以可溶性抗NP单链抗体包被酶标板，BSA封闭酶标板。
ELISA plate was coated with soluble scFv and was blocked with BSA .
5%H2O2甲醇振洗25分钟，以封闭内源性过氧化物酶活性；
Placed them in methanol containing 5 % H2O2 for 25 min to block endogenous peroxidase activity ;
封闭酶