洗脱剂

脱脂奶粉是廉价的蛋白质洗脱剂。

Nonfat dry milk is an inexpensive proteinaceous eluent .

最佳解吸条件为洗脱剂浓度为75%，洗脱剂pH值为3，洗脱流速1.5mL/min，洗脱剂用量为60mL，解吸率为94.6%。

The optimum desorption conditions were that concentration of eluent was 75 % , pH of eluent was 3 , elution rate 1.5mL / min , the volume of elution was 60mL , and the desorption rate was 94.6 % .

根据葛根素收率、HPLC图谱，筛选洗脱剂的浓度、洗脱方式；

According to the yield of puerarin and the figure of HPLC , the concentration of the eluent and the procedure of the elution were chosen .

以5%HCl溶液为洗脱剂，动态逆流操作条件下，Cu、Ni和Zn的洗脱率分别达到88.85%、86.15%和88.40%；

The regeneration rate of Cu , Zn and Ni respectively reaches 88.85 % , 86.15 % and 88.40 % with 5 % HCl by dynamic counter flow operation .

根据试验结果确定最佳提取条件为：上柱啤酒废酵母抽提液的最适pH值为7.0，最适洗脱剂为pH值为4.4的磷酸盐缓冲液。

According to the study , They made sure the best distill condition : the best pH of sample liquid is 7.0 and the desorption process was performed with 4.4 phosphate liquid .

分别从pH值、提取液浓度、流速、不同种类的洗脱剂、不同浓度乙醇洗脱剂等方面考察并优化了该树脂的吸附和洗脱工艺条件。

Extract concentration , pH , flow rate , different eluants , eluants of different ethanol concentration and other factors were investigated . The adsorption and desorption crafts of DM-130 macroporous resin were optimized .

根据不同的产物，柱层析的洗脱剂V（石油醚）：V（乙酸乙酷）从10：1到30：1不等。

Chromatography on silica gel with petroleum ether and ethyl acetate as eluent which ranges from 10:1 ( v : v ) to 30:1 ( v : v ) afforded oxime ether .

结果显示，在薄层硅胶H为填充剂的干层析柱上，以石油醚-乙酸乙酯（15∶1）为洗脱剂，可以将大黄酚和大黄素甲醚分离。

The results indicated that they could be separated through silica gel H for thin layer chromatography as the stational phase , and petroleum aether and ethyl acetate ( 15 ∶ 1 ) as the mobile phase .

最佳洗脱剂为70%乙醇溶液，洗脱液pH值为8.0，洗脱速率为2BV/h，洗脱体积为4BV。纯化得到的样品柠檬苦素类物质含量为37.26%。

And the optimum elution conditions were as follows : elute volume 4 BV , elute concentration 70 % , elute rate 2 BV / h , pH value 8.0.The purity of the obtained limonoids was 37.26 % .

pH约为10、体积分数为60%的乙醇水溶液为最佳洗脱剂；当每小时通过的洗脱剂体积为树脂体积的1~2倍时，洗脱率可达85%以上。

The optimal eluting solvent is 60 % ( volume fraction ) ethanol-water solution with pH value of 10 . The desorption rate overpasses 85 % when the flow velocity is 12 times of resins volume per hour .

结果表明：影响EGCG分离、纯化因素的主次顺序为：层析介质＞洗脱剂＞洗脱剂pH值＞洗脱剂浓度；

The result showed that the order for isolation and purification of EGCG was chromatography medium > eluting reagent > the pH value of eluting reagent > concentration of eluting reagent .

AB-8树脂是吸附分离纯化黑莓红色素的优良材料，洗脱剂以80%乙醇较佳。

Resin AB-8 was excellent for the separation and purification of blackberry red pigment and 80 % ethanol solution was the better elute solution .

L-1pH7.2磷酸盐缓冲液作吸附与平衡介质，80%（体积分数）甲醇水作洗脱剂。结果表明，在实验条件下，IAC柱对氯磺隆的动态柱容量达2.6！

The conditions of IAC were optimized , pH7.2 phosphate buffer was used as equilibrium and absorbent medium , 80 % methanol was used as eluent .

以氯化腐植酸溶液为模型，研究了树脂型号、水样pH值和洗脱剂对XAD树脂浓集有机致突变物的影响。

With the model of chlorinated humic acids , we studied the effects of resin type , water pH and desorbents on the efficiencies of XAD resins to recover organic mutagenic materials .

利用S.Tag~（TM）rEK纯化试剂盒对目的产物进行了纯化，并对rEK和MgCl2两种洗脱剂的洗脱效果进行了比较。

S · Tag ~ ( TM ) Purification Kit was used in the purification experiment and the two kinds of eluent ( rEK and MgCl_2 ) were compared .

每克聚酰胺吸附25mgEGCG，去离水及5%乙醇除咖啡因等杂质后，80%乙醇为洗脱剂，使EGCG含量提高至53.61%，洗脱率为68.23%。

The content of EGCG was 53.61 % and the desorption ratio was 68.23 % . The deionized water and 5 % ethanol were used to elute the caffeine . Then 80 % ethanol was used as elution .

以乙醇-水和异丙醇-水体系作为洗脱剂，得到纯度80%的DCPC溶液。

By eluting the carbon cake with ethanol-water system or isopropanol-water system , a DCPC solution of 80 % purity was obtained .

解吸时，宜采用pH2.0、80%的乙醇为洗脱剂，洗脱流速为0.5ml/min，此条件下动态洗脱率为91.25%。

While 80 % ethanol with pH2.0 as eluent , flow rate at 0.5ml / min , the desorption rate was 91.25 % .

结果表明，极性化合物用GDX502，非极性化合物用GDX102为吸附剂，以甲醇或乙腈为洗脱剂能获满意的结果。

The results show that GDX-502 and GDX - 102 are suitably used as the adsorbents on polar and nonpolar compounds , respectively . Methanol and acetonitrile are used as the eluents .

采用丙酮做洗脱剂，洗脱率为97.5%，动态吸附最佳吸附pH为9.5，吸附流速为6BV/h，穿透吸附容量为21.2mg/ml（wetresin），洗脱流速1.5BV/h。

A recovery rate of 97.5 % can be obtained with acetone as eluent . The best condition for dynamic adsorption is pH 9.5 , flow rate 6 BV / h , with a dynamic adsorption capacity achieving 21.2mg/ml wet resin , and 1.5 BV / h for desorption .

结果表明，当用Cl8和GDX－502为吸附剂、吸附速度为15～20mL·min－1时，以10mL无水乙醇为洗脱剂，回收率为85%～91%。

The results show that when using C18 and GDX-502 as adsorbents , the speed of adsorption was 15 - 20 mL / min the eluent was alcohol of 10 mL , the percent recovery was 85 % - 91 % .

对纳米氧化锌的制备条件进行了改良，加入了乙醇作为洗脱剂，得到了粒径为20～30nm的纳米氧化锌粉体，但粒径分布不均匀。

Preparation condition of nanometer zinc oxide is improved , alcohol is added as the washing agent , the nanometer zinc oxide whose particle size is about 20 ~ 30nm is prepared , but it is pocket .

油茶籽的最佳分离条件为：70%乙醇为洗脱剂，在0.8mL/min的流速下洗脱，可以分离出四种组分。

The optimal purification conditions of oil-tea seeds t separation was 70 % ethanol by the flow rate at 0.8mL / min , and four components can be separated .

以GDX-101为富集剂，丙酮为洗脱剂，最大吸附容量时，吸附效率均在90%以上，解吸效率达100%。

When it is up to maximum adsorption capacity , GDX-101 and acetone are respectively used as the concentrating and elutriating agents , all adsorbing efficiency being over 90 % and desorption efficiency 100 % .

应用单因素试验检验正交实验，结果表明，采用聚酰胺作层析介质，洗脱剂为丙酮，洗脱剂pH3.5，洗脱剂流速1.0mL/min时效果最佳。

Through the experiment of a single factor , the best composition for the agent was polyamide as chromatography medium , the acetone as eluting reagent , and the pH value 3.5 , and the flowing speed of the eluting reagent 1.0 mL / min.

S-8树脂吸附纯化的优化工艺为：吸附剂用量为总黄酮质量的10倍，以70%乙醇为洗脱剂，流速为1.5BV/h，所得产物的总黄酮含量及收率分别为82.45%，93.02%。

The optimization process of S-8 resin adsorption purification as follows : adsorbent dosage was ten times of flavonoids , 70 % ethanol as eluant , flow rate was 1.5 BV / h , and the total flavonoid content and yields of the product were 82.45 % , 93.02 % .

选取H-103大孔树脂吸附京尼平，上柱液流速为2mL/min，以60%乙醇水溶液作为洗脱剂，经重结晶纯化后获得纯度达98.2%的京尼平，整个工艺收率为77.8%。

H-103 macroporous resin was used to separate genipin and the flow rate was 2 mL / min. The genipin was eluted by 60 % alcohol solution . The purity of genipin was 98.2 % , and the yield of genipin in the whole separation process reached 77.8 % .

黄酮化合物树脂洗脱剂的纳滤回收研究

Study on the Recovery of Eluting Solvent of Flavonoids by Nanofiltration

脱脂奶粉是廉价的蛋白质洗脱剂。高磷铁水脱硅粉剂和工艺参数的研究

Research on Desilication Agent and Processing Parameter of High Phosphorus Hot Metal

乙醇水溶液是适宜的洗脱剂。

Ethanol solution is a kind of efficient eluent .