薄层层析

薄层层析定量测定方法在抗生素组份测定中的应用Ⅱ.红霉素组份薄层层析定量测定方法及应用

The application of TLC in determination of antibiotic components ⅱ . the method and application of TLC in determination of contents of erythromycin components

常规检测AFB1的方法有生物学方法、HPLC及薄层层析等方法；由于各方法的局限性，人们逐渐专注于应用免疫学方法进行检测。

Though there are some conventional detection methods such as biological method , HPLC and TLC , due to the limitations of each method , application of immunological method is gradually attracting researchers ' interest .

方法：用硅胶柱层析法对平贝总碱进行分离纯化，固定相为薄层层析硅胶G，流动相为乙酸乙酯-甲醇-氨水（17：2：1）。

The solid phase was thin layer chromatography silica gel G and the mobile phase was ethyl acetate-methanol-ammonia ( 17:2:1 ) .

本文报道以薄层层析为先导，用柱层析法分离磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）。

In this paper it is reported that using TLC PC and PE are first separated by column chromatography further .

根据吸收光谱和薄层层析分离后显色斑点的Rf值及荧光颜色，在雌雄同株的瓠瓜植株中鉴定出了甾类性激素雌酮。

Estrone , a steroid hormone , is identified in the monoecious Cucurbitaceous plant , Lagenaria leucantha Rusby .

同时对不同溶剂提取的黄酮类化合物做了薄层层析（TLC）的研究。

Flavonoid extracted with different solvents were also studied by TLC ( thin-layer chromatography ) .

叶、皮和根部芳香精油的成分，同时运用薄层层析（TLC）筛分法对其抗氧化活性物质进行了对比分析。

TLC-screening method was used for analysis of antioxidant components in the oils .

该抗菌活性物质具有较好的热稳定性、pH稳定性，易溶于氯仿，经薄层层析展开后在紫外光下具有发荧光的特点，纸电泳实验证实该物质是一种弱酸性物质。

After developed on thin layer chromatography it possesses the property of fluorescence . Paper electrophoresis has proved that it is a substance of weak acidity .

本研究通过薄层层析HPLC方法着重分析了猴头菌的腺苷成分，并对猴头菌的药物制品-胃乐宁浸膏的组成成分和矿质元素进行了测定。

To study the composition with medicinal effects of Hericium erinaceus , we mainly analyzed the adenosine in Hericium erinaceus using TLC and HPLC .

结果以薄层层析法制备麦白霉素组分，得到的未知组分纯度符合NMR鉴定结构的要求。

Results The purity of the unknown component prepared by TLC method met the requirements of the structure identification by NMR method .

多糖的薄层层析、GC和GC-MS分析，均表明其主要由葡萄糖、半乳糖和甘露糖组成。

The thin layer chromatography ( TLC ), GC and GC-MS all showed BF was consisted by glucose , galactose and mannose .

样品通过薄层层析以及600~190nm波长扫描定性鉴定后，用高效液相色谱法（HPLC）检测纯度，结果表明纯度为97.6%。

After identified by TLC and 600 ~ 190 nm wavelength scan , purity of the sample was measured by HPLC , and the purity was 97.6 %

硅胶板薄层层析方法显示纯化的磷脂酶酶C和标准磷脂酶C（Clostridium.perfringens）对磷脂酰胆碱底物的水解产物相同，即该酶的水解特性与标准磷脂酶C相一致，表明该酶属于磷脂酶C。

The hydrolysis products profiles of the purified enzyme and standard phospholipase C ( Clostridium perfringens ) by thin layer chromatography were the same , suggesting that the purified enzyme belonged to the PLC family .

通过薄层层析（TLC）、高效液相色谱（HPLC）分析，该多糖主要由葡萄糖、半乳糖、甘露糖组成。

It was consisted of glucose , galactose and mannose with the thin layer chromatography ( TLC ) and high-performance liquid chromatography ( HPLC ) analysis .

采用有机溶剂提取总磷脂，高效薄层层析分离PC和PE，进一步用不同展开体系分离纯化非缩醛磷脂和缩醛磷脂。

The total phospholipids were extracted and PC , PE were separated as above , non - plasmalogens and plasmalogens were separated and purified by the method of HPTLC with different developing system .

采用免疫沉淀、薄层层析及γ液闪计数方法检测PI3K的活性，并进行分析比较。

PI-3 kinase activity was detected by immunoprecipitation , thin-layer chromatography and gamma scintillation counting . The results were analyzed by statistical methods .

方法：通过抑菌作用实验，小鼠的急性毒性实验，小鼠耳急性炎症实验，巯基的化学鉴别和薄层层析，研究二巯丙磺钠对ET的化学性拮抗作用。

METHODS : Experiments of antibacterial action , acute toxicity on mice , acute inflammation on ears of mice , sulfhydryl groups detection and thin_layer chromatography were made to study the chemical antagonism of Na_DMPS on ET.

薄层层析硅胶GF254为固定相，乙酸乙酯甲醇水（7-2-1，v/v/v）为展开剂，熊果苷和氢醌的Rf值分别为0.56和0.67。

The optimal conditions of TLC were as follows : silica gel GF254 as stationary phase , ethyl actate-methanol-water ( 7-2-1 , v / v / v ) as mobile phase .

干胶板CS&9000型薄层层析扫描仪扫描结果表明，各热击时间合成HSP的累积量华北夏大豆明显多于东北春大豆。

Hsp accumulation of summer soybean in North China was obviously more than that of spring soybean in Northeast according to scanning dry gelationous plate with CS-9000 scanner .

方法：采用除蛋白、除色素、回收乙醇、盐析、萃取等生物化学方法，从羊胆汁中分离提取GCA，采用了吸附色谱法和薄层层析色谱法分离纯化和鉴定了GCA。

Isolation of Lycopene Isomers Using HPLC Method Crude extract of GCA went through deproteinization , depigmentation , ethanol reclamation , salting-out , extraction and anhydration .

应用土壤薄层层析（TLC）法测定了14C－绿黄隆在10种土壤中的移动率，其Rf值为0.65～1.0，表明绿黄隆在供试土壤中分别属可移动性和极易移动性。

The migration of 14C-chlorsulfuron in 10 kinds of soil was tested by means of soil TLC . 14C-chlorsulfuron with Rf values between 0.65 and 1.0 belongs to mobile and easily mobile herbicide .

采用硫酸、乙醇等提取，SephadexG50及RPHPLC技术纯化，从苦瓜中分离出苦瓜多肽P，又称植物胰岛素，该技术优于过去的薄层层析纯化方法。

P-polypeptide , that was so called plant insulin , was extracted from Momordica charantia seeds with vitriol and ethanol and purified with Sephadex G-50 gel filtration and RP-HPLC . The technique was better than thin-layer chromatography technology .

本文报道用薄层层析紫外分光光度法测定蜂王浆制剂中10-羟基-△~2-癸烯酸（10-HDA）的含量。

This paper reports the determination of 10-hydroxy-trans - △ ~ 2-decenoic acid ( 10-HDA ) levels in royal jelly preparations using TLC-UV spectrophotometry .

薄层层析检测得知ABM主要由葡萄糖和半乳糖组成，紫外和红外光谱表明，ABM中含有核酸物质，并含有少量结合蛋白。

The components of ABM were identified by paper chromatography and UV and IR spectrum , and it was composed of D-Glc , D-Gal , nucleic acid , and a little protein .

低聚木糖（XOs）薄层层析显示，苹果木聚糖经酶解的主要产物是X2、X3，且X3的含量大于X2的含量。

Thin-layer chromatography ( TLC ) of XOs showed that the main enzymatic hydrolyzate of apple xylan were X3 and X2 , and the content of the X3 was large than X2 .

在蒸气浴条件下，T-2毒素与琥珀酸酐（HS）反应合成了T-2HS，薄层层析显示，目标半抗原合成成功；

First , T-2 toxin reacted with succinic anhydride in steam-bath to synthesize T-2 hemisuccinate ( T-2HS ) . Thin layer chromatography analysis showed that the objective hapten was obtained successfully .

经~1H-NMR、~（13）C-NMR及FD-MS，EI-MS分析、薄层层析行为与标准品对照，证明抗生素83-1050B组分Ⅰ、Ⅱ分别与阿普拉霉素和妥布拉霉素同质。

The ~ 1 H-NMR , ~ ( 13 ) C-NMR , FD-MS and EI-MS analyses and the TLC behaviors indicate that 83-1050B-I , III are identical with Apramycin and Tobramycin .

方法：真菌与不同浓度氟康唑温育24h后计数、称量、皂化，提取真菌中难皂化脂，薄层层析，扫描测定其中的麦角固醇和羊毛固醇含量。

Methods : Saponification and extraction of the nonsaponifiable lipids ( NSLs ) of fungi were carried out after 24 h of incubation with varied concentrations of fluconazole . The components of the NSLs were separated by TLC and the concentrations of ergosterol and lanosterol were determined .

发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。

Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent , silica gel column chromatography and thin-layer chromatography .

取饲喂含2%钩吻粉的基础日粮30d、60d的肉鸡肝脏及肌肉进行薄层层析分析与气相色谱分析，都未检测得到钩吻碱。

Ge were not found in the liver and muscle of the broilers fed the basic diet adding 2 % Ge powder in 30 days and 60 days which were detected by thin-layer chromatography and gas chromatography .