结果压力蒸汽灭菌法不管是延长灭菌时间、还是采用间歇灭菌法均无法达到100%的灭菌效果；
RESULTS Pressure steam sterilization was unable to achieve 100 % sterilizing effect , whether we extended the time or use the intermittent ( sterilization ) .
间歇灭菌法