渗滤法

A组溶血性链球菌膜抗原吸附心肌抗体金标免疫渗滤法的建立

To Establish DIGFA with Membrane Antigen of A Group Hemolytic Streptococcus Adsorbed Myocardial Antibody

结果表明金探针具有很强的选择性，斑点免疫金渗滤法能准确快速地检测出多克隆抗体的特异性。

The results show the gold-probe possesses high selectivity and DIGFA is capable of measuring the specificity of polyclonal antibodies accurately and rapidly .

用斑点金免疫渗滤法检测患者血清抗HPylori一cagA一IgG抗体。

Dot immunogold filtration assay was used to detect H pylori-CagA-IgG antibody .

应用斑点免疫金渗滤法检测旋毛虫病患者血清IgG

Application of Dot-immunogold Filtration Assay in Detection of IgG in Sera of Trichinellosis Patients

胶体金膜渗滤法测定HIV感染者唾液中分泌型IgA

Determination of salivary secretory IgA subclasses in HIV infected patiens by colloidal gold filter infiltration

目的观察渗滤法水蛭提取液对体外培养视网膜色素上皮细胞（RetinalPigmentEPithelium，RPE）增殖的影响。

OBJECTIVE To observe effect of hirudinoid extract by percolation leech on proliferation of retinal pigment epithelium ( RPE ) cultured .

金标免疫渗滤法和ELISA检测治疗前后配对血清抗体有较好疗效评估价值。

Detection of antibodies in paired sera ( pre_ and post treatment ) of patients by DIGFA or ELISA is potential to evaluate chemotherapeutic effect .

用基因工程重组的丙肝抗原包被于硝酸纤维素膜，建立了检测丙肝IgG抗体的斑点免疫渗滤法。

A dot immunofiltration assay ( DIFA ) of detecting HCV IgG antibody was established by coating recombined HCV antigen on the nitrocellulose membrane .

斑点免疫金渗滤法（DotImmunogoldFiltrationAssay，DIGFA）具有操作简便、结果稳定的特点，是目前血清学检验中较为灵敏和快速的方法。

Dot immunogold filtration assay ( DIGFA ) is more sensitive and rapid method in serum test with simple protocol and stable results .

以鸡卵核42000蛋白为抗原的斑点金免疫渗滤法的建立及对SLE血清学诊断的评价

Development and evaluation of a dot immunogold filtration assay for the serodiagnosis of systemic lupus erythematosus using 42-kD nuclear protein of chicken egg as antigen

方法采用半定量的胶体金免疫渗滤法对52例感染性腹泻患儿和20名正常儿童进行PCT检测。

Methods The PCT level of 52 children with the infectious diarrhea and 20 healthy children was measured by a semi-quantitative colloidal gold immunofiltration assay .

目的探讨金标免疫渗滤法（DIGFA）检测并殖吸虫抗体的临床诊断价值。

Objectiv To evaluate the usefulness of dot immuno-gold filtration assay ( DIGFA ) for the diagnosis of Paragonimus infection .

为建立简便、快速的斑点金免疫渗滤法（DIGFA）检测肿瘤患者血清弓形虫IgG抗体。

To establish a simple and rapid method with Immunogold filtration assay ( DIGFA ) for the detection of Toxoplasma gondii antibody IgG in serum of the tumor patients .

目的建立一种伴有质控点的金免疫渗滤法（GIFA），用于半定量检测血清甲胎蛋白（AFP）。

Objective To develop a semi-quantitative gold immunofiltration assay ( GIFA ) with quality control dots for the detection of alpha-fetoprotein ( AFP ) in serum .

以血吸虫虫卵浸出液为抗原，金标记兔抗人IgG为显示剂，建立了检测抗血吸虫抗体的金标免疫渗滤法（DIGFA）。

Dot immunogold filtration assay ( DIGFA ) was established to detect anti schistosome antibodies , using schistosome egg extract as antigen and rabbit anti human IgG labelled with colloidal gold as probe .

目的利用针对沙门氏菌O9抗原单克隆抗体3-47-0和胶体金标记亲和纯化的羊抗鼠IgG，建立一种以微孔滤膜为固相载体，快速检测沙门氏菌O9抗原的斑点免疫金渗滤法（DIGFA）。

The dot immuno-gold filtration assay ( DIGFA ) by using monoclonal antibody 3-47-0 and colloidal gold labeled with affinity purified goat anti-mouse IgG was developed for the rapid detection of O_9 antigen of Salmonella .

目的建立金标免疫渗滤法（DIGFA）检测A组溶血性链球菌膜抗原吸附心肌抗体（HRA），探讨其对风湿性心脏病诊断的意义。

Objective To establish DIGFA to examine HRA absorbed by membrane antigen of A group hemolytic streptococcus and study the value for diagnosis of rheumatic heart disease using this method .

酵母重组表达SS-B抗原的纯化和快速检测抗SS-B抗体斑点免疫金渗滤法的建立

Purification of Recombined SSB Antigen Expressed in Pichia Pastoris and Establishment of Dot Immunogold Filtration Assay to Detect Anti-SSB Antibody

斑点免疫金渗滤法检测胸水抗PPD-IgG对结核性胸腔积液的诊断价值

The diagnostic value of detection of Anti-PPD-IgG in pleural effusion for tuberculous pleural effusion by dot-immunogold filtration assay

通过优化抗原包被浓度、封闭液和金标SPA工作浓度等建立了能够区分口蹄疫自然感染动物和免疫动物的斑点免疫金渗滤法和胶体金免疫层析试纸法。

The research established DIGFA and GICA witch could differentiate the natural infected animals from vaccinated animals by the way of optimizing the concentration of antigen coated on the NC film , the confining liquid and the concentration of colloidal gold labelled with SPA .

方法采用斑点免疫金渗滤法定性检测235例患儿标本[包括血清207份、脑脊液（CSF）43份、胸水8份、腹水5份、胃液4份]中抗结核LAM-B+相对分子质量为38-IgG。

Methods LAM-B + 38 kD-IgG in 207 serum samples , 43 cerebrospinal fluid , 8 pleural fluid , 5 ascitic fluid , 4 gastric juice specimens were obtained from 235 children with tuberculosis and non-tuberculosis were detected by the dot immunogold filtration assay ( DIGFA ) .

方法采用间接酶联免疫吸附试验（ELISA）和胶体金免疫渗滤法（DIGFA）同时对218例蚌埠地区无偿献血员血清幽门螺杆菌抗体（HP-IgG）进行检测，双阳性者确定为HP感染。

Methods Enzyme linked immunosorbent assay ( ELISA ) and dot immunogold filtration assay ( DIGFA ) were applied to detect anti-HP in the serum of 218 blood donors . The positive subject who was screened by two methods was regarded as HP infector .

目的探讨成虫三氯醋酸抽提抗原（AWA-TCA）金标免疫渗滤法在日本血吸虫病诊断和疗效考核中的潜在应用价值。

Objective To investigate the potential of diagnosis and therapeutic efficacy of dot immunogold filtration assay for schistosomiasis japonica using AWA-TCA as antigen .

测定方法：血浆FIB采用凝固法原理，高浓度凝血酶存在时，待测稀释血浆的凝固时间与其FIB含量成反比关系；血D-二聚体应用免疫渗滤法原理。

Measurement method : Plasma FIB value was measured by coagulation theory . When there is a high concentration of thrombin , clotting time of Diluted test plasma levels is inversely proportional to its FIB . Blood D-dimer were tested by immunogold filtration assay principle . 4 .

斑点金免疫渗滤法检测大肠杆菌的研究

The detecting research of Enterobacteriaceae by the dot immunogold filtration assay

植物&土壤渗滤法对农村生活污水降解研究

The Degrade Effect of Plant-soil Infiltration System on Rural Domestic Sewage

应用斑点金免疫渗滤法诊断日本血吸虫病

Application of dot immunogold filtration assay for diagnosis of schistosomiasis japonica

斑点金免疫渗滤法在布鲁氏菌病监测地区的现场应用

Field application of dot immunogold filtration assay in brucellosis surveillance area

斑点金免疫渗滤法在检测囊虫抗体中的应用

Application of Dot Immunogold Filtration Assay in Antibody Detection for Cysticercosis

快速检测尿微量白蛋白的半定量金免疫渗滤法的研究

Studies on the semi-quantitative gold immunofiltration assay for rapid detection of microalbuminuria