荧光素酶报告基因

人Puma启动子荧光素酶报告基因的构建和鉴定

Construction and identification of human Puma promotor luciferase report gene vector

荧光素酶报告基因在转基因蚕中的应用

Application of Luciferase Report Gene in Transgenic Silkworm

应用荧光素酶报告基因技术检测DHMEQ对NF-κB基因活性的影响；

Transcriptional activity of NF - κ B was determined using luciferase reporter gene assay .

利用荧光素酶报告基因检测技术确证ERδ5对ERα转录活性的影响。

The effect of ER δ 5 on ER transcription was investigated by luciferase reporter assay .

用双荧光素酶报告基因检测IL-8激活程度。

IL-8 gene promoter activation was detected using dual luciferase reporter gene assay .

我们构建了包含FLOT2启动子和Wnt信号通路转录因子结合位点的荧光素酶报告基因载体。

We generated a luciferase reporter construct containing the Wnt signaling pathway transcription factor binding sites .

采用瞬时转染的方法，通过荧光素酶报告基因实验检测eNOS基因转录起始点上游长16kb启动子区域的活性。

Promoter activity of eNOS gene was determined by luciferase reporter gene assay .

构建TWIST的报告基因载体，利用双荧光素酶报告基因实验检测在缺氧条件下或共转染HIF-1α真核表达质粒时，TWIST启动子活性的变化。

The TWIST reporter gene vector was constructed ; The TWIST promoter activity was evaluated by dual luciferase reporter assay when co-transfected with HIF-1 or under hypoxia condition .

但是激素和细胞因子对淋巴细胞中GH表达是否有调节作用呢？我们用荧光素酶报告基因的方法，探讨了这个问题。

There are few reports about the effects of hormones and cytokines on lymphocyte GH gene transcription . We addressed this question with the method of luciferase reporter gene .

目的克隆编码mdr1基因的启动子并插入荧光素酶报告基因载体。

Objective To clone the promoter of multi-drug resistance 1 gene and insert it into a luciferase reporter gene vector .

运用生物信息学分析miRNAs对eNOS的潜在结合位点，荧光素酶报告基因检测miRNAs对其靶标的直接作用。

The potential binding sites of miRNAs in eNOS were analyzed by bioinformatics software . The direct inhibition on eNOS was tested by luciferase reporter assay .

目的构建磷脂酰肌醇蛋白聚糖3（GPC3）启动子荧光素酶报告基因载体，并验证其转录活性。

ObjectiveTo construct glypican-3 ( GPC3 ) promoter luciferase reporter gene vector , and to analyze its transcriptional activity .

结果：成功构建了6种含有不同长度ICAM-1基因启动子序列以及启动子中NF-κB和Sp-1位点突变序列的荧光素酶报告基因质粒。

The relative luciferase activities were detected in the transfected HUVECs . RESULTS : Series of luciferase reporter gene containing different sequences of human ICAM-1 promotor and site-directed mutants of NF - κ B and Sp-1 in promotor were successfully constructed ;

转染BCR-ABL嵌合基因两种不同的方法，通过检测荧光素酶报告基因的方法，观察用两种不同的方法处理细胞后对EDAG基因表达的影响。

K562 cells were induced by PMA or transfected with BCR-ABL chimeric gene , and the expression of EDAG gene in the reporter cells was determined with luciferase assay .

结论：建立的基于荧光素酶报告基因的体外雌激素样物质检测方法有效可靠，以此方法检测2,4-DDT和METH显示有明显的雌激素样活性作用。

Conclusion : The reporter gene-based assay we established is effective and reliable ; 2,4-DDT and METH demonstrate obvious estrogenic activities when detected by our method .

2型糖尿病患者LDL和VLDL使HUVEC的PAI1基因调控区（1528/+55）荧光素酶报告基因活性显著增高（P<0.01）。

LDL and VLDL from patients of type 2 DM enhanced the activation of PAI-1 ( - 1528 / + 55 ) luciferase reporter gene transiently transfected in HUVEC ( P < 0.01 ) .

运用KSHVRta基因启动子（KSHV复制时最先被激活的启动子）驱动的虫荧光素酶报告基因进一步证实并扩展研究结果。

The results were extended and confirmed using a luciferase reporter construct driven by the KSHV Rta ( replication and transcription activator ) promoter , the first promoter activated during KSHV replication .

方法经RT-PCR检测皮质酮处理的Leydig细胞中Egr-2及Egr-3的mRNA的水平，采用双荧光素酶报告基因系统评价Egr-2和Egr-3表达载体对Leydig细胞中FasL启动子活性的影响。

Methods The levels of Egr-2 and Egr-3 mRNA in Leydig cell subjected to CORT treatment were investigated by RT-PCR . The ability of Egr-2 and Egr-3 to activate the FasL promoter in CORT-treated Leydig cell was detected by Dual-Luciferase Reporter Assay System .

β1整合素启动子荧光素酶报告基因载体的构建与鉴定

Construction and identification of β _1 integrin promoter-luciferase reporter gene recombinant vectors

萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定

Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene

我们用荧光素酶报告基因的方法，探讨了这个问题。

We addressed this question with the method of luciferase reporter gene .

方法采用荧光素酶报告基因方法。

Methods The method of luciferase reporter gene was used .

为了支持这一观点，我们用双荧光素酶报告基因分析的方法进行验证。

To confirm this hypothesis , we used dual luciferase reporter gene assay .

虫荧光素酶报告基因用于二恶英类化学物质的检测

Detection of Dioxin-type Chemicals by Luciferase Reporter Gene

二恶英反应增强子调控的虫荧光素酶报告基因质粒的构建

Construction of Luciferase Reporter Plasmid Which is Under the Control of Dioxin - responsive Enhancers

方法采用电泳迁移改变分析和荧光素酶报告基因瞬时转染分析。

Methods Electrophoretic mobility shift assay and transient transfection assay were used in this study .

β-防御素-2荧光素酶报告基因载体及克罗恩突变体的构建

Construction of luciferase reporter gene plasmid containing hBD-2 and mutant correlated with Crohn 's disease

对发现的突变，进行荧光素酶报告基因瞬时表达研究。

The reporter gene was used to study the mutation influencing the activity of promoter .

利用Gal4/VP16-UAS和双荧光素酶报告基因系统检测γ-分泌酶活性

Applying Gal4 / VP16-UAS and Dual-Luciferase reporter gene system to detecting the activity of γ - secretase

第三，用荧光素酶报告基因，观察淫羊藿和枸杞的雌激素作用。

Third , we use Luciferase reporter gene to screen phytoestrogen from Herba Epimedii and Wolfberry fruit .