顺式作用元件

目的：构建受AFP顺式作用元件调控的超抗原表达载体，将SEA（D227A）特异性的表达于AFP阳性肝癌细胞膜表面。

AIM : To construct the superantigen SEA ( D227A ) expression vector modulated by cis acting element of AFP and express it specifically on AFP + hepatocellular carcinoma ( HCC ) cells .

雌激素调节人血管紧张肽原基因表达的顺式作用元件分析

Analysis of a cis Acting Element Involved in Regulation by Estrogen of Human Angiotensinogen Gene Expression

转录因子是一种DNA结合蛋白，通过与真核基因启动子区域中的顺式作用元件发生特异性相互作用来调控基因的转录。

Transcription factors are DNA-binding proteins that interact with cis DNA elements in regulating gene expression .

GC盒不是人胰岛素基因启动子中关键顺式作用元件

GC - box is not a key cis - acting element in human insulin gene promoter

W盒存在很多病原菌诱导的基因启动子中，是参与病原菌应答的一类主要顺式作用元件。

W-boxes are a major class of cis-acting elements responsible for the pathogen inducibility of many plant genes .

已有研究表明DNA顺式作用元件与反式作用因子之间的相互作用是染色质重塑和基因转录调节的基础。

Present studies indicate that the interactions between trans-acting factors and corresponding DNA cis-acting elements are the basis of chromatin remodeling and gene transcription regulation .

多个顺式作用元件调控t-PAcDNA乳腺定位表达

Multiple Cis acting Elements Regulating t PA cDNA Expression in Mammary Gland

目的鉴定人胰岛素基因启动子中的GC盒（-348￣-338）是否是启动子中的关键顺式作用元件。

Objective To identify whether GC-box ( - 348 to - 338 ) in human insulin gene promoter is a key cis-acting element .

GT元件是位于植物基因启动子区域中的串联重复DNA序列，是启动子中富含T和A的顺式作用元件。

GT elements are DNA sequences occurred in tandem repeats in the promoter region of plant genes , which are rich in nucleotides T and A in promoters .

转录因子是一种能够调节靶基因表达水平的DNA结合蛋白，它通过与靶基因启动子区域的的顺式作用元件结合，在mRNA转录水平上调控靶基因的时空表达强度。

Transcription factor ( TF ) is a kind of DNA-binding protein which can bind to cis-acting elements in gene promoter to ensure mRNA copies of target genes in one particular time and space spot .

DRE顺式作用元件dsDNA芯片制备

Manufacture of dsDNA Microarrays of DRE Cis-acting Element

肺癌细胞中调控ezrin基因基本转录活性的顺式作用元件及转录因子的鉴定

Identification of Elements and Transcription Factors for ezrin Basal Transcriptional Activity in Lung Cancer Cells

通过一系列信号传递过程最终激发转录因子，转录因子与顺式作用元件结合，激活形成RNA聚合酶Ⅱ转录复合物，从而启动基因的转录表达。

After a series of signal transmission , transcription factors was activated to combine with the cis-element , and then stimulated to form a transcription complex of RNA polymerase ⅱ, which initiates gene transcription .

KEGG及其在顺式作用元件预测中的应用

KEGG and application in the predication of CIS - acting element

但对于Ⅰ型胶原基因启动转录的机制，细胞因子如何在启动转录水平激活靶基因转录，瘢痕成纤维细胞中纤维化相关DNA结合蛋白如何与其顺式作用元件相互作用等均不清楚。

However , many are not clear about the transcriptional mechanism of type I collagen gene , activation of target genes in transcription by cytokines , and interaction of DNA-binding proteins related to fibrosis with their cis-acting elements in pathologic scars .

分析发现该基因的启动子上存在很多与光、冷、热、旱、盐胁迫和ABA响应相关的顺式作用元件。

Bioinformatics analysis showed that the promoter of the gene had many cis acting elements related stress such as light , cold , hot , drought , salt and ABA .

CBF通过与CRT/DRE（C-repeat/Dehydrationresponsiveelement）顺式作用元件的结合激活低温和脱水响应基因的表达，提高植物的抗逆性。

CBF transcriptional factor passes with CRT / DRE ( C-repeat / Dehydration responsive element ) syn-form functional element union activation low temperature and dehydrated response gene expression , Enhances the plant the resistance .

它编码病毒诱导的DNA聚合酶，能与其它复制因子一起与杆状病毒DNA的同源区和非同源区的顺式作用元件相互作用起始DNA复制。

It can encode DNA polymerase induced by virus . In the process of replication , DNA polymerase can bind to homologous regions and non - homologous regions , which are believed to act as the origins of virus DNA replication with other replication factors .

VCH3（-276）GUS（无SA顺式作用元件）嵌合基因驱动的GUS酶活性最低，仅仅稍高于对照。

VCH3 ( - 276 ) GUS ( not containing SA cis-motif ) directed the lowest GUS activity , only a little higher than CK .

对BrpFLC2启动子进行生物信息学软件分析表明，BrpFLC2启动子包含了多个TATA-box，CAAT-box，以及其他顺式作用元件。

It contains several TATA-box , CAAT-box and other cis-acting elements .

运用互为反向的Lox位点，在鼠红白血病MEL细胞中进行靶载体的整合和交换载体的交换，探讨在特定的染色质环境下红系特异性顺式作用元件的功能。

Here , site-specific integration with inverted Lox sites was used to investigate the erythroid cis - acting DNA element in specific chromatin contexts in mouse erythroleukemia cells .

用PlantCare软件进一步作顺式作用元件分析，发现两段启动子序列包含大量光反应元件及脱落酸、赤霉素、水杨酸、生长素、乙烯等激素应答元件。

After this , we analysised the upstream regulatory elements in the two promoter fragment with software of Plant Care , and found lots of light - responsive element , and abscisic acid-responsive element , auxin - responsive element , ethylene-responsive element , gibberellin-responsive element , salicylic acid-responsive element .

通过启动子报告基因实验以及EMSA（Electrophoreticmobilityshiftassay）实验等，我们证实RIG-G基因启动子区的ISREI和ISREII两个顺式作用元件是该基因转录表达的分子基础。

By luciferase reporter assay and electrophoretic mobility shift assay ( EMSA ), we confirmed that ISRE I and ISRE II are the molecular basis of RIG-G gene transcription .

应用软件分析预测WNK4基因启动子区顺式作用元件。

And certain softwares were used to analyze the cis-acting elements within the WNK4 promoter .

EGR－1的启动子为早期生长反应因子－1基因上游约－550～0bp的一顺式作用元件，其活性受控于一些诱导剂，如电离辐射。

EGR-1 promoter , a radiation-inducible promoter , is the cis-element ( - 550-0bp ) of the early growth response-1 gene , its activity is regulated by inducers . such as ionizing radiation .

CMO基因的表达受其上游启动子及转录因子的调控，转录因子与启动子上的顺式作用元件相互作用，促进下游基因的表达。

CMO gene expression is regulated by upstream promoter and transcription factors . The interaction between transcription factors and promoter cis-acting elements promote the expression of genes downstream .

DRE顺式作用元件能与DREB转录因子特异结合，在诱导逆境（干旱、高盐、低温）基因表达过程中起重要作用。

DRE cis-acting element which could specifically bind DREB transcription factors plays important roles in inducing gene expression involved in dehydration , low-temperature , and high-salinity stresses .

它们可以与GCC-box、DRE/CRT等顺式作用元件结合，参与生物和非生物胁迫响应。

They can be combined with the GCC-box , DRE / CRT cis-acting elements involved in biotic and abiotic stress response .

许多调节细胞生长，细胞形状，细胞运动，细胞命运以及细胞通讯的基因通常在它们的顺式作用元件上拥有AP-2结合位点。

A number of genes that mediate cell growth , cell shape , cell movement , cell fate and cell communication frequently possess the AP-2 binding site in their cis-regulatory sequences .

CBF（C-repeatBindingFactor）基因能够识别并与COR基因启动子区域的CRT/DRE（C-repeat/Dehydrationresponsiveelement）顺式作用元件特异结合，诱导其表达，从而提高植物的抗寒能力。

CBF ( C-repeat binding factor ) gene recognize and specifically bound with cis-acting element of CRT / DRE ( C-repeat / dehydration responsive element ) in COR gene promoter region , induce the expression of CBF gene , thereby enhancing the ability of the cold resistance .