多克隆位点
duō kè lónɡ wèi diǎn
- 名multiple cloning sites;multicloning site;polycloning sites
多克隆位点-
采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。
Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers , a novel megaprimer method was performed in one tube with two steps and34 cycles .
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随机选取50个克隆，用位于pRAC质粒多克隆位点两侧的测序引物进行菌落PCR扩增，检测文库插入片段的分布情况。
50 clones were selected randomly and the colony PCR amplification was used to detect the distribution of library inserts with pRAC sequencing primers .
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方法：通过双酶切把PScDNA全长序列接到pBlueskriptⅡKS（+）的多克隆位点上；
Methods : Link the PS cDNA full-length sequence to the multiple cloning site of pBlueskript ⅱ KS ( + ) by double restriction enzyme digest .