尿酸酶

聚-2-氨基吡啶修饰电极比聚苯胺尿酸酶电极更稳定，因为聚苯胺尿酸酶电极受尿酸酶活性的影响。聚-2-氨基吡啶对尿酸的催化反应活化能Ea为23kJ。

Poly-2-aminopyridine electrode is more stable than polyaniline-uricase electrode , because the latter is affected by uricase activity .

结果：经SDS-PAGE检测重组尿酸酶亚基的表观相对分子质量为32.4k。

Molecular weight of the recombinant uricase subunit was 32.4 ku in SDS-PAGE .

UA含量测定采用尿酸酶-过氧化物酶偶联法。

The uric acid concentration was measured by uricase peroxidase method .

方法：用尿酸酶POD终点法，在全自动生化分析仪上测定血尿酸浓度。

Methods : The level of uric acids was tested by biochemical auto analyzer with uric acid enzyme POD terminal method .

目的：以Candidaspecies尿酸酶为模型，考察用积分法筛选抑制剂的条件。

AIM : To investigate the prerequisite of the integrated method for characterizing inhibitors with the inhibition of xanthine on Candida species uricase as the model .

在一定的条件下，尿酸酶的最适pH和酸碱稳定pH范围分别为8.5和5.0~12.最适温度为37℃；

On the stated condition , the optimum pH and range of pH stability were 8.5 and 5.0 ~ 12.0.The enzyme showed good thermal stability with 37 ℃ as its optimum temperature .

人尿酸酶基因是由本实验室从人肝脏组织中提取的，保存在大肠杆菌BL21（DE3）菌株中。

The human uricase gene is extracted by this laboratory from human liver organization , and preserved in BL21 ( DE3 ) expression strain .

结果采用灌胃给予次黄嘌呤，皮下注射以羊毛脂石蜡油为溶剂配制的尿酸酶抑制剂，可使受试动物血尿酸值明显高于空白对照组（P<0.01），且可维持12h。

Results Hypoxanthine was administered via stomach , while uricase inhibitor was injected subcutaneously by lanolin mixed with olefin , which made the uric acid in serum higher than control group about 12 hours .

文章简要介绍了尿酸酶生物传感器研究的必要性及国内外研究现状；

The necessity and present conditions of uricase biosensor are introduced .

重组尿酸酶部分理化性质及其初步药效学研究

Study on Physicochemical Properties and Pharmacodynamic Action of Recombinant Uricase

尿酸酶产生菌的筛选、基因克隆及表达

Screening of Uricase Producing Strains , Cloning and Expression of Uricase Gene

掺杂-涂膜聚苯胺尿酸酶电极的生物电化学特性

Bioelectrochemical Characters of Doping - coating Polyaniline - uricase Electrode

苛求芽孢杆菌尿酸酶的表征、应用与其基因克隆

Characterizing , Application and Cloning of Uricases from Bacillus Fastidious

用线性动力学方法考察黄嘌呤对尿酸酶的抑制作用

Inhibitory effects of xanthine on uricase analyzed by a linear kinetic method

茶碱对聚苯胺尿酸酶电极的生物电化学活性的抑制

Inhibition of Bioelectrochemical Activities of Polyaniline Uricase Electrode by Theophylline

产朊圆酵母尿酸酶的分离纯化及其部分性质研究

Study on Purification and Characterization of Uricase from Candida utilis

聚苯胺尿酸酶电极性能的研究

The study of properties for the polyaniline uricase electrode

硫酸铵沉淀尿酸酶的最佳浓度为75%。

75 % ammonium sulfate fractionation can get the most uricase activity field .

方法：用293nm吸收记录尿酸酶反应。

METHODS : Uricase reaction curve was monitored by absorbance at 293 nm .

人类尿酸酶基因因突变而成为假基因，如果能使之复活，必然能够在很大程度上降低该酶的免疫原性。

Uricase gene mutations of human may result in the formation of pseudogenes .

尿酸酶抑制剂在大鼠急性高尿酸血症模型复制中的应用

Exploratory Development of Uricase Inhibitor for Replicating Acute Animal Model of Hyperuricemia on Rat

乳糖诱导重组尿酸酶基因在大肠杆菌中的表达

Expression of Recombinant Uricase in E. coli JM109 ( DE3 ) Induced by Lactose

聚吡咯尿酸酶电极的生物电化学活性

Bioelectrochemical Activities of the Polypyrrole Uricase Electrode

纳米颗粒增强尿酸酶生物传感器的研究设想

Study On The Nanoparticles Strengthen Uricase Biosensor

测定尿酸酶电极的研制

Study of Enzyme Electrode for Uric Acid

最终筛选到的人犬嵌合尿酸酶与初始犬源尿酸酶相比，活性和稳定性均有显著提高。

Both the activity and stability of the finalized uricases were improved compared to the original uricase .

苛求芽孢杆菌胞外尿酸酶的表征及其在血清尿酸测定中的应用

Characterization of an Extracellular Uricase from Bacillus fastidious and Its Application in Direct Kinetic Assay of Serum Uric Acid

本部分研究利用尿酸酶抑制剂氧嗪酸和抑制肾脏排泄尿酸的药物吡嗪酰胺，建立一种与人类高尿酸血症天津医科大学博士研究生学位论文更为接近的高尿酸血症动物模型。

In part 2 , a new hyperuricemia model that is more similar to patient is established by oxonic acid and pyrazinamide .

大多数哺乳动物都有一个叫做尿酸酶的基因来分解尿酸，但人类缺乏这种能力。

Most mammals have a gene that makes an enzyme called uricase that breaks down uric acid , but humans lack the ability .

实验结果发现：①该方可显著降低高尿酸血症模型小鼠（尿酸酶抑制剂造模法和次黄嘌呤造模法）的血尿酸水平。

The results showed : 1 . It could reduce the uric acid of hyperuricaemia animal model and inhibit xanthenes oxidation enzyme ( XOD ) .