低拷贝

低拷贝模板DNA分析技术研究进展

Recent development in Analysis Method of Low Copy Number DNA

一种有效纯化低拷贝质粒DNA的改良硅藻土法

An Improved Protocol for Low Copy Number Plasmid DNA Purification with Diatomaceous Earth

这为低拷贝或单一拷贝等小片段DNA物理定位的可靠性以及它们共杂交的可行性提供了令人信服的证据。

It demonstrates that the in situ hybridization location of low or single copy DNA is reliable completely .

近年来，低拷贝模板类生物物证受到广泛关注并在法医DNA分析中占有了越来越重要的地位。

In recent years , Low Copy Number biological evidence plays an increasingly important position in forensic DNA analysis .

PCR扩增循环数与低拷贝模板DNA的STR分型

A study on the STR genotyping of low copy number by increasing PCR amplification cycles

PCR扩增3因素对低拷贝模板STR分型的影响研究

A study on the influence of three factors in PCR to LCN-STR genotyping

纳米金增强复杂体系中PCR扩增低拷贝基因的反应特异性初步研究

Preliminary Research on Gold Nanoparticles Enhancing the Specificity of PCR Amplification of Low Copy Gene from the Complicated System

目的探讨PCR扩增循环数对低拷贝模板DNA的STR分型的影响。

Objective To investigate the influence of increasing PCR amplification cycles on low copy number DNA genotyping .

Southern杂交结果表明它们在陆地棉以及海岛棉基因组中属低拷贝基因，并且具有多个同源基因。

Southern hybridization indicated all of them are low copy genes and have a few of homologous genes in G. hirsutum and G. barbadense .

研究表明，多倍体小麦基因组中存在一类低拷贝、染色体专化的DNA序列，其在多倍体形成时常表现出不稳定性。

Recent work revealed that , in the genomes of polyploid wheat , there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation .

目标基因属于解旋酶（Helicase）基因家族。通过与香蕉基因组进行Southern杂交分析表明：证实此解旋酶基因来自香蕉，在香蕉基因组中只有一个拷贝，为低拷贝基因。

The result of Southern blotting indicated that it came from banana and only low copy .

目的探索应用原位逆转录PCR（RT-PCR）检测低拷贝的干细胞因子mRNA在白血病细胞表达的可行性。

Objective To evaluate the feasibility of detecting low copy stem cell factor ( SCF ) mRNA in leukemic cells by in situ reverse transcriptase-PCR ( RT-PCR ) .

通过Southernblot分析目的基因在月季基因组中存在的拷贝数，发现eIF-5A在月季基因组中以低拷贝形式存在。

By Southern blot , we analyzed the copy number of this gene in Chinese Rose genome , finding low-copy in Chinese Rose genome . 2 .

pUC衍生质粒的一个低拷贝数突变型

A Low Copy Number Mutant of pUC-Derived Plasmid

可见，该方法显著提高了小麦RAPD分析的多态性水平。而且，由于PAGE能够稳定检出低拷贝的扩增片段，也改善了RAPD分析的重复性。

The results suggested that using denaturing PAGE-silver staining could remarkably increase the level of DNA polymorphism detected in wheat , and could improve the repeatability of RAPD analysis because of effective detection of low copy DNA fragments .

结论干扰素α-1b是目前治疗慢性乙型病毒性肝炎较为有效且安全的可选择的药物，尤其对女性、高ALT水平、HBV-DNA低拷贝数（≤106copies/ml）的慢性乙肝患者更为有效。

Conclusion α - 1b interferon is one of the effective and safety medicines for treating CHB especially to females or those with higher ALT level or HBV-DNA ≤ 106 copies / ml.

经RT-PCR分析，低拷贝数的转基因后代显阳性反应，多拷贝数的显阴性反应，说明T-DNA多拷贝数影响外源基因的表达。

Through RT-PCR analysis , low copy number transgenic plants showed positive reaction and multi-copy number showed negative reaction . It was suggested that the expression of foreign gene was influenced by the T-DNA copy number .

目的建立基于多重置换扩增（MDA）技术的全基因组扩增（WGA）方法，实现对低拷贝数（LCN）DNA样品进行分析。

Objective To establish a method for whole genome amplification ( WGA ) based on ( multiple displacement amplification MDA ), and achieve DNA analysis of the human genome from low copy number ( LCN ) .

结果对低拷贝模板DNA，单纯增加Taq酶量或反应体系，扩增效率改善不明显；增加循环数，显著提高检验灵敏度；

Results For LCN template DNA , if only increased the amount of Taq enzyme or the reaction volume , the results of STR amplification were scarcely improved , but if increased PCR cycles , the detection sensitivity were significantly improved .

本试验尝试利用具有极高灵敏度的原位PCR方法，从组织中检测低拷贝甚至单拷贝的目标序列，以鉴别隐性感染或混合感染，为IBDV的诊断和分子流行病学的分析提供了一种新的手段。

We tried to detect low , even single target sequence for identification of silent or mixed infection by in situ PCR which the sensitivity is very high . It is practicable to apply in situ PCR on clinical diagnosis and molecular epidemiological investigation of IBDV .

对比分析提示，肺结核病人MIF基因启动子区CATT低拷贝数（低于或等于6个）出现的频率明显高于正常人（85.6%比66.6%），可能同结核病的致病相关。

The rate of low copy number of CATT repeat ( less or equal to 6 ) was significantly higher in lung tuberculosis patients than in normal people ( 85.6 % vs 66.6 % ), suggesting that MIF might play a reciprocity in the pathogenesis of tuberculosis .

而单、低拷贝序列却未检测到杂交信号。

But no signal was detected with the low / single copy probe .

本课题组构建的转基因番茄植株属低拷贝的转基因植物，具有较好的稳定性。

The transgenic tomato plants which has high stability are low-copy transgenic plants . 3 .

剩余的4个克隆据分析有可能是单拷贝或低拷贝序列，我们尝试对它们进行染色体定位。

Hybridization with the 4 clones which were likely to be low or single copy sequences was then attempted .

碱法制备的低拷贝质粒因含有大量杂质而无法获得有效的测序结果。

Sequencing of low copy number plasmid DNA failed quite often due to high amount of impurities in sample .

单、低拷贝序列占39.6%，中、高拷贝序列占50.4%，质粒空载率平均约为10%。

It consisted of 39.6 % single / low-copy-sequence clones , 50.4 % medium / high-copy-sequence clones and 10 % empty clones .

由此构建的原生质体转化体系具有低拷贝、转化率较高，遗传稳定强的特点，不失为一种丝孢类生防真菌遗传操作的有效方法。

The transformation system featured with lower copies , higher efficiency and genetic stable has proven useful for genetic manipulation of the filamentous fungal biocontrol agent .

同时这类载体在宿主细胞中以低拷贝数存在，并以依赖细胞周期的形式复制到子代细胞中。

Moreover , the episomal vectors exist in host cells at a low copy number and are duplicated to the progeny cells by depending on the cell cycle .

本文还对11个低拷贝的转座子和9个核基因的甲基化模式进行了研究。

We also found that DNA methylation patterns of 11 selected low-copy mobile elements and 9 nuclear genes were altered in some of the laser-irradiated rice M0 plants .

在这类植物谱系中，低拷贝核基因具有特殊的进化特点和丰富的植物系统发生信息，在转录水平存在基因沉默、基因激活和不均等表达等特点。

The low-copy nuclear genes with special evolution characteristics and abundant phylogenetic information in polyploidy plant lineage show gene silencing , gene activation , and unequal expression in transcript level .