流动相

采用梯度洗脱及蒸发光散射检测器（ELSD），流动相为氯仿-异丙醇-水系统。

Evaporative light scattering detector ( HPLC-ELSD ) and gradient eluted program were used and the mobile phase system was chloroform isopropanol water .

在此基础上，本文讨论了ESI和APCI这两种离子化方式的选择原则，流动相的选择及流速匹配、干燥气的流速和温度确定等实践中遇到的问题。

Practical problems such as the selection of ESI or APCI , the selection of mobile phase , the flow rate and temperature of drying gas were also discussed .

以蒸馏水为流动相B；

Taking distilled water as mobile phase .

流动相：A相为乙腈，B相为水，梯度洗脱；

Mobile phase was A : ACN , B : H _ 2O , gradient elution ;

流动相：0·1%甲酸水溶液（A）-乙腈（B），梯度洗脱；

Mobile phase : 0.1 % formic acid-water ( A ) and acetonitrile ( B ), gradient elute ;

以液体CO2作流动相的加压液相色谱探讨

Approach to HPLC Using CO_2 as Mobile Phase

介绍了采用高效液相色谱法，通过低浓度的草酸流动相，测定菠萝中维生素C含量的方法。

Determination of vitamin C in pineapple by HPLC with low concentration of oxalic acid as flow phase is presented .

以甲醇和0.1%乙酸铵溶液为HPLC的流动相；

The mobile phase of HPLC was methanol-0.1 % ammonium acetate aqueous solution .

文中着重探讨了影响pH区带逆流色谱分离效果的流动相流速、逆流色谱转速、溶剂组成、样品量和酸碱度等主要影响因素。

The main factors for the separative effect of pH-zone-refining Countercurrent Chromatography are discussed in this paper .

手性流动相HPLC法拆分萘普生对映体的研究

Study on resolution of racemic naproxen with chiral additive as mobile phase by RP-HPLC

青霉素V钾片含量测定中流动相影响因素的探讨

Exploration on the influencing factors of mobile phase in the content determination of Penicillin V Potassium Tablets

3种新型α1-受体阻断剂的手性流动相HPLC分离与制备

Chiral separation and preparation of three new antagonists of α _1-adrenoceptors by chiral mobile phase HPLC

用SEC柱和胶束流动相分离小分子的模型

Separation Model of Small Molecules with SEC Columns and Micellar Mobile Phase

流动相组成、浓度和pH对蛋白质在金属螯合柱上的保留特性的影响

Influences of the Mobile Phase Constitution , Salt Concentration and pH Value on Retention Characters of Proteins on the Metal Chelate Column

β-环糊精手性流动相HPLC法拆分α-环己基扁桃酸对映体

Resolution of α - Cyclohexyl-mandelic Acid into Enantiomers by HPLC with β - Cyclodextrin as Additive in Mobile Phase

目的：建立HPLC手性流动相添加剂法拆分美托洛尔对映体的方法。

Objective : To establish an HPLC method for the resolution of metoprolol enantiomer using chiral mobile phase additives .

流动相A、B均为甲醇-水（12∶88、43∶57）；

The mobile phase consisted of methanol-water ( 12 ∶ 88 and ( 43 ∶ 57 ,) v / v , for gradient A and B ) .

该方法消除了流动相的体积梯度，使得Rf值更接近比移率R，为色谱基本理论在薄层色谱技术中的应用和柱色谱与平板色谱数据的互换创造了条件。

Therefore , the condition for applying chromatography principle in TLC and exchanging the data between column and planar chromatography was established .

通过考察色谱柱、柱温和流动相组成等因素对MC分离的影响，优化了色谱条件。

The conditions of chromatography was optimized by examining column , temperature and mobile phase composition .

长效甾体避孕药RP-HPLC流动相选择性优化与定量分析

Optimization of mobile phase composition for RP-HPLC separation and determination of long acting steroidal contraceptives

根据色谱保留值与流动相pH值的函数关系对212种常用药物的分类

The Classification of 212 Drugs in Terms of Chromatographic Retention Value as a Function of the pH Value of Mobile Phase in Reversed-Phase High Performance Liquid Chromatography

以甲醇和水为二元流动相，用包括碱性、酸性和中性有机化合物在内的混合物对该固定相进行评价，并考察了该填料的适用pH范围及水解稳定性。

Chromatographic evaluations were carried out by using a mixture of organic compounds including acidic , basic and neutral analytes and methanol water as binary mobile phase .

用高效液相色谱法对橙皮苷进行含量测定，采用ODS柱，流动相为甲醇-水（40∶60）。

The mobile phase was methanol-water solution ( 40 ∶ 60 ) .

目的利用计算机的VISUALBASIC60编程技术，编制了利用四面体法优化高效液相流动相组成的程序。

Objective Using the Visual Basic 6.0 program technique to compile the procedure of the tetrahedron method for the optimization of the mobile phase by the high performance liquid chromatography .

流动相为甲醇-水-三乙胺-磷酸（700∶300∶5∶1.5），用磷酸（1→10）调pH至7.3±0.1；

The mobile phase was consisted of methanol-water-triethylamine-phosphoric acid ( 700 ∶ 300 ∶ 5 ∶ 1.5 ), with the pH of 7.3 ± 0.1 adjusted with phosphoric acid .

流动相A为1%的三氟乙酸溶液，B为乙腈-甲醇（75：25）；

The mobile phase was consisted of 1 % trifluoroacetic acid solution as mobile phase A and acetonitrile - methanol ( 75:25 ) as mobile phase B with UV detection at 275 nm .

以HPLC法碱性流动相建立的指纹图谱可以作为川黄连质量控制的方法之一，并可以进行含量测定。

HPLC with the basic mobile phase can be used as one of the methods to control the huanglian quality , and to determine the content .

使用HPLC，利用在给定的色谱柱和流动相下化合物具有不同移动速率的事实可以实现化学分离。

Chemical Separations can be accomplished using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase .

考察了流动相组成、有机改进剂含量、离子对试剂浓度及pH值对色谱分离的影响。

The effects of the composition of the mobile phase , content of organic solvent , concentration of the ion pair reagent and PH value on chromatographic separation were investigated .

HPLC分析结果可见，以甲醇为流动相，他们早早被洗脱下来，吸收峰出现时间较早。

The results of HPLC analysis were : using methanol as the mobile phase , it could be eluded down early , and the absorption peaks appeared earlier .