激光扫描共聚焦显微镜

本文运用激光扫描共聚焦显微镜和荧光探针技术，检测分裂细胞内DNA、RNA分布和含量的变化。

Morphology of the cell division and its content of DNA and RNA were measured under the laser scanning confocal microscope .

测定血清超氧化物岐化酶（SOD）、血清丙二醛（MDA）；细胞膜脂流动性检测采用激光扫描共聚焦显微镜。

The activity of superoxide dismutase ( SOD ) in serum and the yield of malondialdehyde ( MDA ) were measured with kit .

结论在采集快速变化的荧光图像时，CCD荧光显微镜系统优于激光扫描共聚焦显微镜系统。

Conclusions CCD fluorescent microscope is superior to laser scanning confocal microscope in collecting the quickly changing fluores - cent image such as DCF .

经染色体加倍处理所获得的2n花粉粒，体积明显比正常的花粉粒大，并将激光扫描共聚焦显微镜用于大花粉粒的倍性鉴定。

The volume of large pollen obtained from the experiment is larger than the normal one .

借助药理学试验和激光扫描共聚焦显微镜技术，进一步对该间接效应过程中是否有NO和H2O2的参与进行了探讨。

Whether NO and H_2O_2 were concerned with the adjustment in indirect ways was further explored by pharmaceutical experiment and laser-scanning confocal microscopy .

结果在激光扫描共聚焦显微镜下，可以清晰地观察Cx43和细胞膜。

Results The distinct images of Cx43 and cell membrane of detrusor muscle could be obtained by the LSCM .

取垂体，应用免疫荧光组织化学法结合激光扫描共聚焦显微镜（LSCM）分析技术，检测FS细胞中S-100表达。

The expression of S-100 in adenohypophyseal glands was detected by immunofluorescent histochemistry using laser scanning confocal microscopy ( LSCM ) .

而且，各种对虾卵子内皮质棒及皮层颗粒的形态结构、活化过程各有不同。本文就中国对虾卵子的活化及卵裂过程进行了激光扫描共聚焦显微镜（LSCM）研究。

The oocyte activation process and cleavage of Penaeus chinensis were studied using laser scanning confocal microscope ( LSCM ) .

Fluo-3负载激光扫描共聚焦显微镜观察细胞内钙离子水平。

The intracellular calcium level was observed by laser scanning confocal microscopy markered Fluo-3 / AM .

因此，在没有激光扫描共聚焦显微镜时，可用AdobePhotoshop7.0或imageJ图像处理软件将普通荧光显微镜采集的同视野两种单标记免疫荧光图像合成为保持原有特点、简便而清晰的双标记荧光图像。

Thus , in the absence of a confocal microscope , co-field double-stained immunofluorescence images can be obtained from single-stained images collected with a common fluorescence microscope using Adobe Photoshop 7.0 or Image J image-processing techniques .

目的：采用免疫荧光及激光扫描共聚焦显微镜技术，检测热水灌胃导致大鼠萎缩性胃炎后胃黏膜组织细胞Bcl-2及cox-2蛋白的表达。

AIM : To measure the expressions of Bcl-2 protein and cox-2 protein in gastric mucosa histiocyte of atrophic gastritis caused by high-hot water in rats with immunofluorescence and laser scanning confocal microscopy .

结论利用激光扫描共聚焦显微镜既可用定量测定又可动态观察培养活神经细胞内LPO含量变化。

Conclusion The results suggested that laser scanning confocal microscopy ACAS 570 may be used to measure and observe LPO concentration and its kinetic change in cultured living neurons .

利用免疫荧光技术和激光扫描共聚焦显微镜（共焦镜）技术检测了地塞米松介导小鼠腹腔巨噬细胞凋亡时凋亡抑制基因Bcl－2表达的时空变化。

By combined using of the immunofluorescence and laser scanning confocal microscope , the space-time changes in expression of apoptosis-inhibited gene Bcl-2 in apoptotic macrophages induced by dexamethasone were examined .

方法：（1）实验系统的建立：分别利用激光扫描共聚焦显微镜和CCD荧光显微镜采集ECV304细胞的DCF荧光图像，并比较两者的成像质量，实验可操作性等技术指标；

Methods ( 1 ) The device for study was determined by comparing the fluorescent images obtained by laser scanning confocal microscope and CCD fluorescent microscope respectively .

计算各组细胞死亡率、细胞生长抑制率、克隆形成率并观察形态学变化，应用凝胶电泳、激光扫描共聚焦显微镜观察分析小叶黑柴胡作用MGC-803细胞后的细胞DNA含量的改变。

The changes of morphology 、 death rate 、 the inhibition rate of cell growth 、 cloning efficiency were observed . DNA content of MGC-803 were measured with the laser scanning confocal microtechnic .

激光扫描共聚焦显微镜（LSCM）有效地排除了非焦平面信息，提高了分辨率及对比度，使图像更为精确清晰；

Laser Scanning Confocal Microscope ( LSCM ) excludes the out-of-focus signals effectively and enhances its resolution and contrast and provides clearer image .

激光扫描共聚焦显微镜（LSCM）分析表明，SOD在细胞内呈从细胞中心到细胞膜浓度逐步下降的辐射分布特征。

Laser scanning confocal microscopic ( LSCM ) assay indicated that SOD were distributed in a regular way of decreasing SOD concentration from central to peripheral areas of the carrier cells .

方法分离纯化乳鼠心成纤维细胞，免疫荧光双标记技术进行了Vim和Mi的标记，激光扫描共聚焦显微镜观察。

METHODS The cardiac fibroblasts of rat underwent double labeling of Vim and Mi by immunofluorescence technique after isolation and purification , then were observed with laser scanning confocal microscope .

方法：用Fluo3AM荧光标记技术和激光扫描共聚焦显微镜检测不同因素处理的A549肺上皮细胞[Ca2+]i。

Methods : Fluo-3 / AM fluorescence technique and laser scanning confocal microscope were used to measure the intracellular calcium level after different reagents were added in Hanks of A549 cells .

应用荧光双重标记免疫组织化学方法和激光扫描共聚焦显微镜技术对足三里穴、非经非穴处皮肤组织Cx43进行定位、定量分析。

Quantity and distribution of Cx43 on skin tissue of Zusanli and non-channel-non-acupoint were detected by immunohistochemistry with a fluorescence double-label technique and lasser scanning confocal microscope .

运用激光扫描共聚焦显微镜术观察到，在胃癌细胞BGC亿n中，KAll儿D82蛋白定位在细胞质膜周围，细胞浆和细胞核中没有表达；

Under the observation of Laser-Scanning Confocal microscope , location of KAI1 / CD82 protein in BGC-823 cells was clearly visualized in the area of cell membrane ;

方法：体外培养人牙龈成纤维细胞，钙离子指示剂Fluo-3染色，激光扫描共聚焦显微镜下观察加入不同浓度硝苯地平（NIF终浓度分别为1200、32.4ng/mL）后荧光亮度的变化。

Methods : Gingival fibroblast cells were cultured in vitro , then dyed with Fluo-3.The changes of light density of cells under confocal laser microscopy were investigated .

采用倒置显微镜、荧光显微镜、透射电镜和激光扫描共聚焦显微镜观察到TCPC和RPC作用4d后的Hela细胞呈典型的凋亡形态。

After Hela cells were treated with TCPC and RPC for 4d , typical apoptotic morphological : changes could be observed by inverted microscope , fluorescence microscope , transmission electron microscope ( TEM ) and LSCM .

肝癌细胞及正常肝细胞中间隙连接蛋白Cx32、Cx43的表达&激光扫描共聚焦显微镜研究

Expression of gap junction proteins Cx32 、 Cx43 in human hepatocarcinoma cell lines and normal liver cell line : study with laser scanning confocal microscope

方法场强为6×104V/m的EMP照射恒河猴，采用Feulgen、AgNOR、PI染色方法，以激光扫描共聚焦显微镜和图像分析技术对眼辐射损伤进行检测。

Methods Rhesus monkeys were irradiated with 6 × 10 4 V / m of EMP , and Feulgen , AgNOR , PI staining and LSCM , image analysis were all used to detect and analyze the pathological changes .

用Fluo－3／AM进行染色，在37℃、5%CO2的孵育箱内孵育60分钟，用激光扫描共聚焦显微镜进行荧光监测。

The cells were incubated with fluo-3 / AM at 37 ℃, 5 % CO 2 for 60 minutes . Fluoresence was measured under the laser scanning confocal microscope .

方法：高场强（2～12）×104V/m的EMP、1～30次辐照实验犬，采用Feulgen，AgNOR和PI染色方法，以激光扫描共聚焦显微镜（LSCM）和图像分析技术进行检测。

Methods : Dogs were irradiated with ( 2-12 )× 10 4 V / m of EMP for 1 to 30 times . Feulgen , AgNOR and PI staining , LSCM and image analysis were used to detect and analyze the pathological changes .

结束后，经下腔静脉取血并分离血清。采用盲法用上述10%血清培养HSCs24h并负载好Fluo-3/AM后使用激光扫描共聚焦显微镜（LSCM）检测HSCs[Ca2+]i。

The blind method was used to cultivate HSCs by using the above 10 % serum and fluo-3 / AM was loaded , HSCs [ Ca 2 + ] I was examined with laser scanning confocal microscopy ( LSCM ) .

干预：分别给PBN及舌咽和迷走神经内注射四甲基罗丹明（tetramethylrhodamine-dextran，TMR），生物素化葡聚糖胺（biotinylateddextran-amine，BDA）并进行NOS免疫组织化学反应，结合激光扫描共聚焦显微镜观察。

INTERVENTIONS : Tetramethyl rhodamine - dextran ( TMR ) and biotinylated dextran - amine ( BDA ) were injected into the PBN and vago - glossopharyngeal nerves respectively combining with the immunohistochemical method for NOS and the observation with laser confocal scanning microscope .

目的：研究得力生注射液对胃癌细胞P-gp、GST-π表达的影响。方法：采用免疫荧光结合激光扫描共聚焦显微镜技术观察胃癌细胞株SGC-7901P-gp、GST-π表达。

Objective : To study the effect of De Li Sheng to P-gp 、 GST - π expression in stomach cancer cells SGC-7901 . Methods : To observe P-gp 、 GST - π expression on SGC-7901 with immunity fluorescence and laser scanning confocal microscopy ( CLSM ) .