琼脂扩散试验

目前确诊该病通常借助于琼脂扩散试验，PCR试验等实验室诊断。

Currently the disease is usually diagnosed by means of laboratory diagnosis , such as agar diffusion test , PCR test .

应用PEG平板进行琼脂扩散试验检测新城疫抗原，提高了对新城疫抗原的检出率。

Detecting ND antigen using agar diffusion test with PEG plate can raise the ration of ND detection .

主要采用了琼脂扩散试验、间接ELISA试验等诊断方法进行血清抗体检测。

Mainly uses agar-agar diagnosis methods and so on proliferation experiment , ELISA experiment to carry on the serum antibody examination .

SPF鸡琼脂扩散试验

SPF chicken-Agar gel precipitation test

鸡传染性法氏囊病（IBD）琼脂扩散试验（AGP）诊断试剂的研制

Development of Agar Gel Precipitation ( AGP ) Test Reagents for the Diagnosis of Infectious

双向琼脂扩散试验显示抗原抗体的结合比为1：8，间接ELISA检测到抗体效价达到1：12800。

Double immunodiffusion test showed the antigen-antibody binding ratio to be 1:8 , and the titre of antibodies was 1 : 12 800 detected by indirect ELISA . 4 .

第四次免疫后第10日腋下采血，分离出血清，以进行琼脂扩散试验和ELISA。

Another ten days passed the fourth immunization , when all the mice were killed to collect the blood , separated the serum , which could be used in Agar-gel immunodiffusion and ELISA .

对40份盲样采用琼脂扩散试验（AGP）、血凝抑制试验（HI）和反转录聚合酶链反应（RT-PCR）进行了检测。

40 blind samples were examined by agar gel precipitation ( AGP ), hemagglutination inhibitory ( HI ) test and reverse transcription-polymerase chain reaction ( RT-PCR ) .

用人工抗原免疫家兔，制备了针对槲皮素的特异性抗体，并用双向琼脂扩散试验和ELISA方法对抗体进行了鉴定。

The antibody was obtained successfully by immunizing big ear rabbits with Quercetin HS BSA , the polyclonal anti quercetin antibodies were developed , which were determined with double agar diffusion test and ELISA .

本文报道单向琼脂扩散试验及淋巴细胞转化试验对51例神经系统炎症病人（脑炎及脑膜炎）进行血清及脑脊液中C3、IgG、IgA、IgM测定。

The serum and C_3 , IgG , IgA , IgM in the cerebrospinal fluid were measured in patients with inflammation in the nervous system by single agar diffusion test and lymphocyte transformation test .

国内外有许多犬恶丝虫病检测方法的报道，如：微丝蚴检查、血凝试验、琼脂扩散试验、补体结合试验、荧光抗体标记技术、PCR法等，但效果均不理想。

So far there are no ideal diagnostic although many detection methods were reported , such as : microfilaria examination , blood clotting tests , agar diffusion test , complement fixation test , fluorescent antibody labeling , PCR method .

纯化该融合蛋白并免疫C57／BL小鼠，萃取GBS表面蛋白，双向琼脂扩散试验检测抗体水平。

Anti serum of C57 / BL mice immunized with purified fusion protein was tested by double immunodiffusion to evaluate the fusion protein 's immunogenicity .

用制备的分型血清采用琼脂扩散试验对PCR阳性的胸膜肺炎放线杆菌地方分离菌株进行了鉴定，分别为血清2、3和8型，说明抗血清可用于分离菌株的鉴定分型。

The materials and method were applied to verify five PCR positive field strains and were shown to be serotype 2 , 3 and 8 . These results revealed that the prepared serum and relevant assay could be used for serotyping field isolates .

方法用双向免疫琼脂扩散试验、试管凝集试验、ELISA（间接法）分别检测经CJ疫苗免疫后的新西兰兔血清IgG抗体效价。

Methods Double immunodiffusion test , tube agglutination test , and ELISA ( indirect ) test were respectively used to detect the IgG titers in serum of New Zealand rabbits which were immunized with the both CJ vaccines .

乳牛布氏杆菌病的血清琼脂扩散试验而AGP阳性检出率为34.8%（16/46）。

A STUDY ON AGAR GEL DIFFUSION PRECIPITATION TEST IN BOVINE BRUCELLOSIS The serum samples of affected goat were detected by IHA and agar gel precipitation ( AGP ) .

本文采用单向琼脂扩散试验（SRTD）血循环免疫复合物测定法（CiC）及淋巴细胞转化试验（LTT）对50例复发性口腔溃疡患者及50例正常人进行了系列免疫指标测定。

In this paper , we have determined series immune index of 50 patients with Aphthae ulcer ( RAU ) and 50 normal persons , using SRID , CIC and LTT .

应用胶体金免疫层析法（GICA）、血凝抑制试验（HI）和琼脂扩散试验（AGID）三种方法同时检测所采集的342份鸡血清样品，并对三者的检测结果进行比较。

253 chicken serum samples were collected to detect antibody against influenza A virus by hemagglutination inhibition ( HI ) test , gold immuno-chromatographic assay ( GICA ) and agar gel immunodiffusion ( AGID ) test .

利用免疫学方法制备了牛副结核细胞浆抗原、细胞壁超声波粉碎抗原、Sephadex-G200层析抗原和草柱亲和抗原，分别与牛副结核阳性、弱阳性及阴性血清进行琼脂扩散试验。

Bovine paratuberculosis antigen was prepared from cytoplasm and cell wall and with Sephadex-G200 and M phlei absorption methods .

提纯的抗原经SDS-PAGE电泳测定其分子量约为25000，且仅此一条蛋白带；与K88抗血清进行琼脂扩散试验只出现一条沉淀线。

The purified antigen was identified as a single protein band with a molecular weight of 25 000 through SDS-PAGE and a single precipitating line with K_ ( 88 ) antiserum in the gel double diffusion test .

犬瘟热琼脂扩散试验方法的建立及初步应用

Establishment and Preliminary Application of Agar Dif-fusion for Canine Distemper Diagnosis

应用琼脂扩散试验检测新城疫抗原

Detection of Newcastle Disease ( ND ) Antigen with Agar Diffusion test

这一结果与琼脂扩散试验检测结果一致。

The result accords with the result of AGP detection .

鸡新城疫琼脂扩散试验抗原的研究

Studies on Agar Gel Precipitin Antigen of Newcastle Disease Virus

牛隐孢子虫病的研究&免疫琼脂扩散试验

Studies on cryptosporidiosis in bovine : immunodiffusion test

敏感性为琼脂扩散试验的16倍。

The assay was 16 times more sensitive than agar-gel preci - ( pitation ) test .

琼脂扩散试验检测雏鹅新型病毒性肠炎病毒及抗体的研究

Study on the Agar Gel Precipitin Test to Detect Antigen and Antibody of the Gosling New Type Viral Enteritis

鸡马立克氏病监测技术的研究及应用乳牛布氏杆菌病的血清琼脂扩散试验

Studies and Application on Monitoring Technique of Agar - gel Precipitation ( AGP ) Test for Virulent MDV - infection of Chickens

采用鹅胚增殖病毒，以琼脂扩散试验从感染鹅胚尿囊液中检出小鹅瘟病毒抗原；

The viruses were propagated in goose embryos , the GPV antigen could be detected from allantoic fluid of infected embryos by agar gel precipitation .

副猪嗜血杆菌并得诊断主要依赖细菌分离、免疫组织化学等实验室诊断方法，而间接血凝试验、琼脂扩散试验又由于操作复杂，交叉反应严重无法基层推广使用。

The detection of Haemophilus parasuis is mainly depend on IHA , Agar Diffusion Test and IHC which have disadvantages such as cross reaction and low sensitivity .

用包涵体洗涤法纯化蛋白后透析去除尿素复性，之后将蛋白用于兔抗多克隆抗体的制备，通过琼脂扩散试验测定效价为1：8。

The fusion protein was purified by inclusion body washing method . Then the purified protein was used to immunize the rabbits for preparing polyclonal antibodies . The antibodies titer was1:8.3 .