抗原修复

结论：本实验选用淋巴组织，抗原修复液选择pH7.0,8.0柠檬酸盐缓冲液均优于其它pH值，其中pH8.0染色效果最佳。

Conclusions : Antigen retrieval effect is better in the solution of pH 7.0,8.0 than in that of pH 6.0.The best effect needs pH 8.0 sodium citrate solution .

本实验所用的4种组织，抗原修复液选择pH值7.0、8.0均优于pH值6.0，皮肤组织选择EDTA（pH值8.0），雌激素受体选择pH值8.0时染色效果最佳。

Antigen retrieval effect is better in the solution of pH 7.0,8.0 than in that of pH 6.0 . EDTA ( pH 8.0 ) appears better for skin . The best effect of Estrogen Receptor needs pH 8.0 sodium citrate solution .

为了提高ICA的阳性检出率，本研究应用了微波抗原修复术。

The microwave antigen repair technique was used to improve ICA positive detection rate .

作者应用c－fos、Rb蛋白抗体和微波炉抗原修复法对人慢性放射性皮肤溃疡进行免疫组织化学研究。

We performed an immunohistochemical study on human radiation skin ulcer by using antibodies against c-fos and Rb proteins and antigen-repairing method with a microwave oven .

结论：微波加热抗原修复可使甲醛固定石蜡包埋乳腺癌组织中的EABA重新暴露。

Conclusions Heat-induced antigen retrieval could unmask EABA in formalin-fixed and paraffin-embeded tissues of breast carcinoma .

对每一种抗原修复液，应用4个不同的抗原修复时间：12min、20min、25min和30min。

For each buffer , there were 4 durations for antigen retrieving , namely 12 , 20 , 25 and 30 min.

柠檬酸缓冲溶液（pH7.2）100℃抗原修复3h及一抗4℃孵育12h是空肠组胺免疫组化的良好条件；

It is the condition of immunohistochemistry that citric acid buffer fluid boiled for 3h to restore the antigen , and the antibody incubated with the section in 4 ℃ for 12 h.

结论用枸橼酸缓冲液（PH6.0）微波抗原修复组织样本及PV检测法是CD117免疫组化表达的较为理想的检测方法。

Conclusions it is the ideal method of detection of CD117 antigen that CD117 antigen was retrieved by citric acid buffer ( PH6.0 ) microwave antigen retrieval and detected by PV staining .

超声波对免疫组化抗原修复的作用

The effect of Supersonic wave on the repairing of histochemical antigen

各种癌基因蛋白微波抗原修复免疫组织化学检测的研究

Immunohistochemical studies of oncoproteins with antigen retrieval by microwave oven irradiation

结论：高压加热抗原修复法不失为一种简便有效的抗原修复方法，值得推广应用。

Conclusion : The method is useful rapid and simple .

3种抗原修复法对免疫组化结果的影响

The influence of three kinds of antigen retrieval methods for immunohistochemical results

不同抗原修复方法对肝癌组织中内源性生物素的影响及对策

Influence of different heat-induced epitope retrieval methods on endogenous biotin in hepatocellular carcinoma tissues

热抗原修复方法的比较

The Comparison of Heating Induced Epitope Retrieval Methods

目的探讨不同的抗原修复方法对免疫组化染色结果的影响。

Objective To explore different methods of antigen retrieval which affect the immunohistochemical staining results .

应用微波进辐射进行抗原修复和免疫荧光检测。

Applying the microwave performs the antigen recovery and immunofluorescent staining detection . Results : 1 .

定量比较不同抗原修复条件对免疫组化阳性表达的影响

Quantitative comparison of effects of antigen retrieval under different conditions on positive expression detected by immunohistochemistry

抗原修复脱钙组织石蜡切片抗原性后免疫组织化学染色效果的比较

Comparison Between Different Ways of Antigen Retrieval on Immunohistochemistry Staining of Antigenicity of Paraffin Section of Decalcifying Tissue

微波抗原修复，92-98℃×15分钟，室温冷却；

Put them into microwave oven to repair antigens for 15 min at 92-98 ℃, cooled at room temperature ;

加热抗原修复对内源性抗生物素蛋白结合物的影响及其对策

The influence of heat-induced epitope retrieval on endogenous avidin-binding activity ( EABA ) and blocking of EABA in immunohistochemistry

结论免疫组化的染色质量受不同抗原修复方法的影响。

Conclusion The quality of immunohistochemistry staining is affected by deferent antigen repairing method as well as the pH of repairing solutions .

采用高压锅抗原修复法，其阳性表达率为47.4%，脱片率为20.0%；

Using the pressure cooker antigen repairing method , the positive expressing rate is 47.4 % and then unfalling rate is 20.0 % ;

标本常规脱蜡水化后，进行抗原修复，继以免疫组化试剂盒进行检测。操作步骤按说明书进行。

After routinely dewaxing and hydration , antigen in specimens proceeded to be repaired according to statement by steps on the immunohistochemistry of kits for testing .

目的探讨不同抗原修复方法对肝癌组织中内源性生物素的影响以及消除办法。

Objective To investigate the influence of different heat-induced epitope retrieval methods on endogenous biotin , and to establish an effective way to block endogenous biotin in hepatocellular carcinoma .

结果加热抗原修复暴露肝细胞癌组织中内源性生物素的活性。定量比较不同抗原修复条件对免疫组化阳性表达的影响

Results The activity of endogenous biotin was revealed after the hepatocellular carcinoma tissues were treated with microwave heat-induced epitope retrieval . Quantitative comparison of effects of antigen retrieval under different conditions on positive expression detected by immunohistochemistry

bFGF/BMP/脱抗原骨复合物修复股骨头骨缺损的实验研究

Treatment of Femoral Head Defects by bFGF / BMP / Antigen-extracted Bone : An Experimental Study

结论：不同的抗原有其各自最适合的抗原修复液、pH值范围和最适修复时间。

Conclusion : Different antigens have their own optimal retrieval buffers and the optimal retrieval time .