酶联免疫检测

方法：采取酶联免疫检测仪测定50例青年人脑卒中患者LP（α）、oxLDL含量，并与50例正常对照者进行比较。

Methods : Serum content of LP (α) and oxLDL were determined by ELISA way in the young stroke group ( 50 cases ) and the result was compared with that of control group ( 50 healthy people ) .

方法采用丙型肝炎病毒核心区及NS4区合成寡肽建立酶联免疫检测方法，对血清中丙型肝炎病毒抗体进行分型。

Methods The synthetic peptides deduced from the putative Core and NS4 region were used to set up the ELISA system for dividing the HCV antibodies into two types .

以此抗体为基础，设计了间接竞争两步酶联免疫检测方法，并优化了缓冲液浓度、pH、吐温20浓度等参数。

Based on this purified antibody , an indirect competitive ELISA was developed .

PCR产物酶联免疫检测法在临床检验中应用

Application of PCR-EIA Method in Clinical Detection

人巨细胞病毒IgG酶联免疫检测试剂盒的研制

Preparation of ELISA Kit for Detecting Cytomegalovirus IgG

本文首先对酶联免疫检测技术（ELISA）做了简要介绍。

In this paper , the technology of Enzyme-Linked Immunosorbent Assay ( ELISA ) was briefly introduced .

酶联免疫检测甲型肝炎IgM抗体特异性诊断及临床特点

IgM Specific Diagnosis and Clinical Features of Enzyme-complex Immunologically Detected Hepatitis A Virus

方法用ELISA酶联免疫检测法定量分析MM和浆细胞白血病患者血浆syndecan1水平。

Methods Serum level of soluble syndecan 1 of patients with MM and plasma cell leukemia was determined by ELISA .

用酶联免疫检测技术对慢性肝病患者血清Ⅰ型胶原及TNFα进行了检测。

Serum levels of type I collagen and TNF α were determined in patients with chronic liver disease by enzyme-linked immunoassay .

EPO酶联免疫检测方法学的建立与临床应用研究

Development of ELISA Method for Erythropoietin and Study on the Clinical Application

结论伤寒杆菌H抗原酶联免疫检测快速、特异、敏感为实验室检测的首选方法。

Conclusions : Enzyme-linked immunoassay for detection of typhoid bacilli H antigen was the first selective method for laboratory examination of typhoid because of its fast , specificity and sensitivity .

流式细胞仪检测血小板P-选择素（CD62p），酶联免疫检测血浆假血友病因子（vWF）。

To detect CD 62p with flow cytometry , and detect vWF with ELISA .

结果表明：利用酶联免疫检测技术可检测到巴西橡胶树胶乳中含有较多的IAA，iPAs，ABA。

The results indicated that IAA , iPAs and ABA were abundant in the latex .

采用猪视网膜为靶组织，采用FDA酶联免疫检测的样品前处理方法进行了盐酸克伦特罗残留的定性及定量检测方法研究。

The qualitative and quantitative determination of Clenbuterol residue in porcine retinal tissue by the method of FDA was studied .

结论双单抗夹心酶联免疫检测HIV－1p24抗原方法敏感、操作简便，可用于临床HIV感染的辅助检测手段。

Conclusion This Sandwich EIA kit constructed for detection of HIV 1 p24 antigen was sensitive , convenient and could be helpful in clinical detection of HIV infection .

和琼脂糖凝胶检测方法相比，8例杂合子样本的酶联免疫检测结果，其阳性和阴性信号的对比结果更为明显，表明ELISA显色具有更高的灵敏度，同时可实现更大的通量。

The result of ELISA detection for eight heterozygote sample had more obvious comparision of positive and negative signal compared to the result of agarose gel electrophoresis .

方法该文采用细菌培养、肥达反应、伤寒杆菌H抗原酶联免疫检测检查52例伤寒患者，20例正常人，进行对照观察。

Methods : We examined and observed 52 patients with typhoid and 20 normal controls by bacterial culture , Widal reaction , and enzyme-linked immunoassay for detection of typhoid bacilli H antigen .

结果在59例结核病患者痰标本中，国产PCR-酶联免疫检测试剂盒与PCR荧光试剂盒分别检出26例阳性和24例阳性，国外试剂盒检出24例阳性；

Results In 59 TB samples , 26 and 24 samples were positive respectively by the homemade PCR-ELISA and PCR-fluorescence kits , and 24 samples were positive by the import kit .

通过酶联免疫检测仪（MTT）测定软骨细胞存活数，流式细胞仪测定软骨细胞分裂增殖百分数。

The survival number of chondrocytes was investigated by MTT enzyme-linked immunometric meter and the splitting proliferation percentage of chondrocytes was determined by flow cytometer .

建立了ABA间接酶联免疫检测法（ELISA）。

An indirect method of enzyme linked immunosorbent assay ( ELISA ) was established by utilizing ABA-OA conjugates for coating wells , on which rabbit anti-ABA sera reacted .

用两种抗粒细胞集落刺激因子（G－CSF）单克隆抗体，本院自制了G－CSF酶联免疫检测试剂盒。

By adding monoclonal antibodies from BALB / c mice to the granulocyte colony-stimulating factor ( G-CSF ), we established a sandwich ELISA for detecting G-CSF levels in human serum samples .

转Bt基因植物中杀虫蛋白的酶联免疫检测技术研究Ⅱ.Bt杀虫晶体蛋白抗体的制备

Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants ⅱ . Preparation of Polyclonal Antibody to Bt Insecticidal Protein

将纯化的衣壳蛋白免疫新西兰大白兔，制备抗血清，并通过间接ELISA方法对抗血清效价进行测定。结果表明该抗血清效价可达到1：10000，符合酶联免疫检测的要求。

The capsid protein was used to immune New Zealand rabbit with the antiserum prepared . The titer of the antiserum was 1:10000 as was determined with indirect ELISA ( enzyme linked immunosorbent assay ) .

利用酶联免疫检测技术在巴西橡胶砧穗胶乳中检测到了三种内源激素：生长素IAA、细胞分裂素iPAs和脱落酸ABA。

Three kinds of endogenesis phytohormones in latex of rootstock and scion of Hevea Brasiliensis were tested by using PGRIA : IAA , iPAs , ABA .

方法体外培养人脐静脉血管内皮细胞，经缺氧及血管活性物质处理，Northern杂交、酶联免疫检测和计算机图像分析等观察vEGFmRNA和蛋白表达水平。

Methods Culture the human umbilical vein endothelial cells and then treat by hypoxia and vascular activators . The expression on VEGF mRNA level and protein level were assayed by northern blots , ELISA and computer figure analysis system respectively .

转基因抗虫作物中豇豆胰蛋白酶抑制剂（CpTI）酶联免疫检测方法的建立

Development of an Enzyme Immunoassay for the Determination of the Cowpea Trypsin Inhibitor ( CpTI ) in Transgenic Crop

选用生育期不同的3个品种，采用酶联免疫检测技术（ELISA），研究了开花前和开花后光周期处理对大豆叶片内源激素含量及其平衡状况的影响。

Three representative varieties belonging to different Maturity Groups were chosen and the ELISA was used to study the photoperiodic effects on the endogenous hormonal amount and their balance in soybean leaves before and after flowering ( R1 ) .

应用磁性分离酶联免疫检测技术（MAIA）对210名正常非妊娠妇女的血清进行HCG定量测定，平均值为3549mIU/ml血清。

The human chorionic gonadotropin ( HCG ) of 210 normal non-pregnant women was tested using magnetic antibody immunity assay ( MAIA ), the average value was 3.549mIU/ml .

为探讨类风湿关节炎（RA）的发病机理，给临床诊治提供实验依据。采用流式细胞术和酶联免疫检测技术，分析RA患者外周血淋巴细胞亚群和IL4、IL6水平变化。

In this paper some peripheral blood lymphocyte subsets using Flow Cytometry as well as IL-4 and IL-6 levels by Enzyme-linked immunoassay were detected to study and discuss pathogenesis of RA and obtain experimental basis for clinical diagnosis and treatment .

心肌肌钙蛋白I-C复合物酶联免疫检测方法的建立

Development of Enzyme Linked Immunosorbent Assay for Detection of Cardiac Troponin I-C Complex in the Diagnosis of Cardiac Damage