单细胞

Air（初三适用）甘薯原料气升式生物反应器发酵生产单细胞蛋白

Single Cell Protein Production From Dried Sweet Potato Using Air Lift Bioreactors

单细胞凝胶电泳检测DNA辐射损伤的剂量-效应关系研究

Study on dose-effect relationship of radiation-induced DNA damage using single cell gel electrophoresis

单细胞凝胶电泳能够较灵敏地反映人群因有毒化学物质造成的DNA损伤，在分子流行病学研究中具有重要的应用价值。

SCGE could be used in molecular epidemiological study .

采用单细胞凝胶电泳法检测DNA自发及诱导氧化损伤；

DNA damage were analyzed by SCGE ;

单细胞电泳观察重离子辐照人肝癌细胞所致DNA损伤

Observation of DNA damage of human hepatoma cells irradiated with heavy ions using comet assay

应用荧光PCR技术检测单细胞β地中海贫血基因

Fluorescent PCR for Detection of β Thalassemia Genes in a Single Cell

改良单细胞凝胶电泳技术在DNA损伤与修复检测中的应用

Application of the technology of single-cell gel electrophoresis improvement to the detection of DNA damage and repair

单细胞PCR对种植前胚胎的性别鉴定

Sex determination of preimplantation embryos by Nest PCR

单细胞凝胶电泳（彗星实验）检测细胞DNA的损伤。

DNA damage of germ cells was assessed by single cell gel electrophoresis ( SCGE or Comet assay ) .

单细胞逆转录PCR研究方法及其应用

Single cell RT-PCR technique and its application

单细胞分离及单细胞PCR技术在淋巴结生发中心研究中的应用

Preliminary Study of Single Cell Isolation and Single Cell PCR in Lymph Node Germinal Centers

用巢式PCR、引物延伸预扩增法扩增单细胞，扩增率分别为90%和85%。

Amplification rate of nest-PCR and PEP was 90 % and 85 % , respectively .

应用单细胞凝胶电泳比较研究砷对人类细胞DNA的损伤

Application of single cell gel electrophoresis ( SCGE ) assay : comparative study of DNA damage induced by arsenic in human cells

于胞蚴的胚球，尾蚴的吸盘、生殖原基、单细胞腺体主要含DNA阳性物质；

Germ balls in daughter sporocyst , suckers of the cercaria , germinal primordia and unicellular gland of the cercaria contain DNA positive substances .

应用碱性单细胞凝胶电泳技术（SCGE）检测镉致鸡脾淋巴细胞DNA的损伤效应

Effects of Cadmium on DNA Damage in Chicken Splenic Lymphocytes with Alkaline SCGE

采用单细胞凝胶电泳检测小鼠睾丸细胞DNA受损程度，显示DNA迁移细胞数和彗尾长度上升。

DMA damage were detected by single-cell gel electrophoresis assay . After exposure the relative tail length of the comets and cells with DNA migration were increased .

方法采用聚苯乙烯凹槽单层铺胶后进行碱性单细胞凝胶电泳，观察γ射线外照射引起的人外周血淋巴细胞DNA损伤效应。

Methods The radiation-induced DNA damage in human peripheral blood lymphocytes was e-valuated using alkaline single cell gel electrophoresis by filled the polystyrene ponds with mono-layer gel embedded lymphocytes .

方法采用中性单细胞凝胶电泳检测低剂量辐射对荷瘤鼠放疗后外周血淋巴细胞DNA双链断裂的情况。

Methods Neutral single-cell gel electrophoresis was used to detect the effects of low dose radiation on DNA double strand breaks of peripheral blood lymphocytes in tumor-bearing mice after radiotherapy .

方法对口服CⅡ致耐大鼠进行特异和非特异性淋巴细胞增殖实验，并采用酶联免疫斑点法（ELISPOT），对CⅡ特异性T细胞在单细胞水平进行细胞内细胞因子检测。

Methods Specific and non specific lymphocyte proliferation test and enzyme linked immunospot ( ELISPOT ) assay were used in the present study .

目的：探讨对单细胞凝胶电泳法（SCGE）检测细胞核DNA损伤的影响因素。

Objective : To study influential factors of single cell gel electrophoresis ( SCGE ) assaying nuclear DNA damage .

方法DNA损伤采用单细胞凝胶电泳（彗星实验）法，代谢酶活性采用分光光度法，肝功能测定使用自动生化分析仪。

Methods DNA damages were detected by single cell gel electrophoresis ( comet assay ) . The activities of metabolizing enzymes were measured by spectrophotography , and hepatic function was determined by biochemical analyser .

原代培养细胞在无血清培养基中生长2～3d后，大部分细胞死亡，只有少数以单细胞形式悬浮生长。

Most of the primary culture cells were dead after cultured in serum-free medium for two or three days , most of the cells died , only a few of single cells survived in suspension .

单细胞凝胶电泳（SCGE）的结果显示，牡蛎鳃细胞DNA的损伤程度与TBT浓度存在明显的剂量-效应关系。

The results of single cell gelelectrophoresis ( SCGE ) showed that there was a significant dose-effect between TBT concentrations and DNA damage of gill cells .

单细胞水平检测vonHippel-Lindau病基因突变的实验研究

The detection of mutations in VHL gene from a single cell in a patient with von Hippel-Lindau disease

回顾了国内外有关AFM在单细胞表面形态与结构、活细胞的力学响应和生理过程的监控、细胞间粘附力的测量和肿瘤的科学诊断等研究中的应用情况；

Reviewed the application states of the AFM in that of the surface configure and structure , mechanic response , physiological inspecting , tumor diagnosis and adherence force between cells ;

用单细胞培养法，从人胃癌细胞系BGC-823中分离出10个克隆。

Ten cell strains were obtained from the human gastric cancer cell line BGC-823 by using the method of single cell cloning .

PEP、DOP-PCR单细胞多基因位点检测研究

Genotyping analysis of multiple loci in single cell by primer extension preamplification and degenerate oligonucleotide primed - PCR

目的：传统的Ames实验、体外哺乳动物细胞染色体畸变实验、单细胞凝胶电泳实验已广泛用于化学品毒性的监测，但用于综合评价化学品的遗传学毒性还不够完善。

Objective : Traditional Ames test , in vitro mammalian chromosome aberration test , single cell gel electrophoresis assay have been used widely in monitoring chemical toxicity .

方法①用过氧化氢（H2O2）诱导DNA损伤，采用单细胞凝胶电泳方法测定DNA氧化损伤程度，同时测定大鼠血清丙二醛（MDA）和脂褐质（LPO）的含量；

Methods DNA damage was induced by H2O2 , then the extent of damage was determined by single cell gel electrophoresis technique ( SCGE ) . The contest of MDA and LPO of rat serum were measured by biochemical method .

将HT显微镜与微流控分析技术联用，发展了一种单细胞进样与在线成像分析新技术，并将其应用于花粉单细胞成像分析方面。

By combining the HT microscopy with the microfluidic analysis technique , a novel method for single-cell sampling and on-line imaging is developed . This method is applied to single-cell imaging analysis of pollen cells .