液相杂交

核酸扩增-液相杂交法检测沙眼衣原体DNA

Detection of the chlamydia trachomatis DNA by PCR - liquid hybridization method

乙型肝炎病毒聚合酶链反应液相杂交酶免疫检测的定性判断值研究

Study on the qualitative decision value in PCR-ELISA with liquid hybridization for detecting HBV

种特异性的探针以液相杂交的方式检测PCR产物。

The species specific probe detects PCR products with solution hybridization .

目的：探讨用核酸扩增（PCR）－液相杂交法检测沙眼衣原体DNA的效果。

Aim : To study the method of detecting chlamydia trachomatis ( CT ) DNA by PCR-liquid hybridization .

串联重复序列信号放大液相杂交检测HBV-DNA技术的建立及评价

Development and Evaluation of a Tandem Repeat Signal Enhanced-Liquid Hybridization Assay for Hepatitis B Virus DNA

结论：液相杂交是一种较NorthernBlot更为快速、简便和敏感的检测miR-28的方法。

It is concluded that the solution hybridization for detection of miR-28 in B cell lymphoma cell lines is a faster and more sensitive method as compared with standard Northern blot technique .

为了进一步明确miRNA的加工机制及功能和定量研究miRNA的表达水平，应用液相杂交和NorthernBlot两种方法检测miR-28在B细胞淋巴瘤细胞系中的表达并进行了比较。

To further understand the biogenesis and functions of miRNA , and to quantify miRNA expression levels , the expression of miR-28 in B lymphoma cell lines was detected by solution hybridization and Northern blot , and their detected results were compared .

方法：用PCR－液相杂交法对616份临床标本进行了沙眼衣原体检测，并与培养法比较，对与培养法结果不相符合的标本及部分阴性标本进行连接酶链反应（LCR）复检。

Meth-ods : A total of 616 urogenital specimens from sexually transmitted disease ( STD ) clinics were subjected to CT DNA detection by PCR-liquid hybridization method , and the result was compared with that of the cell culture method .

液相杂交技术在聚合酶链反应酶联免疫检测的应用

Liquid phase hybridization in polymerase chain reaction enzyme linked immunosorbent assay

微孔板液相杂交可同时检测多种深部真菌。

Our liquid phase hybridizationfor PCR-ELISA can detect multi-fungi at the same time .

目的：在建立新的微生物DNA液相定量杂交法的基础上，检测了10株临床分离菌间染色体DNA的杂交率并与固相板法进行比较，为该法的推广应用奠定了基础。

Objective : To use increasingly a new method of DNA-DNA hybridization in free solution which we established recently , we measured the genetic relatedness among 10 clinic strains of bacteria through this way and made a comparison with solid phase hybridization .

目标扩增区域及内参片段的上游引物采用生物素标记，以便于液相芯片杂交检测。

Target amplified region and the forward primer of internal reference fragments were labeled with biotin in order to facilitate hybridization detection of liquid chip .

结果表明：①液相PCR比原位杂交和间接原位PCR检测的灵敏度高：②间接原位PCR比原位杂交灵敏度高，检测到基因疫苗在被免疫BALB/c小鼠中存留时间更长并且检测到杂交信号更强；

Sensitive of ISPCR is higher than ISH and gene vaccine could be detected in longer time BALB / c mice , at the same time , intension is higher ;