肠激酶

经SDS-PAGE测定，纯化的重组肠激酶纯度达80%以上，并能高效切开融合蛋白。

The purified enterokinase reached a purity of above 80 % by SDS-PAGE analysis and showed high activity at fusion protein .

融合牛肠激酶轻链EKL包涵体蛋白的复性纯化

Refolding of the Fusion Protein of Recombinant Enterokinase Light Chain rEK_L

采用谷眺甘肽亲和层析柱纯化GST融合蛋白，经肠激酶特异性裂解释放出目的蛋白。

The GST fusion protein was purified by glutathione affinity chromatography .

牛肠激酶催化亚基cDNA的克隆、表达及表达产物的纯化和应用

Purification and Application of Bovine Enterokinase Catalytic Subunit ( EK_L ) Expressed from the Cloned cDNA Encoding EK_L

利用肠激酶酶切该重组噬菌体，获得了高滴度的重组噬菌体，证明了模拟表位N末端的噬菌体蛋白残余序列会妨碍其与OTA抗体的结合。

We obtain a high titer recombinant phage by using Enterokinase cleavage . It indicate that the residues in N-terminal of p ⅷ may prevent mimotope binding with antibody .

SDS-PAGE凝胶电泳发现，转基因油菜油体肠激酶酶切前后蛋白条带发生了明显改变，说明肠激酶可以对纯化的油体进行有效切割。

By SDS-PAGE , we found that the protein band of oil body changed significantly after digestion by enterokinase , indicated that enterokinase was effective to cleave purified oil body .

目的：制备能在原核表达系统中表达并特异性识别（Asp）4-Lys序列的鼠源性肠激酶，为推广应用重组肠激酶提供技术平台。

Objective To establish a prokaryotic expression system for expressing mouse enterokinase which could recognize and cut the sequence of ( Asp ) _4-Lys in order to provide a technique platform for application of recombinant enterokinase .

研究也发现，无论蜂毒肽与EGFP蛋白融合表达，还是进一步将表达的蜂毒肽利用设计的共表达肠激酶切割下来，其对Hela细胞都具有促凋亡的作用，促凋亡的效果没有明显的区别。

Further research showed that both melittin and its fusion protein EGFP-Mel had their role in apoptosis of Hela cells and no significant difference was observed in the effects of apoptosis .

肠激酶（Enterokinase，EK）是目前生物制药领域纯化重组蛋白产品时用于切割融合蛋白的首选工具酶之一。

Enterokinase ( EK ) is one of the most useful tools as a fusion protein cleavage regent for purification of recombinant protein production in biopharmaceutical industry .

经金属离子亲和吸附、肠激酶消化以游离vMIPⅡ、阳离子交换层析等步骤纯化目的蛋白。

Purified vMIP ⅱ was produced through metal chelated affinity chromatography , enterokinase digestion to free vMIP ⅱ from fusion protein and cation exchange chromatography .

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

Recombinant hKS with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification .

牛肠激酶轻链在毕赤酵母中的分泌表达、纯化和活性鉴定

Secretory Expression , Purification and Characterization of Bovine Enterokinase Light Chain

肠激酶特点及其基因工程的研究进展

Characterization of Enterokinase and Its Research Advances in Genetic Engineering

牛肠激酶轻链基因的克隆及其在大肠杆菌中的融合表达的研究

Clone and Expression of Bovine Recombinant Enterokinase Catalytic Subunit in Escherichia Coli

重组肠激酶在大肠杆菌中的发酵与纯化

Fermentation and Purification of Recombinant Enterokinase by Producing in E.coli

重组鼠源性肠激酶轻链在大肠杆菌中的表达

Expression of recombinant mouse enterokinase light chain in E.coli

重组大肠杆菌生产肠激酶的研究

The Study of Enterokinase Produced by Recombinant E.coli

牛肠激酶基因工程菌的构建、高密度发酵、纯化及活性鉴定

Construction , High-Density Fermentation and Purification of Recombinant Enterokinase Catalytic Subunit in Pichia pastoris

重组肠激酶在甲醇酵母中高效表达的培养条件研究

The influence factors for the high-level expression of recombinant enterokinase in the Pichia pastoris

利用肠激酶加工融合蛋白的方法制备重组人甲状旁腺素（1～34）肽

Enterokinase Cleavage of Fusion Proteins for Preparation of Recombinant Human Parathyroid Hormone 1 & 34

对重组肠激酶在大肠杆菌中的高密度发酵表达条件、纯化方法及其生物活性测定进行了研究。

The optional high cell-density fermentation conditions , purification methods and biological activity of the recombinant enterokinase in E.

结论：鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

Coli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized .

在酶切融合蛋白时，除正常切割和非特异性切割以外，发现了牛肠激酶的一个可能的次特异性识别序列。

Apart from normal specific cleavage and nonspecific cleavage , a possible sub-specific site was discovered in cleaving a fusion protein with enterokinase ;

研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。

Objective : To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins .

胰蛋白酶是以无活性的酶原形式存在于鱼类胰脏中，被肠激酶或有活性的胰蛋白酶自身激活，分子构象发生一定改变后转变为有活性的胰蛋白酶。

Trypsin exists in fish 's pancreas with inactive zymogen , which is activated by enterokinase or trypsin , and its molecular structure has changed from trypsinogen to trypsin .

经表达条件优化，大量制备重组蛋白，变性条件下进行镍离子亲和层析纯化。对纯化后的重组蛋白进行复性，用肠激酶切割复性蛋白以获得目的多肽。

The polypeptide of interest was purified by Ni2 + affinity chromatography under denaturing conditions , followed by renaturation of the recombinant protein by dialysis , and digestion with enterokinase to release the chimeric peptide .

该粗酶经脱盐后在适宜的缓冲体系中21℃温育过夜，显示出较高的自催化切割活性，为进一步进行重组牛肠激酶活性的研究及应用奠定了基础。

After desalting and changing buffer , the crude kinase was incubated at 21 ℃ overnight and demonstrated a high autocatalytic cleavage activity . This investigation would be able to lay a foundation for enterokinase activity research and farther application of expression products on a large scale .