封片

固定剂、封片剂、温度及除菌方式对量子点标记细胞的影响

Effect of fixative , mounting medium , temperature and sterilized method on cells by quantum dots

4HE染色石蜡切片经过二甲苯脱蜡，梯度酒精水化，苏木素-伊红染色，再梯度酒精及二甲苯脱水，封片。

HE staining paraffin sections xylene dewaxing , gradient ethanol hydration , hematoxylin-eosin stain , then gradient alcohol and xylene dehydration , mount .

目的研究国产依普拉封片（ipriflavone）防治绝经后骨质疏松症疗效的生化学监测及疗效机制。

OBJECTIVE To monitor the change of biochemistry in patients with postmenopausal osteoporosis after intaking ipriflavone and discuss its action mechanism .

方法自雌蚊腹部剪下腹节最后两节，经KOH浸泡过夜后，酒精脱水、二甲苯透明，加拿大树胶封片后显微镜下观察。

Methods The last two abdomen segments of mosquitoes were cut down and treated with KOH , alcohol and xylene . Then the samples were observed under Nikon light microscope after sealed with neutral balsam on slides .

将K562细胞培养于载玻片上，48h后用冷丙酮固定，然后PES洗3次，用混合荧光染料染色，荧光染料荧光染色、PBS水洗封片，镜检。

Culture K562 cell in slide , fix it with the cold acetone after 48h , then wash 3 times with PES , dye with the mixture fluorescence dye for 5 min , wash with PES , seal slice , examine with micros - cope .

国产依普拉封片防治绝经后骨质疏松症生化监测

Biochemistry monitoring in patients with postmenopausal osteoporosis after ipriflavone intake

苏木素浸泡，分化液分化，返蓝。中性树胶封片。

Haematoxylin immersion , differentiation liquid differentiation , Neutral gum sealing piece .

变速箱离合器片冲齿模具的设计毛细管铝封片冲挤工艺及模具

Stamping-Extrusion Technology And Die Design of Aluminum Seal Sheet

金属弹性封片将卸压阀卡压在卸压阀孔内。

The metal elastic sealing sheet presses the pressure relief valve inside the pressure relief valve hole .

对培养在塑料皿中的单层细胞直接染色时，最后的二甲苯透明和树胶封片两步要删除，以免影响最终的染色效果。

When dyed the cells on the plastic utensil directly , the last two step must omitted for influence the effect of dyeing .

经家兔角膜刮片用10%氢氧化钾封片镜检、角膜组织原虫培养和病理切片染色检查证实，成功地建立了家兔棘阿米巴角膜炎动物模型。

Acanthamoeba protozoa were identified by the methods of corneal scraping with 10 % potassium hydroxide wet mount examined under microscope , corneal protozoa culture and pathological section examination .

一部分标本固定后，采用抗γ和抗λ免疫球蛋白轻链单克隆抗体荧光免疫组化染色，封片镜检；

After fixation of part muscle sample , used monoclonal antibody fluorescent immunohistochemistry staining of anti - γ and anti - λ immune globulin light chain to research it .