慢病毒感染

然后通过原核显微注射和慢病毒感染等不同方式来制备转基因小鼠。

Transgenic mice were generated by either microinjection or lentivirus infection . The expression level of human transferrin was analyzed and the efficiencies of two different preparation methods were studied .

结论1.系统建立了完整的人脐带血CD34+细胞分离、无血清无基质培养和慢病毒感染的实验方法。

Establish a set of methods for separation , serum-free and stroma-free culture , and infection of human CB CD34 + cells . 2 .

由于慢病毒感染宿主细胞后，其基因可以高效的整合到宿主染色体中，因此可以在宿主细胞内持续稳定的表达外源基因。

After infecting host cells , its genome can be integrated into the host genome , resulting in the stable expression of exogenous genes .

为了研究受精过程对慢病毒感染胚胎的影响，本试验采用卵周隙显微注射的方法，用低滴度的病毒（106TU/ml）分别感染小鼠受精卵和卵母细胞。

In order to study the influence of fertilization on the infection of embryos by lentivirus , lentivirus of low titer ( 106 TU / ml ) were microinjected into perivitelline spaces of mouse fertilized eggs and oocytes .

绵羊慢病毒自然感染绵羊的硬化性淋巴细胞性乳腺炎

Indurative Lymphocytic Mastitis in Sheep Infected Naturally with Ovine Lentivirus

慢病毒载体感染人皮肤成纤维细胞的研究

Infection of human foreskin fibroblasts by lentivirus vector

慢病毒能够高效感染处于静止期和分裂期的细胞，这使得它成为实现长期基因表达的理想工具。

Lentivirus can efficiently infect quiescent and division of cells , which makes it an ideal tool for long-term gene expression .