电泳缓冲液

实验还采用胶束电动毛细管色谱（MECC）对水溶性的蛋白质混合物进行了分析，电泳缓冲液中加入SDS后，水溶性蛋白质的吸附现象变的相对严重，分析效果有所下降。

And the separation of water-soluble proteins was done with micelle electro kinetic capillary chromatography ( MECC ) .

CZE的本质特点是整个毛细管内充盈的电泳缓冲液为均质且电场强度相同，待测物根据荷质比不同而被分离；

Fundamental to CZE are homogeneity of the buffer solution and constant field strength throughout the length of the capillary .

本论文系统研究了糊精类型、糊精浓度、电泳缓冲液浓度、电泳温度、进样时间等因素对毛细管电泳拆分苯乙醇胺的影响，建立了两种操作简单的苯乙醇胺检测方法。

Various factors affecting chiral resolution of phenylethanolamine , such as cyclodextrin type , concentration , buffer pH , and temperature were investigated , and two simple methods for determination of chiral phenylethanolamine were established by capillary zone electrophoresis .

方法：比较以往报道的各种低分子量蛋白的电泳分离方法，对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。

Methods : Based on several reported SDS-PAGE systems for low molecular weight protein , the buffer systems , concentrations of separating gel and stacking gel , electrophoresis procedures were optimized .

电泳采用中性毛细管涂层柱和4%线性聚丙烯酰胺凝胶电泳缓冲液，紫外检测仪检测。

The neutral coated capillary and 4 % linear polyacrylamide gel buffer were used , and the wave length of ultraviolet detector was 254 nm .