抑制消减杂交

抑制消减杂交和基因芯片技术是筛选差异表达基因的有效方法。

SSH and cDNA array are efficient methods to discover the differential expressed genes .

结论抑制消减杂交技术是筛选、克隆大肠癌转移相关新基因的有效手段；

Conclusion SSH is a reliable strategy for screening novel genes differentially expressed in metastasis of colorectal carcinoma .

长角血蜱雄蜱抑制消减杂交cDNA文库的构建与分析

Construction and analysis of SSH cDNA library of male Haemaphysalis longicornis

抑制消减杂交法分离血管内皮细胞中表达的TNFα相关基因

Isolation of TNF α Related Genes Expressing in the Endothelial Cells by Suppression Subtractive Hybridization

方法抑制消减杂交（SSH），cDNA芯片。

Methods Suppression subtractive hybridization ( SSH ) and cDNA microarray were performed .

运用抑制消减杂交构建辐射诱导EST库

Suppression subtractive hybridization in construction of radiation - induced EST library

cDNA微阵列和抑制消减杂交技术筛选胃癌下调功能基因组的研究

Down-regulated genes screening and novel genes cloning by cDNA microarray and suppression subtraction library in gastric cancer

方法ELISA、冠状病毒全基因组芯片、抑制消减杂交（SSH）。

METHODS ELISA , whole genomic gene chip of coronavirus , and SSH were used .

抑制消减杂交（SSH）及其在鱼类基因克隆中的应用

Suppression subtractive hybridization and its application to fish gene cloning

利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST

Isolation of Fertility Restoration-related ESTs in Pepper Cytoplasmic Male Sterility Lines Using SSH

结果：通过反向mRNA斑点印迹技术可以从抑制消减杂交阳性克隆中把差异表达基因有效地筛选出来。

Results : The differential expression genes are efficaciously screened from suppression subtractive hybridization positive clones using reverse mRNA dot blot technique .

应用抑制消减杂交和cDNA表达谱芯片筛选肝星形细胞持续活化的相关基因

Combination of suppresion subtractive hybridization and cDNA microarray for identification of the hepatic stellate cell continual activation related genes

结论用抑制消减杂交法构建的传代培养前后主动脉内皮细胞差异表达基因消减cDNA文库富含动脉粥样硬化相关基因。

Conclusion The subtracted cDNA library constructed by SSH technique contains the atherosclerosis related genes when the aortic endothelium were cultured .

目的应用抑制消减杂交技术（SSH）构建胰腺癌和正常胰腺组织间差异表达的抑制消减cDNA文库。

Objective To construct a suppressive subtractive cDNA library of differentially expressed genes of human pancreatic cancer and normal pancreas tissues by suppressive subtractive hybridization ( SSH ) .

方法利用抑制消减杂交技术，构建大鼠轻度肝纤维化和重度肝纤维化2个差异cDNA文库，并挑选36条上调显著的基因进行测序鉴定。

Methods Two cDNA subtractive libraries of genes differentially expressed among normal hepatic tissues , gentle liver fibrosis tissues and serious liver fibrosis tissues were constructed by suppression subtractive hybridization technique .

抑制消减杂交（SSH）与基因芯片技术是筛选差异表达基因的新方法，因其快速、高效等特点而得以广泛应用。

Suppression subtractive hybridization ( SSH ) and gene chip are new technologies developed in recent years , which are used to screen differential expression genes .

建立鸽左侧单眼剥夺模型，采用抑制消减杂交和反Northern方法筛选右视盖差异表达的表达序列标签。

The left monocular deprivation model of pigeons was established . By using suppression subtractive hybridization technique and reverse Northern assay , the differentially expressed sequenced tags ( ESTs ) for right optic tecta of pigeons were screened .

采用抑制消减杂交方法，以褐飞虱取食32h的水稻幼苗及未受褐飞虱取食的水稻幼苗为作为对比材料构建了消减cDNA文库，以分离水稻幼苗中褐飞虱应答基因。

Suppression subtractive cDNA library was constructed by using rice seedlings exposed to BPH for 32 h and control plants as materials .

在这些技术当中，抑制消减杂交（SSH）具有快速、高效、假阳性率低等特点。

Among these techniques , the suppression subtractive hybridization ( SSH ) method has been shown to be quick and highly efficient , and it generates less false-positive clones .

本文综述了抑制消减杂交（suppressionsubtractivehybridization，SSH）的基本原理、技术路线、基本步骤、优缺点和在畜禽功能基因组研究中的应用情况。

The suppression subtractive hybridization ( SSH ) technique was discussed in this article , including its principle , procedure , advantage , disadvantage , and application on functional genome study in animal .

方法提取胰腺癌和癌旁正常胰腺组织中的RNA和mRNA，应用抑制消减杂交技术（SSH）构建消减cDNA文库。

Methods Total RNA and mRNA were isolated from human pancreatic cancer ( as tester ) and normal paracancerous pancreas tissues ( as driver ), respectively . The suppressive subtractive cDNA library was set up using suppressive subtractive hybridization ( SSH ) .

应用抑制消减杂交法（SSH）筛选出在正常心脏组织表达，但在异常心脏组织中表达减少或不表达的基因，构建减数杂交文库，用cDNA芯片技术验证，并进行测序。

Suppression subtractive hybridization ( SSH ) was used to discover messages decreased or not expressed in outflow tract tissues without cardiac neural crest cells . A subtractive library was constructed and cDNA chip was used to identify significant difference .

以同一水稻品种接种不同小种稻瘟菌，利用抑制消减杂交（SSH）技术构建水稻-稻瘟菌非亲和/亲和互作消减cDNA文库。

RNAs isolated from plants of a rice cultivar inoculated with two different races of the blast fungus were used to construct two cDNA libraries enriched for transcripts differentially expressed in incompatible / compatible interactions through suppression subtractive hybridization ( SSH ) .

抑制消减杂交技术（suppressionsubtractivehybridization，SSH）是在转录水平上研究基因差异表达的技术，具有高特异性、高灵敏性和高效率等优点，可作为筛选差异表达基因的技术。

Suppression subtractive hybridization ( SSH ) study differential gene expression at the transcriptional level , because of its high specificity , high sensitivity and high efficiency , therefore , it can be as a powerful means to screening differentially expressed genes .

结论：经抑制消减杂交建立的消减cDNA文库富集并均一化了内毒素刺激后不同丰度的差异表达基因，对深入理解内皮细胞活化机制及参与组织修复的过程具有重要意义。

CONCLUSION : The subducting cDNA library constructed by suppressive subducting hybridization enriched and homogenized the differentially expressed genes of different degrees of abundance stimulated by endotoxin . It is important for understanding the activation mechanism of endothelial cells and their role in the process of tissue repair .

目的：利用抑制消减杂交（suppressionsubtractivehybridization，SSH）和cDNA表达谱芯片筛选早期活化的肝星状细胞与大鼠正常肝脏组织、轻度肝纤维化组织、重度肝纤维化组织中差异表达的基因。

Objective To screen and identify the differentially expressed genes in the continually activated hepatic stellate cells , the normal hepatic tissues , the mild liver fibrosis tissues , as well as the serious liver fibrosis tissues using suppression subtractive hybridization ( SSH ) and cDNA microarray .

方法以甲基亚硝基胍（MNNG）为受试物诱导腭裂动物模型，采用抑制消减杂交技术从全基因组水平上对MNNG致腭裂相关差异表达基因进行筛选。

Method N-methyl-N ' - nitro-N-nitrosoguanidine ( MNNG ) was used as a known teratogen to induce cleft palate in ICR mice and a suppression subtractive hybridization ( SSH ) technique was applied to identify differentially expressed genes which related to cleft palate in ICR mice .

应用抑制消减杂交技术克隆鼻咽癌转移调控基因

Cloning of metastasis-associated genes in nasopharyngeal carcinoma using suppression subtractive hybridization

抑制消减杂交法筛选无症状乙型肝炎病毒携带者差异表达基因

Screening differentially expressed genes of asymptomatic HBV carriers by suppression subtracted hybridization

抑制消减杂交技术研究进展及其在环境医学上的应用

Improvement of Suppression Subtractive Hybridization and Applications on Environmental Medicine