O staining and the mRNA level of lipoprotein lipase ( LPL ) was measured by RT-PCR .

ADSCs成脂诱导后倒置显微镜下可看到大小不等脂滴，油红O染色阳性。

After adipogenic induction , fat droplets of verified size could be observed under phase-contrast microscope , with positive oil red O staining .

通过细胞形态观察、油红O染色、香草醛测总脂法和考马斯亮蓝测总蛋白等方法分析不同浓度AA和DHA对大鼠前体脂肪细胞分化的影响。

By observed cells morphologic changes , oil red o staining lipids assay , vanilline total fat and total protein assay method to detect regulation of AA and DHA on adipocytes differentiation .

用VOnKossa和碱性磷酸酶染色鉴定成骨细胞分化，而软骨细胞分化和脂肪细胞分化分别用alcianblue染色和油红O染色显示。

Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining , while chondrogenic and adipogenic differentiation were assessed by Alcian blue staining and Oil Red O staining respectively .

油红O染色、免疫细胞化学染色及RT-PCR证实ADSCs经诱导可分化为脂肪细胞及神经元样细胞。

ADSCs were stably proliferated and transferred in vitro till P8 . ADSCs differentiated into adipose cells and neuron like cells certified by Oil red O staining , immunocytochemical stain and RT-PCR .

油红O染色见泡沫细胞内有大量红色颗粒状脂滴，ox-LDL（50μg/ml）诱导组油红O染色阳性细胞数量明显高于未诱导组p0.05。

It can be seen a large number of red granular lipid droplets in foam cells by oil red O staining , Number of positive cells in induction group was significantly higher than non-induced group p0.05 .

结果油红O染色显示肿瘤细胞内有LCM的聚集，且多次注射LCM组其LCM浓度明显较单次注射组高。

Results Oil red O stain showed that LCM aggregated in tumor cells and the LCM density of multiple injection group was much higher than that of single injection .

结果膜状脂肪坏死的基本病理特点是：以一般脂肪坏死为背景，有囊型和非囊型两种特征性改变，膜状物油红O、PSA染色阳性。

Results Three kinds of histopathological changes were observed in 24 cases of MFN : a background of fat necrosis , cystic and non cystic configuration , and cystic membranes stained by oil red O and PAS .

结果：OXLDL刺激下系膜细胞TC含量及油红O染色阳性细胞比例均较无脂蛋白刺激的照系膜细胞增高，电镜下见泡沫细胞。

Result : Foam cells were observed in mesangial cells stimulated by OX LDL , and both the TC content and the proportion of lipid droplet containing cell were higher in these cells than in mesangial cells cultured in lipid free conditions .

方法1.体外分离、纯化、培养和鉴定大鼠睾丸支持细胞：取18~21日龄雄性SD大鼠的睾丸组织，体外原代培养睾丸支持细胞，苔盼蓝鉴定细胞活力，油红O鉴定细胞纯度。

Isolation , purification , culture and identification of rat Sertoli cells in vitro : Take 18 to 21 day-old male SD rat testis , culture primary Sertoli cells in vitro , identify cell viability by trypan blue , and identify cells purity by oil red O.2 .

用0.1mg/ml油红O染色2小时，倒置显微镜下观察结果，拍摄图片。

After stained with 0.1 mg / ml Oil Red O solution for 2 hours , observe the results with inverted microscope and take photos .

结果与ox-LDL诱导组相比，Nef保护组巨噬细胞的油红O染色阳性细胞数和细胞内脂质含量均显著减少；

Results Compared with ox-LDL group , oil red O-positive cells of Nef protective group were greatly reduced . The cellular contents of total cholesterol and cholesterol ester of Nef protective group were also obviously decreased .

通过检测大叶紫薇中总三萜对3T3-L1葡萄糖的消耗，油红O染色并通过比色定量分析脂肪含量。

The glucose consumption in3T3-L1 was investigated.The3T3-L1 cell was stained by oil red O for analyzing the fat content by spectrophotograpy quantitatively .

油红染色显示，皮脂腺细胞含有少量脂质小滴，CK4.62、EMA免疫组织化学染色均为阳性；

The results of oil red staining indicated that a small quantity of lipid droplets in sebocytes , and immunohistochemistry staining of CK4.62 , EMA were positive in subculture sebocytes .

以VLDL温育细胞，检测细胞内脂质含量，油红O染色法观察胞内脂质堆积及泡沫细胞形成情况。

After incubated with VLDL for different time , the contents of triglyceride and total cholesterol in cells were measured , and the formation of foam cells and accumulation of lipid in cells were observed by oil-red O staining .

共培养之大鼠骨髓间充质干细胞分别经诱导分化为成骨细胞和脂肪细胞形态（分别经碱性磷酸酶、VonCossa染色和油红染色证实）。

After induction , the Co-cultured cells appeared morphological changes of osteoblast and adipocyte , and were confirmed to be osteoblast by alkaline phosphatase , Von Cossa staining , and be adipocyte by oil red staining .

结果显示，72h后，反义寡核苷酸处理组细胞内胆固醇酯显著下降到（199±19）mg/g，油红O染色显示，该组细胞质内红色脂滴明显减少。

The results shown , the antisense oligonucleotides treated cells contained significantly lower cholesteryl ester ( 19.9 ± 1.9 ) mg / g for 72 h. It was also shown by Oil Red O staining that the experimental group cells had less lipid droplets .

取第3代ADSCs进行成脂、成骨诱导分化，两周后油红O成脂染色及三周后茜素红成骨染色鉴定。

The third passage ADSCs were inducted and differentiated into adipogenic cell line and osteogenic cell line . Oil O red staining and alizarin staining were performed after two and three weeks of induction to identify the differentiation of ADSCs . 3 .

方法：培养3T3-L1脂肪细胞，促分化成熟后，光镜下观察细胞形态的变化，油红O染色鉴定脂肪细胞。

Methods : 3T3-L1 adipocytes were cultured and differentiated into mature adipocytes in vitro . Their morphology change was observed through microscope and oil red O stain was taken to identify the adipocytes .

油红O检测细胞内脂质；

The lipid within endothelial cells was detected with oil red O.

曲格列酮组随着药物浓度增高，油红O染色明显增强。

Oil red O became more obvious when concentration was increased .

方法油红O染色法测定细胞分化速度；

Methods Cell differentiation was determined by Oil Red O staining ;

油红O染色检测细胞内脂滴。

Lipid droplets in the cells were stained with Oil Red O.

油红O染色发现试验组乳腺上皮细胞中脂滴含量的增加。

Lipid droplets increased in mammary epithelial cells of experiment group .

油红O染色观察肝组织脂肪浸润；

Liver fatty changes were evaluated with oil red O staining .

成脂定向诱导分化后油红O染色定性。

Adipogenic differentiation of ASCs was assessed by Oil Red O staining .