冷冻精子

另外，冷冻精子需要用外科用受精设备，严格采用内窥镜检查法（TCI，子宫颈受精法）将之注入母犬子宫内。

Also , with frozen , semen should always be put into the uterus using surgical insemination or insemination by rigid endoscopy ( TCI , Trans Cervical Insemination ) .

目的研究复温温度和白细胞介素1（IL1）对冷冻精子复温后存活率和顶体反应率的影响。

Objective To investigate the effect of thawing temperature and interleukin 1 ( IL 1 ) on human sperm by measuring viability and acrosome reaction rate .

本文报道了用咖啡因与肝素、钙离子载体（I-A）协同处理诱导马、驴、猪冷冻精子体外获能的研究结果.咖啡因与肝素、I-A均有协同作用。

The in vitro capacitation of frozen-thawed spermatozoa of stallion , jackass and boar induced by caffeine in combination with heparin or ionophore A23187 ( I-A ) was conducted in the study .

青鱼冷冻精子冰冻蚀刻的电镜观察

Electron Microscopic Observation on Freeze-Etching Samples of Freezing Sperm of Mylopharyngodon piceus

经皮睾丸微穿刺活检后冷冻精子的卵胞质内单精子注射

Intracytoplasmic Sperm Injection Using Cryopreserved-thawed Testicular Spermatozoa with Testicular Fine Needle Aspiration

冷冻精子受精能力的综合评价

Comprehensive evaluation for the fertilizability of frozen sperm

人类冷冻精子经卵黄缓冲液冷处理后的受精率变化

Change on Fertilizing Rate of Cryopreserved Human Sperm after the Cold Treatment of Egg Yolk Buffer

大部分冷冻精子结构完整，进行人工授精可以获得较高的受精率（71.5±11.6）%。

The quite high fertilization rate ( 71.5 ± 11.6 ) % was obtained with cryopreserved semen .

大西洋牙鲆冷冻精子×褐牙鲆卵杂交胚胎的发育及胚后发育

Embryonic and postembryonic development of hybrid produced with frozen sperms of Paralichthys dentatus and eggs of Paralichthys olivaceus

比提与妻子从一个冷冻精子银行买回精子，并在家接受了“人工受精”。

He and his wife bought donor vials from a cryogenic sperm bank and he was inseminated at home .

在自精保存工作中，如果精液质量较低，应将冷冻精子可能造成损伤的风险充分告知自精保存者。

In working , if semen quality is low , the patients should be informed of the risk of cryopreservation .

对36头西门塔尔、23头夏洛来和12头利木赞牛冷冻精子的实验结果表明：三个品种的牛冻精均能穿入去透明带仓鼠卵，并形成发育良好的雄原核。

We detected the frozen-thawed sperm of 36 Simmental , 23 Charolais and 12 Limousin , the results showed that the frozen-thawed sperm of these three breeds could penetrate the zona-free ova of hamster , and formed male pronucleuses .

结果冷冻后精子顶体酶活性明显低于冷冻前（P005）。

Results The sperm acrosin activities were significantly decreased after freezing ( P0.05 ) .

目的：对经皮睾丸微穿刺活检后冷冻保存精子卵胞质内单精子注射（ICSI）治疗非梗阻性无精子症所致不育进行临床总结，并对其影响治疗结果的因素进行探讨。

Objective : To evaluate the effect of intracytoplasmic sperm injection ( ICSI ) using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration ( TEFNA ) in patients with non-obstructive azoospermia .

MariamKukunashvili在接受来自墨西哥的电话采访时说，他还要求提供设备冷冻他的精子，这样他老的时候才会有足够的精子供应。

He also inquired about equipment to freeze his sperm tohave sufficient supply when he 's older , she said in a telephone interview from Mexico .

通过不同染色浓度和时间的比较发现，最适的AO染色浓度为200ug/mL，染色时间为5min。在此基础上，用AO染色法检测评价超低温冷冻前后精子DNA的完整性。

Comparing the different staining time and dye concentration , we found that final staining concentration of AO was 200ug / mL and the optimum staining time was 5 min. Then , we evaluate the effect of freezing-thawing process on sperm DNA integrity using AO staining .

几种因素对鸵鸟冷冻精液精子活力影响的研究

Influence of Several Factors on Sperm Vitality of Ostrich Freezing Semen

冷冻保存精子细胞所需要的条件较简单，容易成功。

Cryopreservation of spermatid is easy with simple conditions .

人类精液冷冻前后精子运动变化的计算机辅助分析

Comparison of Sperm Motion Parameters in Pre-freeze and Post-thaw Semen Samples Using Computer-Assisted Sperm Analysis

超低温冷冻保存精子为长期保存遗传资源提供了可能，但超低温冷冻会影响精子的生理和形态。

Spermatozoa cryopreservation offers potential for long-term storage of genetic resources . Many aspects of spermatozoa physiology and morphology are affected by cryopreservation .

结果新鲜精子的受精率（62.4%）略高于冷冻复苏精子的受精率（58.1%）；

Results : The fertilization rate of fresh sperm was higher than that of post-thaw sperm ( 62.4 % vs. 58.1 % ), but the difference was not significant .

冷冻以后精子的受精能力是保障哺乳动物高妊娠率的重要因素，与冷冻-解冻后精子质量密切相关。

The ability of fertilizing after cryopreservation is an important factor of high-pregnancy rates in mammalian after insemination , and it being associated with the quality of sperm after freeze-thaw process .

本研究对比了三头种公牛精子的分离效果；评估分离和冷冻对精子活力，体外受精效果以及胚胎发育的影响。

This study compared the sex-sorting effect of three bulls ; evaluated the influence of sex-sorting and cryopreservation on sperm viability and the effect of fertilization and the development capability of embryos .

乙二醇冷冻保存精子的质膜完整率最大（55.6±4.8%），乙二醇和甘油保护作用不显著（P>0.05）；

The rate of plasma membrane integrity with Dimethylsulphoxide reaches the highest ( 55.6 ± 4.8 % ) , the difference of Glycerol and Dimethylsulphoxide is not significant ( P > 0 . 05 ) .

在本研究中，我们主要观察：1.冷冻储存对精子的运动能力、畸形率、膜脂类过氧化反应、DNA损伤及线粒体膜电位、凋亡等的影响及各项指标之间是否存在相关性。

In this study , our main aim : 1 . The effect of cryopreservation on sperm motile ability , sperm morphology , membrane lipid peroxidation , DNA damage , mitochondrial membrane potential and apoptosis . 2 .

5ml大管低温冷冻对猪精子顶体和质膜超微结构的影响间质性子宫内膜异位症

Effects of 5 ml Straws Cryopreservation on the Quality and Ultrastructure of Boar Semen

肌酸磷酸对冷冻复苏后精子的活率和存活率的影响

Effect of Creatine Phosphate on Sperm Motility and Viability After being defrosted

目的：观察不同冷冻期冷冻精子的受精能力。

Objectives : To observe fertilizability of frozen sperms after different frozen times .

冷冻对人精子结构及功能的影响

Effects of freezing on human spermatozoal function and structure

冷冻对人精子遗传物质的影响

Effects of Cryopreservation on Human Sperm Genetic Substance