细胞破壁

以可溶性蛋白为参照，通过蛋白质的SDS-PAGE分析，比较4种处理方法使细胞破壁后释放蛋白质效果的差异。

With releasing soluble protein as example , the differences of releasing proteins by the four methods of breaking cell walls of fungus were analyzed with SDS-PAGE .

碱液（KOH溶液）浓度为5.5%时最有利于放线菌细胞破壁，破壁时间为30分钟。

Lye ( KOH solution ) a concentration of 5.5 % is most beneficial actinomycetes cells , broken , broken time is 30 minutes .

方法金银花、黄芪、赤芍经真空气流植物细胞破壁技术处理后，热水浸泡1h作为新技术组；

METHODS The new technology group : Flos Lonicerae , Radix astragal and Radix Paeoniae rubra with the vacuum air current for plant broken cell wall , and then soaked 1 hour with hot water , The traditional technology group : draw above each medicine according to tradition drawin technology .

酵母细胞破壁技术研究与应用进展

Study and applications of technology about breaking yeast cell wall

真菌细胞破壁方法的研究

Study on the Methods of Breaking Wall of Fungus Cell

结论超微粉碎可使桑叶细胞破壁。

ConclusionThe walls of the cells were broken .

真空气流植物细胞破壁技术在中药领域的应用

Application of the Vacuum Air Current for Plant Cell Wall Broken in Chinese Medicine

论文针对目前提取花色甙色素过程中温度较高、提取效率低等不足，将超声波法和冻结-融解法这两种生物技术中常用的细胞破壁方法应用于花色甙色素的提取。

Aiming at increasing extraction efficiency , ultrasonic method and freezed-melt method were used in extracting anthocyanins pigment from purple sweet potatoes .

该工艺包括雨生红球藻细胞破壁，虾青素的提取，虾青素酯的水解和游离虾青素的纯化分离。

The process included the cell disruption of Haematococcus pluvialis , the extraction of crude astaxanthin , the hydrolysis of astaxanthin esters and the purification of free astaxanthin .

采用超微粉碎技术，细胞破壁，可以大大提高有效成分的释放速度和吸收，增强药效，降低服用剂量。

The superfine grinding technology , the cell , the wall , can greatly enhance the effective component release rate and absorption , enhance the efficacy , lower dose .

通过碘化钾分解、酵母微生物细胞破壁以及油脂和纤维素水解实验结果，说明了水力空化对化工过程的强化作用；

The enhancement effects of hydrodynamic cavitation on chemical processes was demonstrated by experimental results of KI decomposition , disruption of yeast cells , and hydrolysis of fatty oils and cellulose .

目的考察真空气流植物细胞破壁技术与传统提取工艺的质量对比。

OBJECTIVE To compare the quality difference of Flos Lonicerae , Radix astragal and Radix Paeoniae rubra with the vacuum air current for plant cell wall broken and the traditional drawing technology .

但目前我国没有专门用于污泥破解的超声波反应器，实验室用的微生物细胞破壁超声波反应器或超声清洗器破解污泥时，最大缺陷是能耗高。

But , at present , professional ultrasonic reactor in the field of disintegration of waste activated sludge is not found in China . Ultrasonic reactor of microbial cell lysing or ultrasonic cleaner is used in laboratory , and it is low efficient and high energy-consuming .

研究中还找到一个简便有效的对β-胡萝卜素的提取方法，选用盐酸-热处理法进行细胞破壁，并选用沸程为60～90℃的石油醚进行萃取。

In the extracting study , it has showed hydrochloric acid-heat treatment is a simple , convenient and effective extracting methods is which was used to destroy the cell wall , and the extracting organic solvent is petroleum ether whose boiling range is 60 ~ 90 ℃ .

本论文对子囊菌DS-9701菌体细胞进行了破壁方法的选择研究，并采用SDS-PAGE分析方法研究了子囊菌DS-9701在不同培养温度下生长，其细胞可溶性蛋白质表达的差异性。

We carried out the optional research of the method that be acceptable for breaking up the cell wall of Ascomycetes DS-9701 . and used analysis of SDS-PAGE to research expressional distinction of soluble protein of cell during Ascomycetes DS-9701 was cultivated under different temperatures .

对酵母细胞酶法破壁的研究

Research on yeast cell breaking by enzyme

方法：用红、白细胞裂解液、破壁酶和真菌DNA提取盒直接处理血标本，得到的微量靶DNA用白色念珠菌种特异性引物进行扩增。

Methods : The blood specimens were directly disposed with erythrocyte lysis buffer , leukocyte lysis buffer and fungi DNA extract kit , and then a PCR was carried out with Candid albicans species specific primers .

结果表明，在微波和传统溶剂加热的作用下，植物细胞的结构都会发生较为明显的变化，主要表现在质壁分离现象，叶绿体等胞内物质被破坏，但微波辅助萃取可使细胞破壁。

The result showed that the cell structure changed when the leaves were under microwave radiation or solvent heating , and the cytoderm was broken in microwave-assisted extraction , while it was not broken in traditional solvent heating .