定点整合

新型CHO/dhfr~-细胞定点整合和表达系统的建立

Construction of targeted integration and high expression system in CHO / dhfr - cell line

小麦叶绿体基因组定点整合表达载体的构建

The Construction of Site-specific Integration and Expression Vector for Chloroplast Genome of Wheat

酿酒酵母位点特异性DNA切离及定点整合的研究

Studies on Site specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae

人TPO基因牛体细胞定点整合载体构建与细胞转染研究

A Preliminary Study on the Human TPO Gene Targeting Vector Construction and Bovine Somatic Cell Transfection

绵羊体细胞myostatin基因敲除及定点整合mAAT基因的初步研究

A Preliminary Study on the Knockout Myostatin and Knockin mAAT in Sheep Somatic Cells

感染后1个月及4个月检测，实验大鼠肝脏Southernblot杂交结果均为阳性，HCVCORE区基因非定点整合；肝细胞mRNA的dotblot杂交结果均为阳性，提示正确表达HCVCoremRNA。

One and 4 months after infection , all the results of Southern blot assay and Dot blot assay were positive , indicating that the integrated HCV core genes and HCV core mRNA existed in the liver of bandicoots .

目的：利用体细胞基因打靶技术来获得定点整合有重组人瑞替普酶（rPA）的山羊胎儿成纤维细胞株。

AIM : To obtain a goat fetal fibroblast cell line with site-directed recombination of reteplase ( rPA ) minigene by somatic cell gene targeting technique .

并将一个含有FRT重组位点的环状质粒整合到酿酒酵母染色体上预先设置的一个FRT重组位点上，实现了染色体定点整合。

Moreover , a circular plasmid containing a FRT site was site specifically integrated into chromosome via site specific recombination with a preset FRT site on the chromosome .

本实验成功应用原代成纤维细胞基因打靶技术，首次获得了在β-酪蛋白基因座定点整合IL-11的GEF细胞株，为进一步核移植做好了准备。

The gene-targeting technique in primary fibroblast was successfully used in GEFs in this experiment , and the cell lines were finally obtained that the human IL-11 gene was integrated in 3 - casein gene locus of goat .

目的：构建含有FRT序列的CHO/dhfr-工程细胞株，并在此细胞株中用定点整合系统表达抗基孔肯亚病毒人鼠嵌合抗体。

AIM : To obtain CHO / dhfr ~ - cells line with integrated FRT sequence in the chromosome transcription active site and to express human-mouse chimeric antibody directed against Chikungunya Virus by using the cell line .

结论：构建了含有FRT序列的CHO/dhfr-工程细胞株，并在此细胞株中用定点整合系统表达抗基孔肯亚病毒人鼠嵌合抗体，为用此系统表达抗体分子和其他蛋白分子奠定了基础？

CONCLUSION : A cell line with integrated FRT sequence in the chromosome transcription active site was obtained and with it human-mouse chimeric antibody directed against Chikungunya virus was expressed , it lays a solid foundation which can be used for expressing antibodies and other proteins .

斑马鱼胚胎细胞中Cre/lox介导的定点整合

Cre / lox - mediated Site - specific Recombination in Zebrafish Embryos

番茄质体多顺反子定点整合表达载体的构建及其转烟草的研究

Construction of Tomato Chloroplast Multicistron Site Integration Expression Vector and Its Transgenic Tobacco

用定点整合系统表达抗基孔肯亚病毒人-鼠嵌合抗体的研究

Expression of human-mouse chimeric antibody directed against Chikungunya virus with site-specific intergration system

运动发酵单胞菌定点整合质粒的构建

Construction of a Specific Integrating Plasmid for Zymomonas mobilis

安全环保的烟草质体多顺反子定点整合表达载体的构建

Construction of The Safely and Friendly Environmental Tobacco Chloroplast Multicistron Site Integration Expression Vector

定点整合抗虫基因到油菜叶绿体基因组并获得转基因植株

Site-specific Integration of Insect-resistant Gene into Chloroplast Genome of Oilseed Rape and Acquisition of Transgenic Plants

菊苣质体同源片段的克隆及非抗生素标记的质体定点整合表达载体构建

Cloning of homologous targeting sequences and construction of antibiotic-free plastid site-specific integration expression vector of chicory

重组人瑞替普酶基因在山羊胎儿成纤维细胞β-酪蛋白基因座的定点整合

Site-directed Recombination of Human Reteplase at the β - casein Loci of Goat Primary Fetal Fibroblasts

构建了山羊基因组文库，为筛选山羊乳蛋白基因，构建山羊乳蛋白基因定点整合的转基因敲入载体奠定基础。

Goat genomic DNA library was constructed for screening milk-protein-encoding gene of goat and construction of knock-in vector .

定点整合型基因治疗腺病毒载体的构建

Construction of adenovirus vector for gene therapy which can integrate foreign gene into host cell genome at specific site

转基因鱼不育系的培育和外源基因的定点整合是解决这两个问题的主要方法，同时也是转基因鱼食用安全性评价和检测的基础。

Getting sterile triploid transgenic fish and fixed point integration are efficient to solve the two problems above respectively .

目的尝试水稻质体多顺反子定点整合表达载体转化到烟草质体中表达。

【 Objective 】 The rice ( Oryza sativa ) chloroplast multicistron site integration expression vector will be transformed for expression into the tobacco chloroplast .

摘要：叶绿体遗传转化相较于核遗传转化具有诸多优点，包括高拷贝、定点整合、环境安全性好等。

Abstract : Compared with nuclear transformation , chloroplast transformation has a number of unique advantages , such as fixed-point integration , high copy and environmental security .

针对转基因鱼技术中关于启动子和结构基因的选配、外源基因的定点整合、外源基因的可控表达、转基因鱼鉴定、建立转基因鱼纯系个体以及转基因鱼应用安全问题几个方面进行了论述。

The article discuss some question in study of transgenic fish , including choosing of promoter and structure gene , controling expression of outer gene , identify of transgenic fish and the safety of transgenic fish .

结果：获得了一株定点整合的细胞克隆，绝对基因打靶效率为2×107。结论：本实验结果为后续利用核移植技术制备乳腺生物反应器提供了坚实的实验基础。

The absolute frequency of homologous recombinant event is 2 × 10 - 7 . CONCLUSION : This preliminary investigation provides a basis for future studies to generate mammary gland bioreactors through the subsequent nuclear transfer ( NT ) technique .

为了方便转基因小鼠的构建和转基因功能的分析，我们设想发展一种高效、定点、单拷贝整合的转基因系统。

To facilitate the construction of transgenic mice , we planed to develop an efficient , she-specific transgenic mice producing system .