多聚腺苷酸化

真核生物前体RNA需要经过一系列的转录后加工才能形成成熟的mRNA，这些加工过程包括选择性剪接、RNA编辑以及RNA3'端多聚腺苷酸化等过程。

Before maturing of the eukaryotic mRNA , Pre-mRNA undergoes several processes of post-transcriptional modification , including alternative splicing , editing and polyadenylation .

结果多聚腺苷酸化信号缺陷病毒可以在PA317病毒包装细胞形成病毒颗粒；

Results polyadenylation signal-deficient retroviruses could be packaged by PA317 packaging cells .

方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；

Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells .

目的通过人工改变多聚腺苷酸化信号的重组逆转录病毒感染人胃上皮细胞GES-1，检测病毒转录能否产生病毒-宿主融合转录产物及其是否具有转化胃上皮细胞GES-1的能力。

Objective To verify if mutated polyadenylation signal retroviruses can produce viral-host readthrough transcripts ( Rth ) and have the ability to transform human gastric epithelial GES-1 cells , and to discuss the new functions of retroviruses in gastric cancer related gene research .

目的：通过人工改变逆转录病毒多聚腺苷酸化信号，检测病毒转录能否产生病毒宿主融合转录产物及其是否具有转化细胞的能力，探讨病毒致癌的机制。

Objective : To check if mutated polyadenylation signal retroviruses can produce viral host readthrough transcripts ( Rth ) and have the ability to transform normal REF 1 cells .