目的基因

Northern点杂交结果表明目的基因在多数转基因水稻植株体内已进行了转录；

Northern blot hybridization showed the target gene has been transcripted into mRNA .

结果：通过重叠PCR获得序列正确的目的基因。

RESULTS : The target gene with correct sequence was obtained by overlapping PCR .

对重组质粒DNA进行纯化，并对目的基因片段进行核苷酸序列分析。

The recombinant plasmid was purified and the target DNA fragment was sequenced .

一种优化的PCR方法&降落PCR扩增目的基因

TD PCR & an ameliorative PCR for amplification of target genes

经PCR检测，证明目的基因已整合到烟草基因组中。

PCR detection demonstrated that the fusion gene has been integrated into tobacco genome .

获取理想的目的基因PCR反应的综合参数比十分重要。

Synthetic parameters of the PCR are very important to obtain ideal target gene .

以非复制型HIV为载体进行目的基因转移的实验研究

Study on High-efficiency Gene Transfer of Pseudotyped HIV Vector

果树核DNA提取、目的基因分离与克隆技术研究进展

Advances in the techniques for extraction of nuclear DNA and isolation and cloning of target genes in fruit trees

结论：逆转录PCR技术是获得目的基因的有效方法。

Conclusion : Reversible transcription PCR technique is an effective method for obtaining definite gene .

实验中还对目的基因之后的Nos终止序列区进行了扩增，通过NosTer。

Nos terminating sequence which was behind thymosin gene was amplified .

RT-PCR检测表明目的基因在RNA水平上的表达。

RT-PCR was used to demonstrate the expression of the target genes in RNA level .

猪囊尾蚴cDNA表达文库的构建、筛选及目的基因的克隆和鉴定

The Construction Screening of cDNA Expression Library of Cysticercus Cellulosae and Cloning Identification of Interested Genes

，经PCR扩增和Southernblot分析，证明外源双价目的基因已同时整合到转基因植株的基因组中。

PCR and Southern blot demonstrated that the two genes were integrated into the genome of the tobacco plants .

对T1代转基因生菜植株进行的PCR检测结果表明，目的基因能够遗传，但遗传率很低。

But the rate of genetic of lysine rich protein gene in the lettuce was low .

测序结果显示所购克隆为目的基因克隆，电泳结果显示提取的质粒和PCR产物保持了较好的纯度和均一性。

Electrophoreses results revealed good quality of extracted plasmids and PCR products . Sequencing results showed that the genes were the correct ones .

RNAi的机制是通过一段小片段双链RNA的作用序列特异性地使一段目的基因失活。

RNAi silences gene in a sequence-specific manner through the actions of small fragment of double-stranded RNAs .

初步显示再生黄芩植株携带有目的基因，尚需进行Bt基因的Southernblot证实。

It preliminary shows that regeneration plants carry the foreign genes , while Southern blot analysis is needed to confirm it .

SDS-PAGE结果发现，目的基因以包涵体的形式表达；

The SDS - PAGE detection demonstrated that the 6S gene expressed the protein with inclusion body .

GUS染色证明外源目的基因在玉米细胞和组织中得到了表达。

GUS assay indicated that foreign gene had expressed in maize cell and calli .

这种方法需要通过PCR扩增获取目的基因的一段序列或通过测定植酸酶蛋白的部分氨基酸序列来设计杂交探针，也有通过表达来筛选基因文库的报道。

A hybridization probe is usually designed either by amplifying a fragment of the target gene or by determining partial amino acid sequence of the protein .

采用RealtimePCR的方法检测目的基因mRNA的表达情况，进而判断不同靶点的干扰效果。

Realtime PCR was performed to detect the expression levels of target gene mRNA , and then judge the interference effects of different target .

特异性试验结果表明：各单重PCR方法仅对目的基因有扩增，对其他病原或健康组织无扩增。

The specificity test results showed that only target gene was amplified by each single PCR method , while no amplification of other pathogens or healthy tissue .

DNA序列测定证明扩增产物中目的基因片段均为mage-acDNA序列。

The DNA sequencing confirmed that the target gene fragments in 2 samples of PCR products were mage-a cDNA .

本研究以抗病基因GO和抗病毒基因PAP为目的基因，研究了农杆菌介导法和基因枪法转基因定向改良嘎拉苹果抗病性。

The objective of the present study was to transfer the resistance gene GO and gene PAP to Gala apple by using particle gun and Agrobacterium-mediated methods .

结果显示，诱导后染色体DNA实现了高效重组，非目的基因片段消失，目的基因GUS在诱导后表达正常。

Results showed that the high-efficiency DNA recombination had take place and the target gene was working order after DNA excitation . Objective : ⅰ .

目的基因的PCR扩增，并将所得PCR反应产物通过琼脂糖凝胶电泳检测，用以进行氨基酸序列和及其生物信息学的分析。

PCR amplification of the target gene and PCR reaction products obtained by agarose gel electrophoresis detection , and for the conduct of amino acid sequence and bioinformatics analysis .

用发状根基因组RNA进行Northern-blot检测，也检测到了目的基因片段印迹，证明fps基因发状根基因组转录水平得到表达。

Northern blot analysis with RNA of hairy roots , detected objective gene fragment print ;

将pDisplay-mVP1转染PK15细胞，经3次G418加压筛选，并用RT-PCR和IFA鉴定，证明获得表达目的基因的PK15/pDisplay-mVP1细胞。

PK-15 cells expressing target gene were selected with G418 and identified by RT-PCR and IFA .

为建立以载体分子为基础的DNA计算系统，根据目的基因和载体分子连接操作的特性构造一类DNA计算模型。

A DNA computing model is established which simulates the mechanism of the ligation between target gene and vector molecular . The main idea is to study the DNA computing system which is based on vector molecular .

潮霉素抗性植株的PCR检测和目的基因的PCR检测表明，阳性率达到90%以上，初步确定目的基因已经整合到水稻基因组中。

PCR analysis of Hygromycin resistance gene and target gene showed that the positive rate was100 % , and preliminarily determined that the target gene had been integrated into the rice genome . 3 .