抗酸染色法

方法昆明株小鼠经地塞米松免疫抑制10d后，取粪便涂片、改良抗酸染色法检测小鼠隐孢子虫卵囊。

Methods Oocysts were collected from KM mice ( immunodepressed by dexamethasone for 10 days ) and examined with modified acid-fast staining .

介绍一种改良的抗酸染色法

Introducing an improved acid-fast staining method

结果结核病组65例病人的临床样品，抗酸染色法阳性9例，抗体测定法阳性47例，PCR杂交法阳性53例；

Results Among 65 tuberculosis cases , the positive results of acid fast stain , serum antibody detection and PCR hybridization were 9 , 47 and 53 respectively .

结果：PCR检测法敏感性为48.8%，荧光染色法为44.2%，细菌培养为32.6%，抗酸染色法为25.6%。

Results : The sensitivity were 48.8 % by PCR , 44.2 % by fluorescent staining 32.6 % by culture and 25.6 % by acid fast staining , respectively .

目的探讨抗酸染色法、抗体测定法、PCR杂交法在临床结核病快速诊断中的作用与地位。

Objective To explore the role and situation of three rapid diagnosis methods , i.e. acid fast stain , serum antibody detection and PCR hybridization , in rapid diagnosis of clinical tuberculosis .

方法快速改良抗酸染色法检测粪便中的C.目的：探讨新生儿医院感染率与住院费用相关因素分析。

Methods : The incidence of C. Objective : The purpose of the study was to explore the relationship between the incidence of the newborn infection in hospital and the cost .

[方法]采用改良抗酸染色法筛选，间接免疫荧光法（IFA）确定隐孢子虫感染情况。

[ Methods ] A direct modified acid-fast stain method , a monoclonal antibody-based indirect immuno fluorescence assay ( IFA ) were used .

方法我们采用改良抗酸染色法（IK）和聚合酶链反应（PCR）法对30例病理学诊断为非特异性淋巴结炎和15例正常胎儿石蜡包埋组织重新切片进行检测分析。

Method : Paraffin embeded tissue from 30 cases of pathologically diagnosed non-specific lymphadenitis and 15 normal embryo were resectioned and examined with both methods of IK and PCR .

经χ2检验，上述5项指标抗体测定法和PCR杂交法均显著高于抗酸染色法（P0.01），但抗体测定法和PCR杂法之间无显著性差异（P0.05）。

By χ 2 test , the five indexes of antibody detection and PCR hybridization were higher than that of acid fast stain ( P 0.01 ) . However , there was no significant difference between serum antibody and PCR hybridization ( P 0.05 ) .

方法：应用改良抗酸染色法及免疫酶染色法，对96例经纤维支气管镜活检标本及20例手术切除肺标本进行结核菌及其L型的检测。

Methods : Intensified Kinyoun 's acid fast staining and immunoenzyme staining were used for examining in 96 specimens obtained from fibroptic bronchoscopic biopsy and 20 specimens obtained from pulmonectomy .

金胺-酚和改良抗酸染色法联用时，方法简便，效果好。

The best result could be obtained when auramine-phenol staining method was first used and folloWed by modified acid-fast staining method .

方法：取患婴和家人以及所饲养的牲畜的新鲜粪便，用改良抗酸染色法涂片标本检查；

Method : Fecal samples form 271 infants with diarrhea under 4 years old and their parents and the domestic animals were examined for cryptosporidium by the modified acid-fast staining .

方法采集安徽省各地市不同教育层次学生粪便标本共4048份，采用金胺-酚染色法和改良抗酸染色法检查隐孢子虫卵囊。

Methods Stool specimens of 4 048 students at different educational levels were collected , oocysts of cryptosporidium were identified with auramine - phenol stain and improved ami - acid stain .

方法：采用金胺酚染色法、改良抗酸染色法对安徽省11个地市5421份不同人群的新鲜粪膜标本进行镜检。

METHODS : The oocyst of Cryptosporidium parvum in 5421 fresh stool samples from eleven areas of Anhui province was tested by auramine phenol stain and improved anti acid stain respectively .

采用金胺-酚染色法及改良抗酸染色法染色，对青岛地区969例儿童粪便进行了隐孢子虫感染检查，共发现阳性者26例，感染率为2.7%，其中一乡村为7.9%。

Out of 969 children examined in Qingdao area , 26 were found positive , showing an overall infection rate of 2.7 % , with a maximum rate of 7.9 % in one village .

[方法]通过生理盐水直接涂片法、卢戈氏碘液染色涂片法或改良抗酸染色法对3116名腹泻病人的新鲜粪便进行镜检。

Microscope examination was carried out for fresh feces derived from 3 116 diarrhea patients by the methods of direct smear with saline , Lugol 's solution staining smear or modified acid fast staining , respectively .

采用金胺酚&改良抗酸染色法对合肥地区4个鸡场的120个粪样进行检查，结果表明，鸡隐孢子虫感染率为50.83%；

Faecal specimens from 4 chicken farms in Hefei area were examined with auramine-phenol and modified acid-fast staining method . The result showed the infective rate of chicken cryptosporidium was 50 83 % ( 61 specimens out of 120 ) .

方法对黔南少数民族聚居的山区采取整群分层抽样方法，对1739份农村居民人粪和47份动物粪便标本用改良抗酸染色法检查隐孢子虫卵囊。

Method The oocysts of Cryptosporidium parvum in the feces samples from 1739 rural residents and 47 animals were stained by modified acid-fast stain . Result The total infective rate of Cryptosporidium parvum was 2.30 % ( 40 / 1739 ) .

方法运用经典抗酸染色（ZN法）、改良抗酸染色（IK法）、免疫组织化学染色（ABC法）及TBPCR方法对330例肺癌患者结核菌感染状况进行了前瞻性研究。

Methods A prospective study on the infection of mycobacterium tuberculosis was carried out in 330 lung cancer patients by classic acid fast staining ( ZN ) and improved acid fast staining ( IK ) method and immunohistochemistry staining ( ABC ) and TB PCR methods .

方法取上述动物的粪便292份，用金胺-酚-改良抗酸复染法染色，镜检卵囊。两类改良抗酸染色法的比较观察

Method 292 feces specimen from six kinds of animals were collected and stained with Auramine phenol and re stained with modified acid fast for checking up cryptosporidial oocysts . Comparative investigation on two modified techniques of acid fast stain