天牛

天牛基因组DNA的提取方法

Extraction Method for Genome DNA from Longicorn Beetles

天牛干标本DNA的提取及RAPD-PCR扩增反应

DNA extraction of museum specimens and RAPD-PCR reaction of longicorn beetles

桑天牛成虫肠道细菌基因组DNA的提取及PCR反应体系优化

Genomic DNA Extraction and Optimization of PCR Reaction System of Apriona germari Intestinal Bacteria

天牛能否自身消化植物的纤维素，是长期来争论未决的问题，本课题用聚丙烯酰胺凝胶等电聚焦电泳方法着重研究了纤维素酶复合酶中CX酶在虫体中的来源。

The origin of the chief cellulose digesting enzyme , C_X-Cellulase , in longhorn beetles was investigated .

抗天牛基因cryⅢA的改造及人工合成

Modification and Artificial Synthesis of cry ⅲ A Gene Toxic to Longhorn beetles

为使天敌能大量人工繁殖，从分子生物学角度对单头桑天牛卵啮小蜂基因组DNA的提取方法及RAPD反应条件进行了探索。

In order to propagate more Aprostocetus prolixus , we studied the method of genomic DNA extraction and optimization of RAPD ana-lytic conditions .

通过试验探索出3种可以快速、简便地从天牛干标本、液浸标本和新鲜标本中提取DNA模板的方法。

An experiment was conducted to explore extraction methods for DNA from dry , dipped and fresh samples of longicorn beetles . Three methods to extract quickly and simply were obtained .

结果表明，喷雾使用CSⅡ不育剂20倍液对光肩星天牛的不育效果最好。

The results showed that the treatment spraying with CS ⅱ after diluting it 20 times gained best sterile effects on the pest .

以蚂蚁、天牛、蝗虫等昆虫为实验材料，建立了75%酒精常温浸泡密封保存和福尔马林常温浸泡密封保存昆虫样品2~4周后进行全基因组DNA提取的方法。

The method for extraction genomic DNA from insect sample , such as ants , longicorn beetles and grasshoppers , which stored in formalin or 75 % ethanol for 2 ~ 4 weeks , is described .

用分光光度法对毛白杨叶片、韧皮部内POD、PPO酶活性进行了测定，结果表明POD酶无论是在叶部还是韧皮部都与桑天牛危害程度呈正相关。

The result showed that : 1 . The activities of POD enzyme and PPO enzyme in the leaves and phloem of P. tomentosa was tested Using ultraviolet spectrophotometry .

利用RAPD技术和ITS-2序列研究11种天牛的亲缘关系

The Study of Determining the Relationship in Eleven Kinds of the Longicorn Beetles by Rapd Molecular Mark and Its-2 Sequence

分别采用称重法和光合作用分析仪测定了白僵菌Beauveriabassiana侵染对桑天牛Aprionagermari幼虫营养生理及呼吸速率的影响。

The effect of Beauveria bassiana on the nutritional physiology and respiration of Apriona germari larvae was studied .

A-3型松褐天牛引诱剂诱虫谱研究

Study on the species of insects trapped by A-3 sawyer beetle attractant

为更好地进行区分，同时对天牛科2亚科7属11种昆虫进行了RAPD研究。

In order to distinguish them better , the method of RAPD was used at the same time to study 11 species of Cerambycidae , which belong to 7 genera , 2 subfamilies .

A-3型松褐天牛引诱剂监测松树主要蛀干害虫林间种群动态研究

Population Dynamic Monitoring of Principal Pine Stem-borers in Forest by A-3 Sawyer Beetle Attractant

天牛触角嗅觉感受的神经细胞（RNs）有3类，气味信息经神经细胞群传输至中枢神经系统，神经信号按标记路线或交叉纤维样式输导。

RNs of cerambycid olfactory sensilla located on the antennae can be classified as 3 types . The odour information is passed to the CNS by neurone populations .

该引诱剂引诱松褐天牛成虫的引诱活性与原型引诱剂基本一致，其林间有效作用半径和诱获率分别为70m和43.8%。

The attractive activity of this attractant to M.alternates was similar with prototype attractant and the effective attractive radius and trap ratio was70 m and43.8 % in forest , respectively .

用1：10氯仿、酒精混合液处理桑虎天牛幼虫2h对寄生率、单雌产卵量有明显的促进作用，显著优于未经处理的寄主；

On hosts treated with mixed solution of chloroform and alcohol ( 1:10 ) for 2 hours , parasitism rate and fecundity per female were higher than those on control hosts , but period of pre-oviposition prolongs and sex ratio decreases .

因此利用450W高压汞灯监测松墨天牛及松材线虫种群有应用价值，有待进一步试验完善。

It is suggested that 450W ( GGC ) light be applied in monitoring population dynamics of JPS and PWN after further test .

家茸天牛〔Trichoferuscampestris（Faldermann）〕近年来己成为一种新的、主要的仓储害虫在集安市部分乡镇粮库直接危害玉米，被害率达15%左右。

The cerambycids , ( Trichoferus campestris ) have been a new main stored-grain pest in recent years . The damage rate of stored corn was about 15 percent .

在室温下，将成对的天牛放在盖有铁纱网的直径25cm高25cm的塑料桶中进行试验。

The testing paired beetles were put into the plastic containers with diameter of 25 ? cm and 25 ? cm in height and covered with iron screen .

首次提出反映不同树种受天牛危害状况的综合危害指标，并以综合危害指标大小评价多树种配置模式的优劣，IDI越小该模式越好。

The IDI reflecting the general damage by ALB was put forward for the first time , and the disposal model was evaluated by the IDI . The less the IDI was , the better the disposal model was .

常见杨树天牛分类管理系统（CPLTMS）的研建

The establishment of the management system of taxonomic of poplar longicorn

室内双条杉天牛幼虫体上的每一个蒲螨膨腹体，平均可以产生125个后代。

Every female Pyemotes sp . can procreate 125 offspring indoors .

光肩星天牛对不同柳树选择性的研究

Research on the Selectivity of the Beetle for the Different Willows

桑天牛成虫平均致死取食面积369mm2。

The average adult death Apriona germari feeding area 369 mm2.3 .

提出天牛防治方法。

The method for control of long-horn beetle is put forward .

印楝提取物防治桑天牛的林间试验

Field experiment on the control of Apriona germari with neem extract

描述了该天牛各个虫态在发育过程中的一系列行为特征。

Behavior characteristic of each stage of this specie was described .

应用马尔科夫链预测杨树天牛发生量

Forecasting the damage area of Poplar Longicorn Beetles with Markov chain