溶壁酶

本文还就CaCl2，MgCl2及低温保藏（3&4℃，30天）对于原生质体稳定性与再生的影响作了初步探讨，并证实溶壁酶对原生质体有明显的破坏作用。

The effects of CaCl_2 , MgCl_2 and low temperature storage ( 3 & 4 ℃ for 30 days ) on the stability and regeneration of the protoplasts were also discussed , and the protoplast damage caused by Lywallzyme was detected .

应用冷冻干燥技术，可使溶壁酶破壁灵芝孢子粉的有效成分（多糖）的保护率在99%左右。

And the protection rate of effective component could reach 99 % with lywallzyme sporoderm-broken plus freeze-drying technique .

真菌溶壁酶的最适消化浓度是6.0mg/ml，作用时间是1.5小时；

The best enzymes was fungus cell wall degradation enzyme and the optimal concentration , treatment time were 6.0 mg / ml and 1.5 hours .

真菌细胞溶壁酶的产酶培养和应用效果

Production and Application of Fungal Cell Wall Degrading Enzyme

溶壁酶的破壁效果最好；

Ly wall zyme is the best enzyme in breaking the cell wall of the hyphae ;

溶壁酶通过水解细菌细胞壁肽聚糖上糖与肽间的酰胺键或肽内氨基酸残基间的连键，从而使细菌裂解。

Lysins mostly hydrolyze covalent bonds between the sugar and peptide or peptides essential for peptidoglycan integrity , causing bacterial lysis .

通过对溶壁酶组成及浓度，渗透压稳定剂、菌丝培养方法等研究，建立了有效的双孢蘑菇原生质体制备与再生系统。

Based on testing of different combinations and concentration of lysozymes , osmotic stabilizers and different methods of mycelium incubation , an efficient system of protoplast preparation and regeneration in Agaricus bisporus was established in present paper .