蛋白质纯化

反胶束用于蛋白质纯化和生物转化的研究进展

Advances of Protein Purification and Bioconversion in Reversed Micelle Solvents

重组蛋白质纯化技术

The Progress of Recombinant Protein Purification Techniques

鼠疫耶尔森氏菌F1-V基因克隆、表达、蛋白质纯化以及免疫保护性的初步研究

Cloning , Expression and Purification of Yersinia Pestis F1-V Fusion Protein in Escherichia Coli and Studies Immune Protection in BALB / c Mice

一种蛋白质纯化流程用于提纯大肠杆菌表达的蛋白片段

A Procedure for Purification of Protein Fragments Expressed by Escherichia coli

重组蛋白质纯化中切割蛋白酶的选择与应用进展

Application and Selection of Cleavage Protease in Purification of Recombinant Protein

最后将所提的RS-ID3算法用于蛋白质纯化工艺摸索，实例验证也具有很好的效果，该方法为纯化方法的确定提供了支持。

Support is provided for the purification methods of determining .

2种棉花叶片总蛋白质纯化方法比较研究

Comparison of Two Purification Methods of Cotton Leaf Total Protein

近年来开发的多种非盐依赖层析介质与方法，都已在蛋白质纯化中得到应用。

The recent development of the new ligands and methods on salt-independent chromatography are summarized .

结论本研究建立的血清蛋白质纯化技术，可有效应用于疾病的血清蛋白质组学研究。

Conclusion The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases .

球形纤维素/钛白粉复合扩张床吸附剂的制备及其在蛋白质纯化中的应用

Preparation of Spherical Cellulose / TiO_2 Composite Adsorbent for Expanded Bed Adsorption and Its Application to Purification of Proteins

将有机溶剂抗溶剂与高压气体等电沉淀技术结合起来，提出了一种新的蛋白质纯化方法。

A novel method for protein purification was presented , which was the integration of organic antisolvent and compressed gas isoelectric precipitation .

现代生物技术的发展，尤其是蛋白质纯化技术及基因分子生物学技术的发展大大地推动了血友病甲的预防、诊断和治疗。

The development of modern biotechnology , especially of protein purification and gene molecular biology technology , has greatly prompted the prevention , diagnosis and treatment of hemophilia A.

层析是蛋白质纯化的关键技术之一，作为层析技术的核心&层析介质一直以来是层析技术研究的一个热点。

Chromatography is one of the most important purification techniques of proteins . The attention of chromatographers has hitherto focused on media which is regarded as the key for chromatography technology .

方法通过对培养中收集的大量条件培养基进行超滤、层析、透析等截留相对分子质量8～10ku的蛋白质纯化，获取19个层析柱。

Methods The active peptide with 8 ~ 10 ku molecular weight in the conditioned medium obtained from the cultivation of human osteosarcoma cells were partially purified by ultrafiltration , chromatography and dialysis .

本文阐述了凝胶过滤、离子交换和疏水相互作用色谱的基本原理和操作条件，说明了它们在蛋白质纯化中的应用策略。

The basic principles and operation considerations of gel filtration chromatography , ion exchange chromatography and hydrophobic interaction chromatography were mainly clarified , and their applying strategies in separation and purification of protein were illustrated .

冈此，目前磁性微球主要应用于蛋白质纯化、细胞分离、酶固定、环境/食品微生物检测等领域。

It will quickly move or separate in external magnetic field , therefore magnetic polymer microspheres show important practical applications in fields of protein purification , cell separation , enzyme immobilization and microbe of environment / Food testing , et al .

一种具有抑制糜蛋白酶活性的血清DNA结合蛋白质的纯化和理化特性

A serum DNA binding protein with chymotrypsin inhibition activity & purification and characterization

疏水作用色谱法（HIC）是一种有效的蛋白质分离纯化方法。

Hydrophobic interaction chromatography ( HIC ) is an attractive method for protein purification .

类弹性蛋白（elastin-likepolypeptide，ELP）一种新型的重组蛋白质分离纯化标签。

Elastin-like polypeptide ( ELP ) is a new recombinant protein purification tag .

ColiBL21（DE3）中表达相对分子质量约为28000的蛋白质，纯化后，COMT蛋白的纯度大于90%。

Coli BL21 ( DE3 ), through puring , the purity of COMT protein can be more than 90 % .

目的：利用蛋白质分离纯化技术提取末端脱氧核苷酰转移酶（TdT），以研究其分子组成、理化性质，探讨其与白血病之间的联系。

Objective : Isolation of terminal deoxynucleotidyltransferase ( TdT ) for studying its molecular constitute , physical and chemical properties , and its relation to leukemia .

结论应用镍亲和层析的方法纯化融合蛋白时，His-tag在融合蛋白中的位置对蛋白质的纯化非常重要，His-tag可以在融合蛋白的N端和C端，但不能在中间。

Conclusion When fusion protein is purified by Ni2 + affinity chromatography , the position of 6-His tag is very important for the purification of fusion protein . 6-His tag could be placed at the amino terminal and carboxy terminal of fusion protein except the middle .

离心超滤技术适合放射性碘标记蛋白质的纯化。

Ultrafiltration is suitable for purification of the radioiodine labeled protein .

蛋白质分离纯化与层析技术进展

Separation and purification of protein and recent advances in chromatography

蛋白质分离纯化和蛋白质结晶的研究方法

Studies on separation and purification crystallization of protein

光敏核不育水稻61kD特异性蛋白质的纯化和N-端序列分析

Analysis on amino-terminal sequence and purification of a 61 kD protein from Photoperiod Sensitive Genic Male-sterile Rice

硫脲修饰交联壳聚糖制备工艺简单，吸附容量较大且容易洗脱，可用作蛋白质分离纯化的吸附剂。

The thiourea modified crosslinked chitosan resin can be applied to the separation and purification of proteins .

蛋白质分离纯化是蛋白质产品工业化生产的关键之一。

One of the cruxes of the industrial scale production of protein is its separation and purification , the characters and key problem of which are analyzed .

蛋白质分离纯化技术推动了对基因工程、分子生物学、免疫学和疾病诊断学等许多新兴学科的发展。

Protein separation and purification technology plays an important role in promoting the development of genetic engineering , molecular biology , immunology , disease diagnostics , and many other emerging disciplines .

综述了近年来甲壳素及壳聚糖衍生物吸附剂、色谱填料、分离膜及医用免疫吸附剂在蛋白质分离纯化中的应用。引用文献65篇。

The application of chitin and chitosan derivative adsorbents , chromatography packings , separation membranes and immunoadsorbent in the separation and purification of proteins is reviewed in the present paper with 65 references .