寡聚核苷酸

方法本文根据G蛋白多肽碱基序列的需要，设计并合成了带有突变核苷酸序列的寡聚核苷酸引物，以G基因RT-PCR产物为模板，采用高保真DNA聚合酶，PCR突变扩增，得到G蛋白多肽编码序列。

Methods : Based on the base sequence of the G-protein polypeptide , the oligonucleotide primers were designed and synthesized which contains the mutational nucleotide sequence .

反义技术是根据碱基互补原理，利用与靶DNA或靶RNA互补的短链寡聚核苷酸片段抑制基因表达的方法。

Antisense technique , developed on the principle of base complementation , is a method using short strand oligonucleotide fragments complementary to target DNAs or target RANs for inhibition of gene expression .

结果：PAGE电泳和DNA测序分析证实定向插入的寡聚核苷酸串与预先设计的插入序列一致。

Results : Both PAGE electropheresis and DNA sequence analysis confirmed the inserted oligonucleotides .

与传统的作用于蛋白质的药物相比，反义寡聚核苷酸作用于RNA。

As compared to conventional drugs that work on proteins , antisense oligonucleotides work on RNA .

制备脂质体包裹的~（99m）锝标记c-MycmRNA反义寡聚核苷酸的研究

Preparation of Liposome-mediated ~ ( 99 ) m-technetium-labeled Antisense Oligonucleotides of c-myc mRNA

多聚酶链反应寡聚核苷酸探针反向杂交在HLAⅠ类抗原分型中的应用

Application of polymerase chain reaction sequence specific oligonucleotide probes reverse hybridization in HLA-A and HLA-B typing

硫代反义寡聚核苷酸对HSV的作用及对小鼠HSV角膜炎发病的影响

Effect of phosphorothioate antisense oligonucleotide on HSV and mice HSV keratitis

端粒酶是一种特殊的逆转录酶，它能在体内延伸染色体单链末端，在体外延伸寡聚核苷酸。一般认为端粒酶的蛋白催化亚基和RNA亚基组成了核心酶，它们对体外活性是必需的。

Telomerase is a specialized reverse transcriptase that elongates the single-stranded chromosome ends and oligonucleotides in vivo and in vitro .

HBV-DNA的生物素寡聚核苷酸探针快速检测

Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe

从40个寡聚核苷酸随机引物中筛选出17个扩增重复性好、条带清晰、特异性强的引物，对每个个体基因组DNA进行了扩增。

Of 40 random oligonucleotide primers for the amplification of amphioxus genomic DNA , 17 could produce reproducible , distinctive and characteristic fingerprints varying in length from 200 to 2 200 bp .

人工设计并合成了抗菌肽B基因的4个寡聚核苷酸片段，通过重叠区扩增法，扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。

Designed and synthesised 4 fragments of antibacterial peptide B gene , then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension ( gene SOEing ) .

我们用改进了的寡聚核苷酸诱导突变法，将两个单一限制性内切酶位点引入人胰岛素前体B链与C链连接处附近，及C链与A铁连接处附近。

Two restriction endonuclease cleavage sites near the junction of B chain and C chain and the junction of C chain and A chain on the human proinsulin gene were created by improved oligonucleotide-directed mutagenesis .

以寡聚核苷酸为探针对癌基因Ha-ras点突变的测定

The Detection of Point Mutation Of Oncogene HA-RAS With Synthetic Oligonucleotide Probes

TbL表现出对单链寡聚核苷酸和小牛胸腺DNA中的腺嘌呤以及鸟嘌呤碱基的选择性荧光增强。

TbL exhibits a remarkable selectivity for adenine and guanine nucleobases in single-stranded oligonucleotides and calf thymus DNA , accompanied with a significant enhancement in luminescence .

反义寡聚核苷酸对CVB3蛋白基因表达的抑制作用及量效关系的研究

Antisense oligonucleotide inhibition of coxsackievirus B3 gene expression in HeLa cells and dose-response experiments

目的比较多聚酶链反应寡聚核苷酸探针（PCR-SSOP）技术与血清学方法对HLAⅠ类抗原A、B分型的准确性及分型精度。

Objective To compare the accuracy and sensitivity of polymerase chain reaction se - quence specific oligonucleotide probes ( PCR-SSOP ) reverse hybridisation vs serological method for HLA - A and - B typing .

核酸切割试剂与寡聚核苷酸（ODN）偶联制得的人工核酸酶能在特定位点断裂DNA或RNA，为人工核酸酶的分子设计提供了一种新方法。

The artificial enzymes , which are prepared by cleavage reagents of nucleic acid conjugated to a synthetic oligodeoxyribonucleotide , can selectively cleave DNA or RNA . It provides a new method of the molecular design of artificial nuclease .

已显示反义分子对基因有调控作用，以反义技术组建基因工程反义RNA或人工合成反义寡聚核苷酸，特异性地阻断基因的转录、翻译，可作为高度专一性的基因调控工具。

Antisense molecules can regulate and modify gene expression . By using antisense technology , antisense RNA or artificial antisense oligonucleotides can be made to inhibit or block the transcription and translation of genes . These molecules are highly specific in gene regulation .

对金基体上自组装寡聚核苷酸探针杂交前后进行电化学非现场及现场表面增强拉曼光谱（SERS）研究。

A detailed study is conducted for the electrochemical ex situ and in situ surface enhanced Raman scattering ( SERS ) studies of oligonucleotide probe self-assembled monolayer on gold substrate before and after hybridization .

LPA法利用“酶切-连接-PCR”之组合，首先选定一组限制性内切酶，各自单独地酶切细菌总DNA样品，然后酶切产物分别与相应的寡聚核苷酸片段接头连接。

Bacterial genomic DNA samples were first digested by a group of selected restriction enzymes , separately . The resulting digests were then ligated to one of the three types of specially designed adapters corresponding to the restriction enzymes used for digestion .

我们采用地高辛（digoxigenin）标记的寡聚核苷酸探针和原位杂交技术研究了新发现的阿片受体样受体ORL1基因在正常大鼠脑内的表达和分布。

In situ hybridization with a digoxigenin-labeled oligonucleotide probe was used to investigate the expression of opioid-receptor-like receptor ( ORL1 ) gene in rat brain .

Pg与口腔感染性疾病关系密切.现根据Pg特异的菌毛亚单位蛋白结构基因设计寡聚核苷酸引物，采用PCR技术扩增了其中长542bp的片段并对其进行了特异性鉴定。

Ated with some oral infectious diseases . In the present study , the oligodeoxynucleotide primers were designed to amplify specifically a segment of the subunit protein fimbrial gene of Pg. The specific product of 542-bp was amplified by polymerase chain reaction ( PCR ) .

我们发现这些带有保护基的寡聚核苷酸在DEAE-葡聚糖A-25（Cl~-型）柱上，用含有3M尿素和40%乙醇的氯化锂溶液洗脱，可以克服这一困难。

This difficulty can be overcome by separation on a DEAE-sephadex A-25 ( Gl-form ) column instead of a DEAE-cellulose column , followed by elution with lithium chloride solution containing 3M urea and 40 % ethanol .

为研究犬瘟热病毒（CDV）融合蛋白各段功能，根据CDVOnderstepoort弱毒株的融合蛋白基因序列，设计合成了10条寡聚核苷酸引物。

Ten oligonucleotide primers were designed and synthesized according to the nucleotide sequence of the fusion protein gene of the attenuated Onderstepoort strain of canine distemper virus ( CDV ) to provide the basis for study the function of CDV fusion protein .

硫代反义寡聚核苷酸在线性聚丙烯酰胺-毛细管电泳中的分离行为

Separation Investigation of Antisense Phosphorothioate Oligonucleotides Using Capillary Electrophoresis with Linear Polyacrylamide

寡聚核苷酸标记纳米金可视化检测三聚氰胺

Colorimetric detection of melamine using gold nanoparticles capped with oligonucleotides

磁性纳米人工切断试剂定位水解单链寡聚核苷酸的研究

Development of Magnetic Nanoparticle-Based Artificial Cleavage Reagent for Sequence-Specific Hydrolysis of Single-Stranded DNA

用阴离子交换色谱法制备寡聚核苷酸

Separation of oligodeoxynucleotide by high performance anion exchange chromatography

反义寡聚核苷酸：生理学研究中的新工具

Antisense oligonucleotides : new tools for physiological study

寡聚核苷酸中鸟苷对四甲基罗丹明的荧光猝灭

Fluorescence quenching of TMR by guanosine in oligonucleotides