基因突变率

绵山羊不同性别的基因突变率及其系统进化的研究

Studies on Gene Mutation Rate of Different Sex and Phylogeny of Sheep and Goats

最后讨论了基因突变率与突变概率间的相互关系及本模型在多性状分析中的应用问题。

Lastly , the relationship between gene mutation rate and mutation probability and the application of the model in the analysis of multiple characters are discussed .

同时应用DNA序列分析确定gsp癌基因突变率。

Mutation rate of gsp oncogene was identified by DNA sequential analysis .

组织粪便APC基因突变率相比差异无统计学意义（χ2检验P＞0.05）。

There was no difference between the positive rate of the tumor tissues and stools by chi-square test ( P ﹥ 0.05 ) .

结直肠癌k-ras基因突变率及相关因素的研究

The Mutation Rate of k-ras and Related Factors in Colorectal Cancer

结果全组CKIT蛋白的阳性率为97.6%（80/82），CKIT基因突变率为41.5%（34/82）。

Results The positive c kit protein expression and c kit gene mutation rates were 97.6 % ( 80 / 82 ) and 41.5 % ( 34 / 82 ) .

[结论]宫颈癌组织中p53基因突变率较低，提示p53基因突变并非宫颈癌组织中p53蛋白异常表达的主要原因。

[ Conclusion ] Low frequency of p53 gene mutation in cervical cancer tissues suggests that p53 protein abnormal accumulation may not result from p53 gene mutation .

rrs基因突变率依次分别为0、12.5%、7.2%。

The mutation rate of gene rrs is 0,12.5 % , 7.2 % respectively .

在25例肺腺癌中，Kras基因突变率为48.0%，其它类型肺癌中突变率仅为8.8%，而14例良性组织中未检测出Kras基因突变。

The mutation rate of K ras gene was 48.0 % in adenocarcinomas . No K ras gene mutations was detected in the specimens of benign respiratory diseases .

用多核细胞法检测细胞次黄嘌呤鸟嘌呤核糖转移酶（HPRT）基因突变率；

Multinuclear cell assay was used to detect the frequency of the HPRT gene mutation ;

方法采用RT-PCR技术和聚合酶链式反应-单链构像多态性分析（PCR-SSCP）方法，对46例结直肠癌的新鲜癌组织进行P53、K-ras基因突变率和VEGF表达率的检测。

Method RT PCR and PCR SSCP were used to detect P53 , K ras gene and VEGF in primary foci of 46 cases with colorectal cancer .

方法应用多核细胞法检测外周血淋巴细胞HPRT基因突变率。

Methods HPRT gene mutation frequency ( Mf ) was examined by the technique of multinuclear cell assay .

[结果]肺癌患者Kras基因突变率为355%（22/62），肺良性疾病中未发现有Kras基因突变者。

K ras mutation rate in patients with lung cancer was 35 5 % ( 22 / 62 ), no mutation was found in patients with benign pulmonary diseases ( 0 / 20 ) .

3B、3C和3D基因突变率低，序列比较保守，这与各自的功能有一定的关系。

The 3B , 3C and 3D gene mutation rates are low , the sequence is more conservative , this have the certain relationship with the respective function . relation .

结果：PCR-SSCP检测舌白斑和舌癌p53基因突变率分别为20.0%（2/10）和59.4%（19/32）。

Results : PCR-SSCP results showed that the mutation rate was 59.4 % ( 19 / 32 ) in TSCC and 20.0 % ( 2 / 10 ) in tongue leukoplakia .

为了研究丙烯酰胺的遗传毒理作用，采用单细胞克隆培养，双向筛选计数，多重PCR扩增与电泳分析，研究了诱导HL60和NB4两种细胞hprt基因突变率及分子突变谱。

The genotoxicity of acrylamide was investigated by methods of single cell clone culturing , two way screening count , multiplex PCR amplification and electrophoresis technique .

结果肺癌组织中p53基因突变率为37.3%（22/59），痰液脱落细胞为33.9%（20/59），两组间比较无显著性差异（P>0.05）。

Results The mutation rate of p53 was 37.3 % ( 22 / 59 ) in cancer tissues , and 33.9 % ( 20 / 59 ) in sputum cells . No significant difference was observed between the two groups ( P > 0.05 ) .

结果：子宫内膜癌组织中PTEN基因突变率和蛋白缺失率分别为30.6%和61.1%，高于正常子宫内膜组织（0）（P<0.05）。

Results : The rates of mutation and protein expression deletion of PTEN in endometrial carcinoma tissues were 30.6 % and 61.1 % respectively , which were higher than that of normal endometrial tissues ( P < 0.05 ) .

端粒酶活性表达组p53基因突变率44.74%（17/38），未表达端粒酶活性组p53基因突变率12.50%（1/8），组间比较有统计学意义。

The rate of p53 gene mutations in group of expression of telomerase activit was 44.74 % ( 17 / 38 ), and was ( 12.50 % )( 1 / 8 ) in without expression of telomerase activit . There was significant difference between the two groups .

PTEN蛋白表达完全缺失在G2、G3级肿瘤高于G1级（P<0.05），PTEN基因突变率和蛋白缺失率均与肌层浸润、淋巴结转移及手术-病理分期无明显关系（P>0.05）。

The PTEN protein expression deletion in pathological G2 , G3 was higher than that of G1 ( P < 0.05 ), but both of the gene mutation and protein expression deletion of PTEN were no significant difference in different clinical stage .

联合检测肺癌患者痰液脱落细胞p53和Kras基因突变率为54.2%（32/59），显著高于痰脱落细胞中33.9%的p53突变和20.3%的Kras突变率。

The mutation rate ( 54.2 % ) of combination detection of p53 and Kras in sputum cells was significantly higher than that of p53 ( 33.9 % ) or Kras ( 20.3 % ) alone ( P < 0.05 ) .

肺癌组织和患者痰液脱落细胞中Kras基因突变率分别为23.7%（14/59）和20.3%（12/59）。

The mutation rate of Kras gene was 23.7 % ( 14 / 59 ) and 20.3 % ( 12 / 59 ) in cancer tissues and sputum cells respectively .

扣除NNK效应后，α粒子和NNK联合作用诱发BEP2D细胞DNA损伤、HPRT基因突变率、以及细胞微核率也明显高于α粒子单独照射组。

Subtracted the NNK effect , DNA damage , HPRT gene mutation frequency and cell micronucleus frequency in the group irradiated by alpha particles in combination with NNK administration were significantly higher than those of alpha particles irradiation alone .

结果与相同剂量NNK或α粒子单独作用比较，α粒子和NNK联合作用诱发BEP2D细胞DNA损伤、HPRT基因突变率、以及细胞微核率均显著增高；

Results In the group exposed to both alpha particles irradiation and NNK , DNA damage , HPRT gene mutation frequency , and cell micronucleus frequency were significantly higher than those in the same dose groups irradiated with alpha particles or NNK administration alone .

在胰腺癌中，K-ras基因突变率平均在80%左右，其中大于98%的突变形式是第12位密码子的点突变，以GAT（46%）形式最为多见。

About 80 % of pancreatic ductal cancers have an activating point mutation in the K-ras gene , above 98 % is the codon 12 mutation and the most common amino acid substitution is aspartic acid ( GAT ) for glycine ( 46 % ) .

1例胰腺癌可见异常泳动带，基因突变率为2.2%（1／46）。

Intragenic mutation was found in one of the 46 cases .

自然选择倾向于保持一个使可进化性最大化的基因突变率。

Natural selection tends to maintain a mutation rate for maximal evolvability .

20例良性肺疾病支气管肺组织p53基因突变检出率为5%。故活检组织p53基因突变检出肺癌的敏感性为60%，特异性为95%；

In patients with benign pulmonary diseases , p53 point mutation in bronchial biopsy sample was detected in only one case ( 5 % ) .

方法应用PCR-SSCP技术检测62例胃癌手术患者门静脉血中p53和K-ras基因的突变率。

Methods p53 and K-ras gene mutation was detected with PCR-SSCP technology in 62 cases of gastric carcinoma .

健康成人和放射工作者体细胞HPRT基因位点突变率的检测和比较

Detection and comparison of mutant frequency of somatic HPRT locus between healthy adults and radiation workers