酶钝化

另外，微波前处理可以增加芦笋热水温烫处理过程中维生素C降解的活化能并且减小过氧化物酶钝化的活化能。

In addition , microwaves pre-treatment could increase the activation energy of ascorbic acid degradation and decrease the activation energy of peroxidase inactivation during water blanching of asparagus .

将传热方程与酶钝化反应动力学方程耦合，得到了BSA相对残余活性A/A0的分布。

Coupling with the kinetics equation of the inactivation of enzymes , the distribution of the relative activity retention is obtained .

高压脉冲电场（PEF）是一种非热杀菌技术，它的主要用途在于对热敏性物料的杀菌、酶钝化等方面。

Pulsed electric fields ( PEF ) is an emerging non-thermal processing technology used for thermosensitive materials stabilization and enzyme inactivation .

高压CO2技术作为一种新型的非热力杀菌技术引起了重视，本文主要介绍了高压CO2技术对微生物的杀灭效果和对酶钝化作用的研究进展及其相关机理。

As a novel non-thermal pasteurization method , high pressure CO2 has become the international research focus . The inactivation of microorganisms and enzymes by high-pressure CO2 and their mechanisms are introduced in this article .

蒜酶钝化后，进行蒜氨酸的浸提，较佳的佳浸提条件为：乙醇浓度60%，温度50℃，时间2h，固液比1：5（g／ml）。

The optimal conditions for extraction of alliin was achieved when the garlic was mixed with 60 % ethanol of the extration solution in the ratio of 1:5 ( g / mL ) and extracted for two hours at 50C .

PEF技术主要应用于生化杀菌、酶钝化处理及榨汁、果蔬渗透脱水和干燥的前处理。

PEF technology is mainly used in the process of bio-chemical sterilization , enzyme passivation and the pre-treatment of juice extra , osmotic dehydration and drying of fruits and vegetables .

酶钝化豆类种子胰蛋白酶抑制子的研究

Research of Legumes Seeds Trypsin Inhibitors Inactivated by Enzyme

利用该模型，考察了高压釜内食品填充率对酶钝化均匀性的影响。

The effect of the filling ratio of food in the pressure vessel chamber on the enzymes inactivation A / A0is studied with the model .

韭菜样本用微波炉加热处理，使酶钝化消除含硫基质干扰，然后用乙腈提取、逆固相分散净化，用反相高效液相色谱-二极管阵列检测器检测。

Then the pretreated samples were extracted with acetonitrile , further cleaned up by reverse solid phase dispersion , and analyzed by reversed phase HPLC with diode array detector ( DAD ) .

高填充率可以获得更高的酶钝化效果，但样品中A/A0的分布更不均匀；对多个琼脂样品处理，则使各个样品之间差异变小。

High level of enzymes inactivation is obtained by the high filling ratio , but it gets more nonuniform . As for the multi-packs processing , the difference between the samples becomes smaller .

采用气相色谱及质谱联用技术，测定葱、蒜及韭菜等蔬菜中多种农药残留量时，在粉碎试样之前，用磷酸或微波加热处理试样，可使蒜酶钝化并消除其基体干扰。

In the determination of residual pesticides in onion , garlic and chives by GC-MS , the sample was treated with phosphoric acid or with microwave before crushing , to make the allinase inactive and to eliminate the matrix interference .

施加压力对三种酶的钝化作用呈极显著水平（P＜0.01）。

Pressure treatment had an extreme significant effect ( P < 0.01 ) on inactivating these enzymes .

结果表明PEF可以钝化PE的活性：随脉冲电场电场强度的增强（5～25kV/cm）和脉冲数的增加（207～1449个）PE酶活钝化效果增强；

The results showed relative activity of PE decreased with the increase in applied electric field intensity ( 5 ~ 25 kV / cm ) and pulse numbers ( 207 ~ 1449 ) .

在微波400W的处理下，等质量不同厚度香蕉片该酶完全钝化的时间为45s。

When such banana masses were treated by 400W of microwave , their complete deactivating time were 45 seconds .

脉冲强光杀菌对酶的钝化作用研究

Study on the effect of pulsed light on enzyme

一个非竞争性抑制剂的净结果相当于转化某些酶成为钝化形式。

The net result of a non-competitive inhibitor is equivalent to converting some of the enzyme present to an inactive form .

从得到的差异表达的蛋白点可知本研究所涉及的耐药机制包括灭活酶或钝化酶的产生，目标靶蛋白的修饰，主动药物转运系统的变化以及细胞外膜的通透性的改变等几种。

From differentially expressed protein spots , antibiotic-resistant mechanism of V. alginolyticus of which in activator or inactive enzyme were produced , target proteins were modified , efflux pump systems and permeability of outer membrane were changed could be known .

细菌对诺氟沙星耐药性的形成可能与细菌外膜通透性改变从而减少抗生素的摄入以及产生灭活酶或钝化酶修饰抗生素有关.本研究为进一步揭示嗜水气单胞菌外膜蛋白与耐药性相关性提供了理论依据。

The change of outer membrane permeability and the generation of inactive enzyme or modifying enzyme might be the reason that bacteria have the resistance to norfloxacin . The research was aim to know more about the correlation between A. hydrophila outer membrane protein and the resistance .

结果表明，鸡腿菇PPO活性的最适pH值4.0~5.0，最适温度20℃，在沸水浴30s后酶基本被钝化。

The results showed that the optimal pH and temperature of PPO activity were pH 4.0-5.0 and 20 ℃ respectively , and the PPO was inactivated at 100 ℃ for 30s .

棕色固氮菌中固氮酶的氧钝化及其保护

The oxygen inactivation and protection of Azotobacter vinelandii Nitrogenase

酒花能对微生物体内的酶起药物钝化、改变目的底物、抑制药物流入等作用。

Besides , the hops had the functions of inactivating enzymes in microbial body , changing goal objects , and inhibiting the inflow of medicine etc.

当然，食品热处理的理论根据之一就是使食品中的酶变性和钝化，从而使食品不再继续受到酶的作用。

Of course , one basis for heat processing is to denature and inactivate enzymes so that the food is not subjected to continuing enzymes activity .

研究发现，强脉冲磁场对多酚氧化酶有微弱的钝化作用，对草莓过氧化物酶有较强的钝化作用。

It was found that the high pulsed magnetic field had a weak inactivation effect on the polyphenol oxidase and strong inactivation effect to peroxidase .

微波加热对微生物和酶的热力效应，目前已深入到对杀菌对象不同种类微生物的致死和酶的钝化作用机理研究，而非热力效应还难以量化。

Studying inactivation of different targets by microwave treatments has proved thermal effect of microwave heating on microorganisms and enzymes , while the nonthermal effect is still difficult for precise measurement .