缓冲液

样品处理采用固相萃取法，流动相为乙腈：磷酸盐缓冲液：水（15：5：85），检测波长：栀子苷240nm，芍药苷232nm。

The mobile phase for serum sample consisted of a mixture of acetonitrile : PBS : water ( 15 : 5 : 85 ) . The UV detector was set at 240 nm and 232 nm respectively .

缓冲液pH值对肽核酸压电基因传感器阵列杂交效应的影响

Effects of pH values of buffer on hybridization efficiency with PNA piezoelectric gene sensor array

结果表明，不同pH处理能够显著影响蒸腾速率，同一缓冲液不同pH值下的蒸腾速率差异较大；

The results showed that pH treatment could significantly influence the transpiration .

研究了载药纳米球在pH为7.4的磷酸盐缓冲液中的药物释放行为。

Drug release behavior was studied in the pH 7.4 phosphate buffered saline .

实验结果表明.在pH为4.50的HAc-NaAc缓冲液中，Vc在该电极上的线性范围为6.0×10~（？）

In pH 4 . 50 HAc - Na-Ac buffer solution .

不同pH值缓冲液对玉米叶片蒸腾速率和相对含水量的影响

Effects of different pH buffer on transpiration and relative water content of maize leaf

磷酸盐缓冲液pH值对利福平滴眼液吸收的影响

Influence of the pH Value of Phosphate Buffer on the Absorbency of Rifampicin in Eye Drops

考察了梯度洗脱分离时，起始缓冲液组成及pH值对分离的影响。

The effects of initial buffer composition and pH on the gradient elution have been investigated .

分生孢子萌发温度范围为10-25℃，最适为18-20℃，在pH2.2-8.34缓冲液中均能萌发，最适pH为6。

The temperature range of conidia germination is 10-25 ℃ with the optimum of 18-20 ℃ .

比较在不同pH的缓冲液溶剂中，新乌头碱体外渗透速率的差异。

To compare the percutaneous speed ( J ) of MA with different pH buffer solution .

卷烟烟雾磷酸缓冲液中H2O2生成量的测定

Determination of the Amount of Hydrogen Peroxide Generated from Cigarette Smoke in Phosphate Buffer Solution

方法：选用不同pH的正极缓冲液和样品蛋白上样量，应用非变性聚丙烯酰胺凝胶电泳分离大鼠海马组织蛋白质。

Methods : Nondeture PAGE was used to separate proteins in different pH and loading quantity of protein sample .

由于降解过程中释放出酸性的乳酸小分子，因此降解后缓冲液pH值显著降低。

Since lactic acid molecules were released during the degradation process , pH value of the buffer solution lowered significantly .

同时对温度，pH值和缓冲液组分浓度等影响因素作了系统的研究。

The effects of temperature , pH , ionic intensity and buffer concentration on the hydrolysis rate constants were discussed .

RB&SE的浓度、缓冲液pH、标记反应温度对标记效率有影响。

The labeling effect of RB-SE was affected by concentration , pH value of buffer solution and labeling reaction temperature .

不同pH值缓冲液处理下蚕豆叶片相对含水量、脯氨酸及丙二醛含量的变化

Change of relative water content , Proline content and Malondialdehyde content of Vicia faba leaves under different pH buffer treatments

洗衣粉和高pH缓冲液浸种对黄瓜子叶离体培养物花芽分化的影响

Effect of seeds pretreated with detergent and high pH buffer on the floral differentiation in cucumber cotyledons cultured in vitro

利用微量过饱和静置法，在柠檬酸缓冲液中培养出可供X射线结构分析用的去B链N端二肽（B（1-2））猪胰岛素单晶。

Des-B1-2 Pig insulin single crystals suitable for X-ray structural analy-is have been grown by the still-setting method in a buffer containing citrate .

静态吸附法主要考察了缓冲液pH值、缓冲液钠离子强度、吸附温度和吸附时间等对木瓜蛋白酶吸附量的影响。

The static adsorption mainly concerned on the buffer pH , Na ionic strength , the adsorption temperature and adsorption time .

方法用不同缓冲液制备斯氏狸殖吸虫成虫粗制抗原，并用聚丙烯酰胺凝胶电泳（SDS－PAGE）方法对其蛋白组分进行分析。

Methods Using SDS - PAGE method to analyse soluble protein antigens and two buffer preparate antigens .

在Tris-HCl/ProteinaseK缓冲液中降解10小时后，电纺膜减重率达到了39.09%。

Weight loss of electrospun membrane was 39.09 % after 10 hours of degradation in Tris-HCl / Proteinase K buffer solution .

用含Ca~（2+）缓冲液清洗层析柱后（洗脱物Pc为另一重要产品），该柱可反复使用。

The affinity chromatographic column could be washed with a buffer containing Ca2 + and the PC eluted product could be reused .

此外，还讨论了不同缓冲液浓度、十二烷基硫酸钠（SDS）浓度及甲醇含量对2种有效成分迁移行为的影响。

The effect of concentration of buffer , SDS and methanol were also discussed .

笔者还讨论了表面活性剂SDS的浓度、缓冲液的pH值及运行电压对黄酮类有效成分迁移行为的影响。

The effects of pH. surfactant concentration and applied voltage on the migration behavior of the solutes were studied .

同时比较固定相、流动相、缓冲液、pH值、洗脱速度和上样体积对分离结果的影响。

The separation conditions including stationary phase , mobile phase , buffer , pH value , eluotropic rate , and loading capacity were analyzed .

同时考察温度、缓冲液浓度及pH值等因素对动态吸附量、突破曲线形态等色谱性能的影响；

Effects of some variables such as temperature , buffer concentration and pH on the binding capacity and breakthrough curves of the columns were examined .

方法以蛋白质标准品、临床尿液标本为实验对象，通过提高缓冲液的pH值、在缓冲液中加入适当的添加剂等方法，进行芯片蛋白电泳，并与常规琼脂糖电泳结果进行比较。

Methods The standard model proteins and clinical urine specimens were analyzed by improvement of pH and introduction of additive and compared with gelose electrophoresis .

cDNA的合成首先在逆转录缓冲液中进行，然后取出1/10的反应产物进行PCR。

CDNA synthesis in the first reverse transcriptase buffer , and then remove 1 / 10 of the reaction products for PCR .

结果表明：适合苹果花粉处理的缓冲液蔗糖浓度为10%，pH值为54；

The results showed that the suitable buffer pH value fit for apple pollen treatment was 5.4 and the suitable concentration of cane sugar 10 % .

擦干切片，温育，PBS缓冲液浸泡冲洗。

Dry the slice , temperature nourishing , and PBS buffer for irrigation .