间隔区

方法对22株申克孢子丝菌和12种12株暗色真菌临床分离株的核糖体DNA（rDNA）内转录间隔区（ITS）进行聚合酶链反应扩增。

Methods Species-specific primers were used to amplify the internal transcribed spacer region 2 of rDNA of 22 clinical isolates of Sporothrix schenckii and 12 strains of dematiaceous fungi .

蓝藻OscilatoriaceaerDNA16S-23S基因间隔区的序列分析及其分类学意义

Sequence Analyses of rDNA Intergenic Spacer Region from Oscillatoriaceae ( Cyanobacteria ) and Its Taxonomic Significance

核糖体DNA的内转录间隔区序列标记在真菌分类鉴定中的应用

Application of Ribosomal DNA Internal Transcribed Spacer in Fungi Taxonomy

我国不同地区白木香核糖体DNA内转录间隔区碱基序列分析

Ribosomal DNA ITS sequence analysis of Aquilaria sinensis from different geographical origin in China

在植物学研究中，人们越来越多地采用核糖体DNA内转录间隔区（ITS）作为分子标记。

In the botany study , more and more people us ribosomal DNA internal transcribed spacer ( ITS ) as a molecular marker .

不同绿藻核rDNA间隔区ITS的序列和系统发育分析

An Analysis of Nuclear rDNA ITS Sequence and Phylogenesis of Different Green Algae

核糖体RNA基因间隔区ITS及IGS在真菌分子生物学鉴定和分型中的应用

Application of ribosomal RNA gene ITS and IGS regions in the molecular identification and classification fungi

rRNA基因间隔区序列用于亲缘关系密切的根瘤菌种群鉴定及系统发育分析

Identification and phylogenetic analysis of closely related rhizobium species by rRNA gene intergenic spacer sequence

结论应用16S～23Srdna间隔区序列PCR和RFLP分析能将结核分枝杆菌复合菌群等几组常规方法难鉴定的菌群加以区别。

Conclusion PCR and RFLP analysis of 16S-23S rDNA spacer sequence can differentiate several mycobacterium complex quickly .

结果表明，rRNA基因间隔区序列能很好地区分种间亲缘关系十分密切的菌株。

The results indicated that sequences of rRNA gene intergenic spacer would clarify the closely related species .

大肠埃希氏杆菌16S&23SRRNA基因间隔区片断分析在粪便污染源示踪上的应用

Use of 16S-23S rRNA Intergenic Spacer Region PCR Analyses of Escherichia Coli Isolate to Identify Fecal Sources

16S～23Srdna内转录间隔区序列分析及其在分支杆菌鉴定中的应用价值

16S ~ 23S rDNA internal transcribed spacer sequence analysis and its application in mycobacterial identification

位于核基因具有多拷贝的内转录间隔区（Internaltranscribedspacer，ITS），已广泛用于种内和种间亲缘关系分析。

Located in nuclear genes with multiple copies of the internal transcribed spacer , has been widely used in intraspecific and interspecific phylogenetic relationship analysis .

产生伴胞晶体的芽胞杆菌CTC菌株的16S-23SRRNA间隔区分析

Analysis of 16S - 23S rRNA Space Region of Parasporal Crystal Forming Bacillus strain CTC

核糖体DNA内部转录间隔区（Internaltranscribedspacer，ITS）作为线粒体DNA信息的补充，在昆虫学的研究中越来越受到重视。

As an important complement of the information obtained from mtDNA , the internal transcribed spacer ( ITS ) of nuclear ribosomal DNA is being increasingly applied in entomological study .

中华绒螯蟹（Eriocheirjaponicasinensis）内转录间隔区1（ITS1）大小变异的研究（英文）

Study on Size Variation of rDNA Internal Transcribed Spacer 1 in Eriocheir japonica sinensis ( Decapoda , Grapsidae )

结论16S23Srdna间隔区序列PCR扩增及DNA探针杂交在基因水平上确认引起此次注射后暴发感染的致病菌为偶然分支杆菌，该方法具有灵敏、特异的特点。

Conclusion The results showed that the pathogen of infection outbreak were M.fortuitum . The 16S-23S rDNA PCR investigative system is sensitive and specific , which can identify M.fortuitum at gene level .

三株赤潮硅藻5.8Srdna及转录间隔区（ITS）的克隆及序列分析

The cloning and sequence analysis of the 5.8S rDNA and its region from three strains of marine diatoms

对白色红曲菌、火红色红曲菌、烟灰色红曲菌和橙色红曲菌各自固有的核糖体内转录间隔区（ITS）rDNA序列和生物化学特性进行了研究。

Q. Li & F. Guo were studied with their respectively intrinsic rDNA sequence and biochemical characteristics of internal transcription silent region ( ITS ) in ribosome .

目的研究以16S-23Srdna内转录间隔区（Internaltranscribedspacer，ITS）序列为靶基因设计分支杆菌菌种寡核苷酸探针，应用PCR-反向杂交技术快速鉴定分支杆菌菌种。

Objective To study the rapid identification of mycobacterium species by PCR-reverse hybridization assay using oligonucleotide probes based on 16s-23S rDNA internal transcribed spacer ( ITS ) sequences .

5'端非编码区（NR）长120nt，基因间隔区（IR）长296nt，3'端NR区含301个碱基。

The non coding regions ( NR ) located at 5'terminal and 3'terminal were 120 nt and 301 nt , respectively , and the intergenic region ( IR ) was 296 nt long .

在无脊椎动物的分子系统学研究中，作为对线粒体DNA信息的重要补充，人们越来越多地采用核DNA的核糖体内转录间隔区（ITS）作为分子标记。

The internal transcribed spacer ( ITS ) of the nuclear ribosomal DNA , as an important complement of information obtained from mtDNA , is being used increasingly to construct or reconstruct phylogenetic relationships among species and to distinguish morphologically similar species in invertebrates .

通过vectorNTI8软件进行多序列比对后，针对曲霉菌rDNA基因的转录间隔区ITSI设计引物，可特异扩增出多种曲霉菌菌株。

Through the compare of the Vector NTI 8 software for multi-sequences alignment , the target gene in Aspergillus rDNA transcribed spacers ITS I designed primers , specific amplification can produce a variety of Aspergillus strains specially .

真菌核糖体DNA内转录间隔区（ITS）在进化上比编码区快，种内的不同菌株之间高度保守，但在种间变化极大，故可为真菌学的研究提供丰富的遗传信息。

Among them , ribosomal DNA internal transcribed spacers ( ITS ), evolves faster than coding sequence region , is highly conservative within species , but with great difference between species . Thus , it can provide abundant genetic information for the research of mycology .

结论：不同植物性中药材种子rRNA基因内转录间隔区碱基序列可做为从分子水平进行鉴定的标记。

Conclusion : The base sequences of the internal transcripted spacer of rRNA gene in different plant Chinese seeds can be applied to be identifying marker in molecular level .

[方法]先从并殖吸虫病患者痰中分离出虫卵，然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因（ITS2），并直接用于测序从而获得该基因的核苷酸序列。

[ Methosd ] The complete nuclear ribosomal DNA second internal transcribed spacer ( ITS2 ) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

鸡毒霉形体的分离鉴定及其16S/23SRRNA基因间隔区、TM-1基因序列差异分析

Isolation and Identification of Mycoplasma Gallisepticum and Difference Analysis of Sequence of MG 16S / 23S rRNA Intergenic Spacer Region and TM-1 Gene

方法：PCR扩增红色毛癣菌核糖体的非转录间隔区（NTS）的两个串联重复亚单位TRS1和TRS2区，进行种内分型。

Methods : Two novel tandem repeat subelements ( TRSs ), TRS-1 and TRS-2 , located in the Trichophyton rubrum rDNA nontranscribed spacer ( NTS ) were amplified from 63 strains of Trichophyton rubrum .

目的比较我国云南、海南省、广西壮族自治区及泰国等不同地区微小按蚊核糖体DNA第2内转录间隔区（ITS2）序列差异。

Objective To compare the sequence difference of the ribosomal DNA second internal transcribed spacer ( ITS2 ) in Anopheles minimus from Yunnan , Hainan , Guangxi of China and from Thailand .

目的利用16s-23SRRNA间隔区（ITS）的多态性，对中国布鲁氏菌种间或种内生物型进行鉴别，评价ITS作为基因标识物的意义，寻找适合布鲁氏菌分型研究的基因标识物。

Objective To identify Brucella spp by 16s-23s rRNA Ribosomal-Spacer-Region ( ITS ) and to estimate the significance of ITS as gene labelling in Brucella .