标记探针

地高辛标记探针检测重组双功能水蛭素中的DNA残留量

Analysis for Residual DNA in Recombinant Double Functions Hirudin Using Digoxin Labeled Probe

地高辛标记探针检测生物制品中残余DNA含量

The detection of the residual DNA in the biological products by labeled probe of digoxigenin

用缺口翻译法制备a-~（32）P标记探针。

The a - ~ ( 32 ) p-labeled DNA probe was prepared by nick translation .

地高辛随机引物法标记探针的Southern杂交技术优化

Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method

双峰驼线粒体DNA地高辛标记探针的制备及其应用

Preparation of mtDNA Probe Labeled with Digoxigenin and Its Application to mtDNA RFLP of Two humped Camel

PCR和随机引物标记探针的方法比较

The comparison between PCR and random primer labeled method

地高辛素标记探针原位杂交法检测肝和肝外组织中乙型肝炎病毒DNA

Preparation of digoxigenin labelled probe and detection of HBV DNA in liver and extrahepatic tissue with in situ hybridization

应用生物素标记探针原位检测星形细胞瘤组织中p53基因

Detection of p53 gene by isotopic probe in astrocytoma tissue

无标记探针技术检测IL-6基因启动子单核苷酸多态性

Unlabeled probe genotyping for detection of single nucleotide polymorphism of IL-6 promoter

PCR标记探针原位检测培养的海马神经细胞BDNFmRNA的表达

In situ detection of BDNF mRNA in cultured hippocampal neurons with probe labeled by PCR

应用Digoxigenin标记探针定量检测血清HBVDNA

Quantitation of HBV DNA in Serum by Dot Hybridization Applying Probe Labelled with Digoxigenin

目的用地高辛标记探针的方法进行Southern杂交，以检测转人组织蛋白酶K基因小鼠外源DNA的整合情况，筛选携带目的基因的阳性小鼠。

Objective Southern blot hybridization with DIG labeling probe was used to identify whether transgenic mice of human Cathepsin-K gene were positive .

目的建立用非同位素标记探针杂交测定端粒DNA长度的方法，并探讨其法医学的应用价值。

Objective To measure telomere length by hybridization with non - radio - isotopic - labeled probes and study its application in forensic medicine .

本文对免疫PCR的方法、DNA标记探针的构建及扩增产物的检测进行了综述。

This review summaries the methods of immuno PCR , the construction of DNA label probe and the detection of amplified products .

标记探针能与克隆DNA、恶性疟原虫Fcc1/HN株和云南株的PCR扩增产物杂交。

The labeled probe can hybridized with the PCR amplified products of cloned DNA , P.f Fcc1 / HN strain and Yunnan strain .

结论该方法可用于猪的DNA指纹分析，其效果与同位素标记探针的效果基本一致。

Conclusion This method can be used to DNA fingerprinting of pigs and the same effect can be achieved at that generated from the isotopically labelled probe .

非同位素标记探针杂交分析白细胞端粒DNA长度

Telomere length measurement of human leukocyte in peripheral blood by non-radio - isotopic hybridization ; Study on the Aggregation of Lable-free Nucleic Acid Probe Induced by DNA

应用生物素标记探针对癌周肝硬变组织中HBV-DNA的原位分子杂交研究

Identification of HBV-DNA in cirrhotic liver tissue by in situ hybridization with biotin-labeled probes

方法：以33p-dCTP标记探针杂交筛选人骨骼肌cDNA文库，对阳性克隆进行序列分析，并以脂质体介导转染哺乳动物细胞，用免疫印迹及间接免疫荧光染色法检测目的蛋白的表达。

METHODS : 32p-dCTP-labeled probe hybridization was used to screen the human skeleton cDNA library and sequence of the positive clones were analyzed .

地高辛标记探针原位杂交检测肝内HDVRNA

Detection of Hepatitis D Virus RNA in Liver Tissue by in situ Hybridization with Digoxigenin-labeled HDV cDNA probes

PCR法制备地高辛标记探针原位杂交在人结核杆菌Edman株基因检测中的应用

Detection of Mycobacterium Tuberculosis Edman Strain by Situ Hybridization with Digoxigenin Labeled Probe by PCR

方法：1、根据HCVe1基因序列，设计并合成引物和荧光标记探针。

According to the specific sequence of HCV E1 gene , the primers and the fluorescence probe were synthesized as designed .

应用同位素标记探针的原位杂交试验及PCR技术均未检查出类志贺样毒素Ⅰ和Ⅱ（SLT－Ⅰ，SLT－Ⅱ）。

Shiga-like toxin and were not detected with in situ hybridization of radio isotope labeled probe and PCR .

地高辛标记探针检测Vero细胞狂犬病疫苗残余Vero细胞DNA含量

Detection of residual Vero cell DNA contents in purified Vero cell rabies vaccine by a dot blot hybridization assay using a digoxigenin labelled probe

目的：利用非放射性标记探针技术检测经聚合酶链反应扩增后的人粪便中微小隐孢子虫DNA，观察该方法的特异性和敏感性。

AIM : To develop a non radioactive labeled probe hybridization technique for the detection of the PCR products of Cryptosporidium parvum DNA in fecal samples and evaluate its specificity and sensitivity . METHODS : DNA of C.

用国产光生物素标记探针，经RNA-DNA斑点杂交技术检测了41例白血病患者中c-myc和c-fos原癌基因的RNA表达水平。

The c-myc and c-fos proto-oncogenes were detected in forty-one leukemia patients by RNA-DNA dot blot hybridization with photobiotin probes .

首先进行芯片杂交动力学研究，考察信号值和Ratio值与PCR产物点样浓度、PCR产物长度和荧光标记探针长度之间的关系，探讨这三者对SNP分型的影响；

We first studied the hybridization dynamics to optimize the conditions of this method by observing how spotting concentration of PCR products , PCR products length and fluorescence labeled probe length affect genotyping ;

应用PCR法制备地高辛标记探针原位杂交检测绵羊卵巢褪黑素受体Mel1a基因表达

Detection of express Mella gene in ovary of ewe by In situ hybridization with Digoxigenin labeled probe by PCR

用聚合酶链反应（PCR）和地高辛标记探针（dig－probe）检测临床血沉标本中人巨细胞病毒（HCMV）DNA。结果表明，人群血标本中HCMV－DNA携带率较高；

Human cytomegalovirus ( HCMV ) DNA in 78 clinical blood specimens of determining erythrocyte sedimentation rate ( ESR ) wasdetected by polymerase chain reaction ( PCR ) and digoxigenin labelled probe ( dig-probe ) .

方法用地高辛标记探针的方法，对转人组织蛋白酶K基因小鼠（F0代）及首建阳性鼠与野生型ICR小鼠交配产生的F1代进行Southern杂交，对F0代和F1代进行筛选。

Methods Transgenic mice and the intercrossed progeny between the founder mice of human Cathepsin-K gene and wild ICR were detected by Southern blot hybridization with DIG labeling probe .