多拷贝基因

多拷贝基因的存在被认为是粘细菌适应其复杂社会学行为的结果。

Duplicated genes were reported that could be helpful in adjusting myxobacteria to its complicated social behavior .

结论：该方法能方便高效地获得所需的多拷贝基因，为进一步克隆到表达载体并进行高效表达打下基础。

CONCLUSION : With this method we can conveniently and efficiently obtain the desired multi-copy gene , which lays the basis for the cost-effective expression .

人α心钠素多拷贝基因在大肠杆菌中的高效表达

CLONING MULTIPLE COPIES OF A GENE FOR HUMAN ATRIAL NATRIURETIC FACTOR Expression of α human atrial natriuretic peptide from multi jointed genes in Escherichia coli

我们发现多拷贝基因比单拷贝基因具有较少的AS形式，而且基因AS形式的数量与基因家族的大小存在负相关性。

We observed that duplicate genes have fewer AS forms than that of single-copy genes , and that a negative correlation exists between the mean number of AS forms and the gene family size .

利用两种基因的特定引物及总RNA进行的引物延伸反应产物在电泳的凝胶上均呈现一条十分清楚的带，表明这两种多拷贝小麦基因的转录分别起始于一个核苷酸。

One distinct band was detected in each primer extension reaction with total RNA extracted from wheat leaves and the primers specific for the wheat genes .

杂交结果为蝎毒素基因可能通过DNA重排、多拷贝或多基因家族来调控基因表达提供了证据。

Southern hybridization analysis showed that there were two specific hybridization signals , which indicated that scorpion regulated the expression of toxin genes using the mechanism of DNA rearrangement , multicopy or multiple gene family .

同时，从文库中获得任一单拷贝或多拷贝大熊猫基因的概率为99.93%。

The probability of any giant panda gene being found in this library should be about 99.93 % . 2 .

目的：构建蛇毒锯鳞蝰素（Echistatin，Ecs）多顺反子串联多拷贝基因。

Objective : To construct a polycistron tandem repeated Echistatin ( Ecs ) gene .

经RT-PCR分析，低拷贝数的转基因后代显阳性反应，多拷贝数的显阴性反应，说明T-DNA多拷贝数影响外源基因的表达。

Through RT-PCR analysis , low copy number transgenic plants showed positive reaction and multi-copy number showed negative reaction . It was suggested that the expression of foreign gene was influenced by the T-DNA copy number .