微流控技术

微流控技术（microfluidics）由于可望实现大大降低试剂消耗，缩短反应时间，减少费用，众多的集成潜力，正在成为新一代生物分析技术的重要推动力。

Microfluidics is expected to have many advantages such as greatly reducing reagent consumption , shortening reaction time , reducing costs , and have high potential integration , therefore it is becoming one of important driving forces in the development of a new generation of bio-analytical techniques .

微流控技术是在几十到几百微米尺度的通道中，进行处理和操纵纳升甚至更小体积液体的一种新兴技术。

Microfluidics is an emerging technology featured by processing and manipulation of nano-volume liquid , or even smaller , in tens to hundreds of micro-scale channels .

在微流控技术日益朝生物领域发展的过程中，PCR微流控技术始终是研究的热点之一。

The microfluidics based PCR technology is always one of the hotspots in the increasing development of the microfluidics technology for the biological fields .

基于微流控技术蛋白质结晶芯片制作工艺的研究

Study on Fabrication Process of Protein Crystallization Microfluidic Chip

基于微流控技术新型光开关器件设计

Design of New Optical Switches Based on Microfluidic Technology

微流控技术应用于蛋白质结晶的研究

Research and Application of Microfluidics in Protein Crystallization

近年来，以液体作为主要工作介质的液体光学系统成为微流控技术一个重要发展方向。

The liquid optical system using liquid as working medium becomes a promising topic in the Microfluidic Technology .

微流控技术在过去的二十年开始兴起，并且已经实现了对微米通道中流体的控制。

The microfluidic technology which developed in the past two decades has already fulfilled the fluid control in the microfluidic channels .

结合微流控技术，在同一基底上也可以达到多种细胞的共培养。

In combination with the microfluidic system , adhesion of multiple types of cells on a same substrate can be carried out .

本工作的主要内容是基于微流控技术的化学发光生物传感器芯片以及新型化学发光体系的研究，全文由二部分组成。

In the present work , the investigation on the biosensor chip with Chemiluminescence detection and novel Chemiluminescence systems is reported in part I and II , respectively .

全文共四章：第一章简述了微流控技术，包括芯片的制作以及芯片在电泳分析中的应用。

There are four chapters in this thesis : Chapter ⅰ, Microfluidics , including the production of chips and and the application of chip electrophoresis has been reviewed .

微流控技术与光学器件的融合，为传统光学器件的微型化、阵列化、低成本化以及高精度控制提供了可能。

It is possible that the miniaturization , arraying , low-cost and precise control of the optical devices are realized by the cooperation between microfluidic technology and optical devices .

基于毛细管电泳的微流控芯片技术可以更快地完成PCR产物分离。

Capillary electrophoresis by microfluidics is an alternative method for more rapidly separating such as PCR products .

对微流控电泳技术在DNA样品中的应用进行了阐述，总结了用于DNA分离的微流控设备、筛分介质种类、筛分介质性质以及常见染料对分离的影响。

Microfluidic chip electrophoretic separation of DNAs was introduced and the affects of microfluidic devices , type and property of sieving medium as well as common dyes for DNA analysis were summarized .

随着MEMS和微流控芯片技术的快速发展，光敏玻璃凭借其特有的优点，在微器件领域开始得到广泛应用。

With the rapid development of MEMS ( Micro-Electro-Mechanical Systems ) and microfluidic chip technologies , photosensitive glass has been widely used in micro machining field for its own advantages .

将HT显微镜与微流控分析技术联用，发展了一种单细胞进样与在线成像分析新技术，并将其应用于花粉单细胞成像分析方面。

By combining the HT microscopy with the microfluidic analysis technique , a novel method for single-cell sampling and on-line imaging is developed . This method is applied to single-cell imaging analysis of pollen cells .

一种基于微流控芯片技术的流式细胞计数系统

Cell counting system with flow cytometry based on microfluidic chip

微流控芯片技术及其在检验医学中的应用

Microfluidic chip technique and its application on laboratory medicine

本文依托微流控芯片技术，选取悬浮细胞为研究对象，开发一系列的微流控芯片。

On the basis of microfluidics , a serial of microfluidic chips were developed forthe research of suspension cells .

微流控芯片技术的微米级尺寸以及不同单元操作、灵活组成和自由集成的特点为细胞生物学提供了一个理想的研究平台。

The technology of microfluidic chip provides a suitable research platform for cell biology due to its micro scale , flexible composition and easy integration .

与传统的硅材质芯片相比，高聚物材料芯片具有易于加工，性质稳定以及成本低廉等众多优点，所以近年来高聚物芯片的制作和应用成为了微流控芯片技术的发展方向。

Compared with the traditional silicon chips , polymer ones have the advantages of easily fabrication , stable properties and low cost , and so on .

微流控芯片技术具有制备简单、试剂用量少、操作方便等优点，因此在生化分析中的应用越来越受到重视。

Microfluidic chip technology has the preparation of simple , low reagent use , easy to operate , etc. , so in the biochemical analysis of the growing attention .

微流控芯片技术是一个跨学科的新领域，它是新世纪分析科学、微机电加工、生命科学、化学合成、分析仪器及环境科学等许多领域的重要发展前沿。

Microfluidic chip technology is a new territory in the new century , it is the frontier of analytical sciences , micro electromechanical process , life sciences , chemosynthesis , analytical instrument and environmental sciences etc.

为了能连续、高效地提取抗新型突发性病毒和增强人体免疫力的生化制品，需要开发一种结合凝胶色谱分离特点和微流控分离技术相结合的新型微分离器件。

A new micro-separation device combined characteristics of gel chromatogram method and technologies of microfluidic chips is developed , in order to produce biochemical drug for resisting new virus and enhancing human immunity consecutively and effectively .

微流控芯片技术是分析化学领域的一种新兴研究技术，现已经扩展到生命科学、医药诊断、环境科学、军事刑事科学等领域，并得到广泛的研究和应用。

Microfluidic chip technology is a new research tool emerging from the field of analytical chemistry , which has been extended to the fields of life sciences , medical diagnostics , environmental science , military and criminal science and so on , and has been widely studied and applied .

PDMS微流控芯片加工技术及微萃取系统的实验研究

Experimental Study on Fabrication of PDMS and Microextraction System

近年来微流控芯片检测技术得到了长足的发展。

Recently , the microfluidic chip detection technology has been developed rapidly .

微流控芯片检测技术进展

Developments in Detection Techniques for Microfluidic Devices

微流控液滴技术以其样品消耗少、反应条件稳定、混合快速等特点，为蛋白质结晶条件筛选提供了强有力的技术平台。

With the characteristics of low sample consumption , stable reaction conditions , rapid mixing , microfluidic droplets provide a strong technical platform for screening of protein crystallization conditions .

随着微流控芯片分析技术的发展，微流控芯片中的关键技术之一流体的驱动和控制技术逐步成为人们关注和研究的热点。

With the development of the microfluidic analytical system , microfluidic driving and controlling technique , as one of its key techniques , is concerned and researched by relative experts .